LOCUS       sequence-457373-        5489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 12 CUG repeats in the context of human DMPK genomic 
            segment containing exons 11-15 with repeats inserted at site in exon
            15 that contains the repeats.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ho TH, Charlet-B N, Poulos MG, Singh G, Swanson MS, Cooper TA
  TITLE     Muscleblind proteins regulate alternative splicing.
  JOURNAL   EMBO J. 2004 Aug 4;23(15):3103-12. Epub 2004 Jul 15.
  PUBMED    15257297
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2004-08-4"; volume: "23(15)"; pages: "3103-12. Epub 2004 Jul 
            15"
COMMENT     Sequence Label: DT12A
FEATURES             Location/Qualifiers
     source          1..5489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     19..38
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        119..137
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     119..136
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(2655..2858)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2684..2704)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2859..3162
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(3261..3280)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3262..3280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3367..3384)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3538..4126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3618..3637)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4297..5088,5089..5157))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4920..4939
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5158..5262)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5330..5348
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5386..5408)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatggaca
       61 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
      121 ttaggtgaca ctatagaact cgagcgcagg cgcagtggaa ggaggatggc cgcgcgcgct
      181 gccagcccag ccccctcttc tcgacgctcg gtggcacagc tgggccacag ctgggcgggg
      241 gcggtgcctc cgggtggccc gctcgccctc ctattggccg gacgccaaag ccccgccccg
      301 tggcttttcc tcccccaacc ctgattcggc cgcttcgcat cccgctagct cctcccagac
      361 cttcggccgc ctccacacgc ctccggattg gcccgctgcg gagcctccgg cccacaacgc
      421 aaaccgcgga cactgtggag tccagagctt tgggcagatg gagggccttt tattcgcgag
      481 ggtcgggggt gggggtccta ggtggggaca gacaatagat accgaggaat gtcggggtct
      541 cagtgcatcc aaaacgtgga ttggggttgt tgggggtcct gtagcctgtc agcgagtcgg
      601 aggacgaggt caataaatat ccaaaccgcc gaagcgggcg gggccggctg gggctccgag
      661 agcagcgcaa gtgaggaggg gggcgcggga tccccgaaaa agcgggtttg gcaaaagcaa
      721 atttcccgag taagcaggca gagatcgcgc cagacgctcc ccagagcagg gcgtcatgca
      781 caagaaagct ttgcactttg cgaaccaacg ataggtgggg gtgcgtggag gatggaacac
      841 ggacggcccg gcttgctgcc ttcccaggcc tgcagtttgc ccatccacgt cagggcctca
      901 gcctggccga aagaaagaaa tggtctgtga tcccccgtcg agcagcagca gcagcagcag
      961 cagcagcagc agcagcagtc gacattcccg gctacaagga cccttcgagc cccgttcgcc
     1021 ggccgcggac ccggcccctc cctccccggc cgctaggggg cgggcccgga tcacaggact
     1081 ggagctgggc ggagacccac gctcggagcg gttgtgaact ggcaggcggt gggcgcggct
     1141 tctgtgccgt gccccgggca ctcagtcttc caacggggcc ccggagtcga agacagttct
     1201 agggttcagg gagcgcgggc ggctcctggg cggcgccaga ctgcggtgag ttggccggcg
     1261 tgggccacca acccaatgca gcccagggcg gcggcacgag acagaacaac ggcgaacagg
     1321 agcagggaaa gcgcctccga taggccaggc ctagggacct gcggggagag ggcgaggtca
     1381 acacccggca tgggcctctg attggctcct gggactcgcc ccgcctacgc ccataggtgg
     1441 gcccgcactc ttccctgcgc cccgcccccg ccccaacagc ctacagctgt tgttagtcca
     1501 ctcgcacgcc tcgaatcccg tccgaactcg tcattggctg cttcctagcg gcctgtgttg
     1561 attggctgcc cgaagatccg ccctcctgcc gtgggcccag ccccgcaaat gcgcagctaa
     1621 gcgggtggca cggggcgggt ggagcgcggg gcgcgacggc ggaggggggc gtgggcagcc
     1681 ggacgtaccc tggcagggag cagcaggtgg cggcggtgca tggggcctgg ccccaccagc
     1741 gggcactggc ccacagccac ggccgggggg ccatctagct ggagagagaa gggacaggtg
     1801 acccgatcgg agcccagccc agccctcagc ggtggggcga gagacagcga ggggaatcga
     1861 ggttggggag gttatctagg gagatcccgg agggaatctg gtgaggcctg aacggaggga
     1921 gatctggggc tgaataaagg gcttctgccc tctaaagtcg caaagacgta gggtgagccc
     1981 tatatctgga cggggagacc aggagccagg gaggggatct gcagaatggg cagcaggtct
     2041 gaggcagggg aaagagaggg gtcttacatg ggaaggtgga tccgtggccc ggggactggg
     2101 gacccccgtg acagctggaa ggagaagaaa gaggcatagg gcgcgtggag gggcgaagga
     2161 gggcggtggc gcggcgtgcc ccagcgtggg tcccttccct cctccaggtg tctatacacg
     2221 ccccgcggag cagacggccc acctcctccc ggtcctccgg ggaaggggac acatgaggga
     2281 ctcacctgtg gctccctctg cctgcagcaa ctccatccgc tcctgcaact gccggacgtg
     2341 tgcctctagg tcccggttcc gagcctctgc ctcgcgtagt tgactgtggg gaggtaagga
     2401 cggtgagtcc gtccgggccg gacgagaggg gctgccaagg gttgccaccg gcccgcatcc
     2461 cggccccggc cccggccccg atcccgacct ggcgaagttc tggttgtccg tgcggatggc
     2521 ctccatctcc cggctcaggc tctgccgggt gagcacctcc tcctccaggg cttcctggag
     2581 ctcccgcagc gtcacctcgg cctcagcctg atctgagtcc ggtagcgcta gcggatctga
     2641 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
     2701 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
     2761 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
     2821 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
     2881 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     2941 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     3001 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     3061 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     3121 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     3181 ctatgaacta atgaccccgt aattgattac tattagggta ccgagctcga attcatcgat
     3241 gatatcagat ctgccggtct ccctatagtg agtcgtatta atttcgataa gccaggttaa
     3301 cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     3361 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     3421 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3481 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3541 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3601 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3661 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3721 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3781 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3841 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3901 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3961 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4021 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4081 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4141 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4201 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     4261 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     4321 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     4381 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     4441 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     4501 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     4561 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     4621 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     4681 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     4741 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     4801 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     4861 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     4921 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     4981 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     5041 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     5101 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     5161 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5221 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5281 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     5341 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     5401 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     5461 gggcgcgtca gcgggtgttg gcgggtgtc
//
