LOCUS       sequence-457335-        7615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full length mouse FoxP3 (R337Q).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7615)
  TITLE     Hur lab plasmids 2024
REFERENCE   2  (bases 1 to 7615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV-FoxP3-R337Q-IRES-Thy1.1
FEATURES             Location/Qualifiers
     source          1..7615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(359..378)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(506..528)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(722..741)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     841..863
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(901..919)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        987..1091
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1092..1160,1161..1952)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1310..1329)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2123..2711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2612..2631
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2865..2882
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2999..3020
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3035..3040,3041..3058,3059..3065)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3073..3089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3251..3765
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(3821..4309)
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     misc_feature    4357..4909
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4359..4925
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4519..4538)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4748..4765
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    4959..4983
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(6325..6351)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6370..6786)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(6386..6402)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6418..6440)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6853..7194
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(7258..7615,1..159)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 aactgcttac cacagatatc ctgtttggcc catattctgc tgtctctctg ttcctaacct
       61 tgatctgaac ttctctattc tcagttatgt attttccatg ccttgcaaaa tggcgttact
      121 taagctagct tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta
      181 aataaaatct tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt
      241 tgagtcaaaa ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatcgc
      301 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
      361 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
      421 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
      481 ttgcatgcac cattccttgc gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
      541 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
      601 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
      661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
      721 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
      781 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      841 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
      901 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      961 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1021 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1081 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1141 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1201 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     1261 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     1321 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     1381 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     1441 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     1501 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     1561 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     1621 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1681 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1741 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1801 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1861 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1921 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1981 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2041 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2101 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2161 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2221 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2281 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2341 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2401 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2461 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2521 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2581 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2641 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2701 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2761 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2821 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2881 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     2941 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3001 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     3061 tgttgtgtgg aattgtgagc ggataacaat ttcacaattc tccccagctc tttctgcgct
     3121 gtccataatg aactatttca ggagtattgg agtatgtgga ttcaaaacag tggctaattg
