LOCUS       sequence-457337-        6734 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of full length mouse FoxP3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6734)
  TITLE     Hur lab plasmids 2024
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA-HA FoxP3
FEATURES             Location/Qualifiers
     source          1..6734
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     356..375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     609..626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    743..764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(779..784,785..802,803..809)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    817..833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     822..844
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     841..857
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     841..857
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    906..1027
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(917..936)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     971..990
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1201..1995)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1312..1331)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1922..1941
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2062..2391)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2076..2211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2130..2149)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2376..2396
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2405..2833)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2515..2536)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2727..2746
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2879..3103
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3092..3109
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        3129..3147
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3129..3146
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(4501..4527)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4552..4571)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4553..4571)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4616..4819)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4645..4665)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4820..5199
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5371..5390
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5465..5569
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5570..5638,5639..6430)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5788..5807)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6601..6734,1..455)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
       61 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      121 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      181 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
      241 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
      301 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
      361 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
      421 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
      481 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
      541 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
      601 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
      661 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
      721 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
      781 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
      841 caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac ggtatacaga
      901 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      961 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1021 acaagttggg gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc
     1081 cggcgtcccg gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga
     1141 aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc
     1201 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
     1261 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
     1321 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
     1381 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
     1441 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
     1501 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
     1561 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
     1621 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
     1681 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
     1741 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
     1801 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
     1861 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
     1921 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
     1981 atcttgttca atcatgcgaa acgatcctca tcctgtctct tgatcagatc cgaaaatgga
     2041 tatacaagct cccgggagct ttttgcaaaa gcctaggcct ccaaaaaagc ctcctcacta
     2101 cttctggaat agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag
     2161 tcagccatgg ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt
     2221 taggggcggg actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     2281 tggggagcct ggggactttc cacacctggt tgctgactaa ttgagatgca tgctttgcat
     2341 acttctgcct gctggggagc ctggggactt tccacaccct aactgacaca cattccacag
     2401 aattaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2461 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     2521 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     2581 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     2641 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     2701 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     2761 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     2821 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
     2881 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
     2941 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
     3001 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
     3061 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcga
     3121 gctctagcat ttaggtgaca ctatagaata gggccctcta gatgcatgct cgagcggccg
     3181 ccagtgtgat ggatatctgc agaattctca agggcaggga ttggagcact tgttggggcg
     3241 ttggctcctc ttcttgcgaa actcaaattc atctacggtc cacactgctc ccttctcgct
     3301 ctccactcgc acaaagcact tgtgcaggct caggttgtgg cggatggcat tcttccaggt
     3361 ggcggggtgg tttctgaagt aggcgaacat gcgagtaaac caatggtaga tttcattgag
     3421 tgtcctctgc ctctccgggg cttccaggat ggcccatcgg ataagggtgg cataggtgaa
     3481 agggggtcgc atattgtggt acttgaagta gtccatgttg tggaagaact ctgggaagga
     3541 actattgcca tggcttcccc agaggtgcct ccgcactgca aacaggccgc cgtctggagc
     3601 ctcccgagga gcagaccagg ccgggagcac actgccctga gtactggtgg ctacgatgca
     3661 gcaagagctc ttgtccattg aggccacaga tggagccttg gccagcgcca tcttcccagc
     3721 caggtgggcc tgcatagctc ccagcttctc cttttccagc tccagctgct gctccagaga
     3781 ctgcaccact tctctctgga ggaggcactg ggccttgcct ttctcatcca ggagatgatc
     3841 tgcttggcag tgcttgagaa actcttctgg ctcctcgaag accttctcac aaccaggcca
     3901 cttgcagact ccatttgcca gcagtgggta ggatccttgg ggtgcagcca aaaggttgct
     3961 gtctttcctg ggtgtacccg agcgtgggaa ggtgcagagt agagctggct ccctggacac
     4021 ccattccaga ctggccacat tgatcccagg tggcaggcca ggccgggcct tgagggagaa
     4081 gaccccagtg gcagcagaag gtggtgggag gctgatcatg gctgggttgt ccagtggacg
     4141 cacttggagc acaggggtct gggcatgggc atccacagtg gagagctgat gcatgaagtg
     4201 tggtctgtcc tggagaaggg cctgtaggtg gggtgaggga cctagtcggg ccccagacgg
     4261 tgccaccatg actaggggca ctgtaggcag ctgcagctgg gatggtggca gggggttcaa
     4321 ggaagaagag gtgtgggccc cacttcgcag gtcccgacct tggaagggtc ccccagagcc
     4381 cctggtccct agaagttctg agcccttggg tgcagtcttc cagcttggca agactcctgg
     4441 ggatgggcca agggccaagg aaggagccat aggcttggct ggcctagggt tgggagatcc
     4501 agcgtaatct ggaacatcgt atgggtacgc catggtacca agcttgggtc tccctatagt
     4561 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     4621 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     4681 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     4741 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     4801 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     4861 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     4921 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     4981 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     5041 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     5101 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     5161 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     5221 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
     5281 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
     5341 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5401 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     5461 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5521 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5581 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5641 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5701 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5761 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5821 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5881 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5941 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6001 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6061 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6121 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6181 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6241 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6301 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6361 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6421 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6481 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6541 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6601 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6661 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6721 cagcagagcg caga
//
