LOCUS       sequence-457466-        8353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gram-Positive Bacterial Shuttle Vector for Nisin-Controlled 
            Inducible Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Bryan EM, Bae T, Kleerebezem M, Dunny GM
  TITLE     Improved vectors for nisin-controlled expression in gram-positive 
            bacteria.
  JOURNAL   Plasmid. 2000 Sep;44(2):183-90.
  PUBMED    10964628
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2000-09"; volume: "44(2)"; pages: "183-90"
COMMENT     Sequence Label: pMSP3535
FEATURES             Location/Qualifiers
     source          1..8353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      367..955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     856..875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1109..1126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1213..1232
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1213..1231
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
       61 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
      121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
      181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
      241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
      301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
      361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
      421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
      481 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
      541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
      601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
      661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
      721 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
      781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
      841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
      901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
      961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     1141 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca ggttaacctg
     1201 gcttatcgaa attaatacga ctcactatag ggagaccggc ctcgagtcta gaagcgctgt
     1261 cgacgagctc gcatgcgaat tcctgcagcc cgggactagt ggtaccggat ccatgcagag
     1321 tctcctgttt tacaaccggg tgtacatagc gaaatacttg taatgcgtgg tgatgcacct
     1381 gaatctttct tcgaaacaga taccaaatcc aagctaaaat cttttgtact cattttgagt
     1441 gcctccttat aatttatttt gtagttcctt cgaacgaaat cattgtatct aacaaacttc
     1501 agaatttaat cagagccgtt tattatgctc gcgttatcga caataatatt attaccaata
     1561 ctttctcaag atagaattaa gactgtttta gatttgttaa tgtttctatt gtcagtatag
     1621 ttataagact agatctgatc cgtagcggtt ttcaaaattt gcaaccagga atgaattact
     1681 atccctttta tcaagaagcg cacaaaaaga aaaacgaaat gatacaccaa tcagtgcaaa
     1741 aaaagatata atgggagata agacggttcg tgttcgtgct gacttgcacc atatcataaa
     1801 aatcgaaaca gcaaagaatg gcggaaacgt aaaagaagtt atggaaataa gacttagaag
     1861 caaacttaag agtgtgttga tagtgcagta tcttaaaatt ttgtataata ggaattgaag
     1921 ttaaattaga tgctaaaaat ttgtaattaa gaaggagtga ttacatgaac aaaaatataa
     1981 aatattctca aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga
     2041 atttaaaaga aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga
     2101 aactggctaa aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact
