LOCUS       sequence-457430-       11441 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A fusion of the Kinesin-1 motor domain (amino acids 1-560) to the v4
            SunTag peptide array.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 11441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-K560-24xGCN4_v4
FEATURES             Location/Qualifiers
     source          1..11441
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    497..631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(521..540)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     575..594
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1056..1076)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1206..1271
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(2405..2734)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2419..2554
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2719..2739
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(2809..2826)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2980..3568)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3060..3079)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3739..4530,4531..4599))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4362..4381
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4600..4704)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4772..4790
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4828..4850)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4950..4969
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5183..5202
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5492..5510
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5492..5509
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             5535..5768
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(5868..5924)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6012..6068)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6084..6140)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6156..6212)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6228..6284)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6300..6356)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6372..6428)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6444..6500)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6516..6572)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6588..6644)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6660..6716)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6732..6788)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6804..6860)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6876..6932)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(6948..7004)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7020..7076)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7092..7148)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7164..7220)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7236..7292)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7308..7364)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7380..7436)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7452..7508)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             complement(7524..7580)
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     promoter        complement(9327..9537)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9333..9468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9604..9721
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10006..10050)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10235..10468
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10965..11090
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(11137..11441,1..329)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg ctaggcggct
       61 gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca ggctcacagg
      121 gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg gctcaactgg
      181 tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc ctggtgtgta
      241 gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa ggatatcttg
      301 tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat atttaacaaa
      361 tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt aaatgaaacc
      421 acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga ttttggttag
      481 cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg gacaaaccac
      541 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
      601 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
      661 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
      721 tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt attaacccct
      781 ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat agcagacact
      841 ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt atgcctactt
      901 ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc tttttccttt
      961 gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg actcaaaaaa
     1021 cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt ttttggagga
     1081 gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc ttctgagcaa
     1141 aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt tccataggtt
     1201 ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac acttaaaaat
     1261 tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta tgtcacacca
     1321 cagaagtaag gttccttcac aaagatccgg ggcccactca taaatccagt tgccgccacg
     1381 gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc tcatcacaat
     1441 acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg cgccagatcc acatcagacg
     1501 gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact gcacaggcgg
     1561 ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt taaagcgggg
     1621 tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca gaatacttac
     1681 tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt gccaggcgtt
     1741 gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc cacggctgtt
     1801 caatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga cgaccagccg
     1861 gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc gcaaccgcag
     1921 taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg ccttctgcgc
     1981 gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata caaacggtat
     2041 cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtacagac cgccacggct
     2101 tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga gtttacgtgc
     2161 atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa gtgtcccagc
     2221 ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga gattatagag
     2281 atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg cactgcctct
     2341 ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc ttgtgtccaa
     2401 gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg aatagctcag
     2461 aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca tggggcggag
     2521 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     2581 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     2641 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     2701 agcctgggga ctttccacac cctaactgac acacattcca cagctgcatt aatgaatcgg
     2761 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2821 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2881 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2941 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3001 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3061 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3121 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3181 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     3241 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     3301 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3361 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3421 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3481 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3541 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3601 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3661 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3721 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3781 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3841 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3901 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3961 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4021 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4081 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4141 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     4201 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     4261 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     4321 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4381 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4441 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4501 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4561 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4621 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4681 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     4741 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     4801 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     4861 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     4921 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     4981 ccattcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     5041 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     5101 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     5161 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     5221 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
