LOCUS       sequence-457418-        3997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Notch reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Hansson EM, Teixeira AI, Gustafsson MV, Dohda T, Chapman G, Meletis 
            K, Muhr J, Lendahl U
  TITLE     Recording Notch signaling in real time.
  JOURNAL   Dev Neurosci. 2006;28(1-2):118-27.
  PUBMED    16508309
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev 
            Neurosci."; date: "2006"; volume: "28(1-2)"; pages: "118-27"
COMMENT     Sequence Label: 12XCSL-DsRedExpressDL
FEATURES             Location/Qualifiers
     source          1..3997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    55..76
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(91..96,97..114,115..121)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    129..145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     134..156
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     153..169
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     153..169
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    198..319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(209..228)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     263..282
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(576..1250)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(635..658)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(833..852)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1050..1070
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     1102..1120
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      1247..1256
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1264..1316
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     complement(1742..1759)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1742..1758)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1751..1773)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1954..1972)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2040..2144
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2145..2213,2214..3005)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2363..2382)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3176..3764
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3665..3684
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3918..3935
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
       61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
      181 ttcgagctcg gtacccttaa gatacattga tgagtttgga caaaccacaa ctagaatgca
      241 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      301 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      361 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      421 tgatctagag tcgcggccgc ctacacattg atcctagcag aagcacaggc tgcagggtga
      481 cggtccatcc cgctctcctg ggcacaagac atgggcagcg tgccatcatc ctgcgccgcc
      541 accgccggcg ggaagccatg gctgatatca ctagtcagga acaggtggtg gcggccctcg
      601 gcgcgctcgt actgctccac gatggtgtag tcctcgttgt gggaggtgat gtccagcttg
      661 gagtccacgt agtagtagcc gggcagctgc acgggcttct tggccatgta gatggacttg
      721 aactccacca ggtagtggcc gccgtccttc agcttcaggg ccttgtggat ctcgcccttc
      781 agcacgccgt cgcgggggta caggcgctcg gtggaggcct cccagcccat agtcttcttc
      841 tgcattacgg ggccgtcgga ggggaagttc acgccgatga acttcacctt gtagatgaag
      901 gagccgtcct gcagggagga gtcctgggtc acggtcacca cgccgccgtc ctcgaagttc
      961 atcacgcgct cccacttgaa gccctcgggg aaggacagct tcttgtagtc ggggatgtcg
     1021 gcggggtgct tcacgtacac cttggagccg tactggaact ggggggacag gatgtcccag
     1081 gcgaagggca gggggccgcc cttggtcacc ttcagcttgg cggtctgggt gccctcgtag
     1141 gggcggccct cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg
     1201 cgcaccttga agcgcatgaa ctccttgatg acgtcctcgg aggaggccat ggtggcgacc
     1261 ggtggatccc gggcccgcgg taccgtcgac tgcagaattc gaagcttgag ctcgagggat
     1321 tgcaagtaaa cacagttgtg tcaaaagcaa gtgtaagcag aagctgaagt gatctgcctt
     1381 ttatgcccaa gatgcataca gtggatctac tattttccca cggtgccctt ccgcccattt
     1441 tcccacgagt cgggatctac tattttccca cggtgccctt ccgcccattt tcccacgagt
     1501 cgggatctac tattttccca cggtgccctt ccgcccattt tcccatgagt cgggatctac
     1561 tattttccca cggtgccctt ccgcccattt tcccacgagt cgggatctac tattttccca
     1621 cggtgccctt ccgcccattt tcccacgagt cgggatctac tattttccca cggtgccctt
     1681 ccgcccattt tcccatgagt cgggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     1741 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1801 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1861 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     1921 ttacgcatct gtgcggtatt tcacaccgca taggcctcgt gatacgccta tttttatagg
     1981 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     2041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     2161 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     2221 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     2281 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     2341 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     2401 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     2461 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     2521 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     2581 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     2641 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     2701 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     2761 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     2821 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     2881 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2941 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     3001 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3061 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3121 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3301 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3361 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3421 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3481 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3541 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3601 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3661 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3721 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3781 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3841 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3901 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3961 aaccgcctct ccccgcgcgt tggccgattc attaatg
//