     2161 tatcgtcaga aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa gatattctac
     2221 agtttcaatt ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa
     2281 gcacacaaat tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg
     2341 ttgaagaagg attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc
     2401 acactcaagt ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac
     2461 caaaagtaaa cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat
     2521 attggaagct atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt
     2581 ttactaaaaa tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg
     2641 ttacttatga gcaagtattg tctattttta atagttatct attatttaac gggaggaaat
     2701 aattctatga gtcgcttttg taaatttgga aagttacacg ttactaaagg gaatgtagat
     2761 aaattattag gtatactact gacagcttcc aaggagctaa agaggtccct agcgcttaga
     2821 atcgctttag gaaacacgat ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt
     2881 ggtgaaatta cctacttttg gaataatcac aaatcacaag tgattaatca caaatcacaa
     2941 gtgattaatc acttgtttat taagatatta aaagctataa tttaaataaa gcgtgaattt
     3001 tattacacaa aaagaggggg gagaaacttg gaactagcat ttagagaaag cttaaaaaag
     3061 atgagaggta ccaaatcaaa agaaaaattc tcccaagaat tagaaatgag tagatcaaat
     3121 tattcacgaa tagaatcagg aaaatcagat ccaaccataa aaacactaga acaaattgca
     3181 aagttaacta actcaacgct agtagtggat ttaatcccaa atgagccaac agaaccagaa
     3241 ccagaaacag aatcagaaca agtaacattg gatttagaaa tggaagaaga aaaaagcaat
     3301 gacttcgtgt gaataatgca cgaaatcgtt gcttattttt tttaaaagcg gtatactaga
     3361 tataacgaaa caacgaactg aatagaaacg aaaaaagagc catgacacat ttataaaatg
     3421 tttgacgaca ttttataaat gcatagcccg ataagattgc caaaccaacg cttatcagtt
     3481 agtcagatga actcttccct cgtaagaagt tatttaatta actttgtttg aagacggtat
     3541 ataaccgtac tatcattata tagggaaatc agagagtttt caagtatcta agctactgaa
     3601 tttaagaatt gttaagcaat caatcggaaa tcgtttgatt gctttttttg tattcattta
     3661 tagaaggtgg agtttgtatg aatcatgatg aatgtaaaac ttatataaaa aatagtttat
     3721 tggagataag aaaattagca aatatctata cactagaaac gtttaagaaa gagttagaaa
     3781 agagaaatat ctacttagaa acaaaatcag ataagtattt ttcttcggag ggggaagatt
     3841 atatatataa gttaatagaa aataacaaaa taatttattc gattagtgga aaaaaattga
     3901 cttataaagg aaaaaaatct ttttcaaaac atgcaatatt gaaacagttg aatgaaaaag
     3961 caaaccaagt taattaaaca acctatttta taggatttat aggaaaggag aacagctgaa
     4021 tgaatatccc ttttgttgta gaaactgtgc ttcatgacgg cttgttaaag tacaaattta
     4081 aaaatagtaa aattcgctca atcactacca agccaggtaa aagcaaaggg gccatttttg
     4141 cgtatcgctc aaaatcaagc atgattggcg gtcgtggtgt tgttctgact tccgaggaag
     4201 cgattcaaga aaatcaagat acatttacac attggacacc caacgtttat cgttatggaa
     4261 cgtatgcaga cgaaaaccgt tcatacacga aaggacattc tgaaaacaat ttaagacaaa
     4321 tcaatacctt ctttattgat tttgatattc acacggcaaa agaaactatt tcagcaagcg
     4381 atattttaac aaccgctatt gatttaggtt ttatgcctac tatgattatc aaatctgata
     4441 aaggttatca agcatatttt gttttagaaa cgccagtcta tgtgacttca aaatcagaat
     4501 ttaaatctgt caaagcagcc aaaataattt cgcaaaatat ccgagaatat tttggaaagt
     4561 ctttgccagt tgatctaacg tgtaatcatt ttggtattgc tcgcatacca agaacggaca
     4621 atgtagaatt ttttgatcct aattaccgtt attctttcaa agaatggcaa gattggtctt
     4681 tcaaacaaac agataataag ggccttactc gttcaagtct aacggtttta agcggtacag
     4741 aaggcaaaaa acaagtagat gaaccctggt ttaatctctt attgcacgaa acgaaatttt