     5281 tgctgattgg ttcgctgacc atttccgggt gcgggacggc gttaccagaa actcagaagg
     5341 ttcgtccaac caaaccgact ctgacggcag tttacgagag agatgatagg gtctgcttca
     5401 gtaagccaga tgctacacaa ttaggcttgt acatattgtc gttagaacgc ggctacaatt
     5461 aatacataac cttatgtatc atacacatac gatttaggtg acactataga atacacggaa
     5521 ttaattctag atgctgctag agattttcca cactgactaa aagggtctga gggatctcta
     5581 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     5641 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     5701 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
     5761 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
     5821 cattggtctt aaaggtaccg gtgcggccgc tgacccgagc cagaaccctt tttaagtcgg
     5881 gctacttcat tctcgagatg ataatttttc gacagaagtt cctcaccact gccacttccc
     5941 ttttttaaac gtgcaacctc attttccaaa tgataatctt ttgagagcag ttcttcgcct
     6001 gatcctgatc ctttcttcaa acgtgcaact tcgttttcca ggtggtaatt cttactgagt
     6061 agttcctcac ctgatccgga ccccttttta agtcttgcaa cctcattttc aagatgatag
     6121 ttcttcgaca ggagttcctc cccactgccg gagcccttct ttagtcgagc cacctcgttc
     6181 tcgagatgat aattcttaga tagtaactct tccccgctcc cgctcccttt cttcaagcgc
     6241 gccacctcat tttccaagtg gtagttcttg ctcaagagct cttccccgct ccccgagcct
     6301 ttcttcaagc gggcgacttc attctctaag tggtagttct tcgataagag ctcctccccg
     6361 ctgccgctcc ctttcttgag ccttgccact tcgttctcca agtggtaatt tttgctcaat
     6421 aactcttcgc cgctcccact cccttttttg agcctggcga cttcattttc caagtggtag
     6481 tttttactca acaattcctc ccccgacccc gagcctttct tgagcctggc gacttcgttc
     6541 tccaaatgat aattcttgct gagcaattcc tcgccgctcc ccgacccttt cttcaatctc
     6601 gcgacctcat tctccaagtg gtagttcttg ctcaagagtt cctccccgct gccactgccc
     6661 tttttcaacc tggcgacctc attttcgagg tggtagttct tcgataagag ttcttcgccc
     6721 gagcccgagc ctttctttaa gcgcgcgact tcgttctcta aatgatagtt tttgctcaat
     6781 aactcctcgc ccgacccgct cccctttttc aacctcgcga cctcattttc caagtggtag
     6841 tttttcgaga gcaactcctc gcctgagcct gagccctttt tgagcctagc aacttcgttc
     6901 tcaagatgat aatttttcga tagcaactct tcgccagaac cactcccctt ttttagtctc
     6961 gctacctcat tttccagatg ataattttta ctcagtaatt cttcacccga ccctgaacct
     7021 ttctttaatc gagctacttc gttttcgagg tggtaattct ttgaaagtag ttcctcacca
     7081 ctgcccgatc ccttttttaa tcgagcgacc tcattttcaa gatgatagtt cttcgagagc
     7141 agttcctctc ctgagccgga acccttcttc aaacgcgcta cctcgttctc aagatgataa
     7201 ttcttggata gtagctcttc accactgcca cttccctttt ttaagcgtgc cacctcattt
     7261 tcgaggtggt agtttttaga taatagctct tctccagaac cactgccctt ttttagccga
     7321 gccacttcgt tttcgagatg ataatttttc gagagtaact cctcacccga tccggaccct
     7381 ttctttagtc gtgctacttc gttctccaaa tgataatttt tgctcagcaa ctcctcccca
     7441 cttccgctcc cctttttcag tctagctacc tcattttcca ggtggtaatt ctttgaaagc
     7501 agttcttctc cactgccaga acctttctta agacgagcca cttcgttctc aagatgataa
     7561 ttcttgctca aaagttcttc tcctccagaa cctccacctc cagatccacc cggaccgttg
     7621 gatccggtac cttttagtaa agatgccatc atctcagctg ctcgtttttt ctggtggttg
     7681 gtcatttcct taagtttctg aagctcagca tctatactcg ctaaagttgc cgattcctga
     7741 ttcaattcat cactaagcaa ttcatattcc ttagttttgt cttcaacttc ctgagacttc
     7801 tgatcataat tgacagcaag ttcttctagg gcctgtaaaa cttctttcac ttcttcttta
     7861 gaggcatcat tttctgcttg aaggcgattc agctcagctt gcatattgtc ttgatccctt
     7921 ctggtagatg ccaaaagctc ctcctgatcc aacatttgcg tcttcagctt ctctaccagt
     7981 tgactttgct ggttaatttc ttcatccttg tcatcaagct gtttgtataa tttagcaatt
     8041 tcttcttcac actttcttct ttcagcatca gtaaaatttc ctataactcc aattgcggtt
     8101 gctggtttat cattggtaag agtaatatct ttatccactg tgaaagcttc caagttggct
     8161 ttctctttgt caaactgttc atcaataggc accgtctccc cattacgcca tctgttgagc
     8221 tcattttcaa gccactgaat agtgttccgc aggatcttat ttttttcttt ttctttttca
     8281 tacttctttt tccactgttc tgcagttaac tccacattga cacaaactgt gttcttaatt
     8341 gttttggccc tttggccaaa taagagtgta gattttgttt cagactcatt gtatgatgat
     8401 ggagagcagc aaattacaat agtggttcta cagttgccac ctaatgaatc ttgaaggatt
     8461 cttgtcattt tactatctcg atatggaaca taagtactac cctcagccaa agcagaaata
     8521 acatttccaa gagcagaaag tgacttgttg atgtttttag cttcatccag cacagcacct
     8581 tcagctccag ttttactaac cttttcacta ccagctaaat caactagata aagttttcca
     8641 ctcagctttt gttccgtttg tgtgttctct tgtttgacat taataagaaa tatactgtga
     8701 ctcctagagc tatgttcatt catatttgta actgctacat gtctgttgga ttttccttca
     8761 tctatggtat ccataacttc atctggacta catacaaaac gctctgtgca cccctttaca
     8821 tagggaactc ggtttttgtc ttcatgaact gaaaggttgg tctttgaaac atctaacagg
     8881 tcccttatct tatccaaata tatttcaaaa tatgaaacct taatatgaaa ttccaaattt
     8941 tcatccatgg agtaaatata attaaaaata tcttgcacta ttcttggaat aattcccatg
     9001 ccttctggat catgaagttt accctccatt gtgtgtgtct tcccagagga tgtttggccg
     9061 tatgcaaata ttgttccatt atatccttca agtacatctt taacaatctt ctttgcacag
     9121 tcattataca cttgctcttg agatgtgctt gactggaaca cccgatcaaa tgcataaggc
     9181 ttggacgcga tcacgaccgt gtcttctccc tgaaacttgg cgatgtactt gtcgccgcgg
     9241 ttcacttcag actcgttgag aggtctgaag cgacacatca ctttgatgtt gcactcggcc
     9301 aggtccgcca tcatggtggc acgcgtagcc taggcctcca aaaaagcctc ctcactactt
     9361 ctggaatagc tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca
     9421 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
     9481 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgcctg
     9541 aattcgatat caagcttatc gatatcgata aactggatct ctgctgtccc tgtaataaac
     9601 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     9661 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     9721 attgtggatg aatactgcca tttggcgata ccgtcgagat ccgttcacta atcgaatgga
     9781 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
     9841 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     9901 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     9961 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
    10021 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
    10081 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
    10141 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
    10201 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
    10261 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
    10321 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
    10381 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
    10441 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
    10501 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
    10561 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
    10621 ccggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
    10681 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
    10741 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
    10801 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
    10861 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
    10921 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
    10981 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
    11041 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
    11101 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
    11161 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
    11221 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
    11281 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa agcagctgct
    11341 tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg cctccctgga
    11401 aagtccccag cggaaagtcc cttgtagcaa gctcgatatc a
//