     3181 agtcaggatt acttaatatt cgactcctac cctcagaatg ttgttctcca acttcaagaa
     3241 actgttaccc atgaaagacc cccgctgacg ggtagtcaat cactcagagg agaccctccc
     3301 aaggaacagc gagaccacaa gtcggatgca actgcaagag ggtttattgg atacacgggt
     3361 acccgggcga cgcagtctat cggaggactg gcgcgccgag tgaggggttg tgggctcttt
     3421 tattgagctc ggggagcaga agcgcgcgaa cagaagcgag aagcgaactg attggttagt
     3481 tcaaataagg cacagggtca tttcaggtcc ttggggcacc ctggaaacat ctgatggttc
     3541 tctagaaact gctgagggcg ggaccgcatc tggggaccat ctgttcttgg ccctgagccg
     3601 gggcaggaac tgcttaccac agatatcctg tttggcccat attctgctgt ctctctgttc
     3661 ctaaccttga tctgaacttc tctattctca gttatgtatt ttccatgcct tgcaaaatgg
     3721 cgttacttaa gctagcttgc caaacctaca ggtggggtct ttcattcccc cctttttctg
     3781 gagactaaat aaaatctttt attttatcga attctacgcg tcacagagaa atgaagtcca
     3841 gggcttggag gagggagagg gaaagcagca gcagcagcat ccaggatgtg ttctgaacca
     3901 gcaggcttat gccgccacac ttgaccagct tgtctctata cacactgata cttttattgg
     3961 agctcatggg attcgcgccc gagacgcgaa gctcacaaaa gtagtcgccc tcatccttgg
     4021 tggtgaagtt ggctagggta aggaccttga tatagggctg gttggagagg gtgacgcggg
     4081 agcggtacgt gtgctcgggt atcccaaggg tgcctgagag cacgtgcttc ctcttctctc
     4141 gggtcaggct gaactcatgc tggatggagt tatccttggt gttattctca tggcggcagt
     4201 ccaggcgaag gttttggttc accaggcagg ctgtcaggct ggtcaccttc tgccctcggg
     4261 acacctgcaa gactgagagc aggagagcga cgctgatggc tgggttcatg gtgcctcgag
     4321 gacggtgact gcagaaaaga cccatggttg tggccatatt atcatcgtgt ttttcaaagg
     4381 aaaaccacgt ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat
     4441 gtaaagcatg tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca
     4501 tccttcagcc ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc
     4561 caactcacaa cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc
     4621 ttttggccgc agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct
     4681 acagacgttg tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac
     4741 agaccttgca ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca
     4801 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
     4861 tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
     4921 gcggaattcg atatcaagct tatcgatacc gtcatcgaac cactttgtac aagaaagctg
     4981 ggtctagata tctcgagtgc ggccgcgaat tcggtaccgg atccagtcga ctcaagggca
     5041 gggattggag cacttgttgg ggcgttggct cctcttcttg cgaaactcaa attcatctac
     5101 ggtccacact gctcccttct cgctctccac tcgcacaaag cacttgtgca ggctcaggtt
     5161 gtggcggatg gcattcttcc aggtggcggg gtggtttctg aagtaggcga acatgcgagt
     5221 aaaccaatgg tagatttcat tgagtgtcct ctgcctctcc ggggcttcca ggatggccca
     5281 tcggataagg gtggcatagg tgaaaggggg ttgcatattg tggtacttga agtagtccat
     5341 gttgtggaag aactctggga aggaactatt gccatggctt ccccagaggt gcctccgcac
     5401 tgcaaacagg ccgccgtctg gagcctcccg aggagcagac caggccggga gcacactgcc
     5461 ctgagtactg gtggctacga tgcagcaaga gctcttgtcc attgaggcca cagatggagc
     5521 cttggccagc gccatcttcc cagccaggtg ggcctgcata gctcccagct tctccttttc
     5581 cagctccagc tgctgctcca gagactgcac cacttctctc tggaggaggc actgggcctt
     5641 gcctttctca tccaggagat gatctgcttg gcagtgcttg agaaactctt ctggctcctc
     5701 gaagaccttc tcacaaccag gccacttgca gactccattt gccagcagtg ggtaggatcc
     5761 ttggggtgca gccaaaaggt tgctgtcttt cctgggtgta cccgagcgtg ggaaggtgca
     5821 gagtagagct ggctccctgg acacccattc cagactggcc acattgatcc caggtggcag
     5881 gccaggccgg gccttgaggg agaagacccc agtggcagca gaaggtggtg ggaggctgat
     5941 catggctggg ttgtccagtg gacgcacttg gagcacaggg gtctgggcat gggcatccac
     6001 agtggagagc tgatgcatga agtgtggtct gtcctggaga agggcctgta ggtggggtga
     6061 gggacctagt cgggccccag acggtgccac catgactagg ggcactgtag gcagctgcag
     6121 ctgggatggt ggcagggggt tcaaggaaga agaggtgtgg gccccacttc gcaggtcccg
     6181 accttggaag ggtcccccag agcccctggt ccctagaagt tctgagccct tgggtgcagt
     6241 cttccagctt ggcaagactc ctggggatgg gccaagggcc aaggaaggag ccataggctt
     6301 ggctggccta gggttgggag atccagcgta atctggaaca tcgtatgggt acgccataga
     6361 tctaattccg gcgcctagag aaggagtgag ggctggataa agggaggatc gaggcggggt
     6421 cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag gcggaggctt
     6481 agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt cccaggtcac
     6541 gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct tgatcttaac
     6601 ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt taaaggttgg
     6661 ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg actggttgtg
     6721 agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag tggtaacagt
     6781 ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac acagaacgat
     6841 gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa gcaaaaattc
     6901 agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat atcctgacgg
     6961 ggtcggattc cacatcgact cccttcctca ggtcgggcca caaaaacggc ccccaaagtc
     7021 cctgggacgt ctcccagggt tgcggccggg tgttcagaac tcgtcagttc caccacgggt
     7081 ccgccagata cagagctagt tagctaacta gtacagacgc aggcgcaaac attagatgcc
     7141 ggcacaaaca cgcacaaaga cagagacaga cacgaaacga ccgctggcca gcttacctcc
     7201 cggcgggggg gtcggtggtc cctgggcagg ggtctccaaa tctcggtgga acctccaaat
     7261 gaaagacccc cgaggtgggc agtcaatcaa tctgaggaga ccctcccaag gatcagcgag
     7321 tccacgattc ggatgcaaac agcaagaggc tttattggga atacgggtac ccgggcgacg
     7381 cagtctatcg gaggactggc gcgccgagtg aggggttgtg ggctctttta ttgagctcgg
     7441 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
     7501 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
     7561 tgagggcggg accgcatctg gggaccatct gttcttggcc ctgagccggg gcagg
//