     4801 caggagaaaa gggtttaata gggcgtaata acgtcatgtt taccctctct ttagcctact
     4861 ttagttcagg ctattcaatc gaaacgtgcg aatataatat gtttgagttt aataatcgat
     4921 tagatcaacc cttagaagaa aaagaagtaa tcaaaattgt tagaagtgcc tattcagaaa
     4981 actatcaagg ggctaatagg gaatacatta ccattctttg caaagcttgg gtatcaagtg
     5041 atttaaccag taaagattta tttgtccgtc aagggtggtt taaattcaag aaaaaaagaa
     5101 gcgaacgtca acgtgttcat ttgtcagaat ggaaagaaga tttaatggct tatattagcg
     5161 aaaaaagcga tgtatacaag ccttatttag tgacgaccaa aaaagagatt agagaagtgc
     5221 taggcattcc tgaacggaca ttagataaat tgctgaaggt actgaaggcg aatcaggaaa
     5281 ttttctttaa gatcaaacca ggaagaaatg gtggcattca acttgctagt gttaaatcat
     5341 tgttgctatc gatcattaaa gtaaaaaaag aagaaaaaga aagctatata aaggcgctga
     5401 caaattcttt tgacttagag catacattca ttcaagagac tttaaacaag ctagcagaac
     5461 gccctaaaac ggacacacaa ctcgatttgt ttagctatga tacaggctga aaataaaacc
     5521 cgcactatgc cattacattt atatctatga tacgtgtttg ttttttcttt gctgtttagc
     5581 gaatgattag cagaaatata cagagtaaga ttttaattaa ttattagggg gagaaggaga
     5641 gagtagcccg gaaactttta gttggcttgg actgaacgaa gtgagggaaa ggctactaaa
     5701 acgtcgaggg gcagtgagag cgaagcgaac acttgatttt ttaattttct atcttttata
     5761 ggtcattaga gtatacttat ttgtcctata aactatttag cagcataata gatttattga
     5821 ataggtcatt taagttgagc atattagagg aggaaaatct tggagaaata tttgaagaac
     5881 ccgattacat ggattggatt agttcttgtg gttacgtggt ttttaactaa aagtagtgaa
     5941 tttttgattt ttggtgtgtg tgtcttgttg ttagtatttg ctagtcaaag tgattaaata
     6001 gaattctcat gtttgacagc ttatcatcgg agctccgatg ataagctgtc aaacatgaga
     6061 attcccggct ttaggtatag tgtgtatctc aatccttggt atattgaaaa gaaagactaa
     6121 aaattgatag attatatttc ttcagaatga atggtataat gaagtaatga gtactaaaca
     6181 atcggaggta aagtggtgta taaaatttta atagttgatg atgatcagga aattttaaaa
     6241 ttaatgaaga cagcattaga aatgagaaac tatgaagttg cgacgcatca aaacatttca
     6301 cttcccttgg atattactga ttttcaggga tttgatttga ttttgttaga tatcatgatg
     6361 tcaaatattg aagggacaga aatttgtaaa aggattcgca gagaaatatc aactccaatt
     6421 atctttgtta gtgcgaaaga tacagaagag gatattataa acggcttagg tattggtggg
     6481 gatgactata ttactaagcc ttttagcctt aaacagttgg ttgcaaaagt ggaagcaaat
     6541 ataaagcgag aggaacgcaa taaacatgca gttcatgttt tttcagagat tcgtagagat
     6601 ttaggaccaa ttacatttta tttagaagaa aggcgagtct gtgtcaatgg tcaaacaatt
     6661 ccactgactt gtcgcgaata cgatattctt gaattactat cacaacgaac ttctaaagtt
     6721 tatacgagag aggatattta tgatgacgta tatgatgaat attctaatgc actttttcgg
     6781 tcaatctcgg aatatattta tcagattagg agtaagtttg caccatacga tattaatccg
     6841 ataaaaacgg ttcggggact tgggtatcag tggcatgggt aaaaaatatt caatgcgtcg
     6901 acggatatgg caagctgtca ttgaaattat cataggtact tgtctactta tcctgttgtt
     6961 actgggcttg actttctttc tacgacaaat tggacaaatc agtggttcag aaactattcg
     7021 tttatcttta gattcagata atttaactat ttctgatatc gaacgtgata tgaaacacta
     7081 cccatatgat tatattattt ttgacaatga tacaagtaaa attttgggag gacattatgt
     7141 caagtcggat gtacctagtt ttgtagcttc aaaacagtct tcacataata ttacagaagg
     7201 agaaattact tatacttatt caagcaataa gcatttttca gttgttttaa gacaaaacag
     7261 tatgcctgaa tttacaaatc atacgcttcg ttcaatttct tataatcaat ttacttacct
     7321 tttctttttt cttggtgaaa taatactcat tattttttct gtctatcatc tcattagaga
     7381 attttctaag aattttcaag ccgttcaaaa gattgcattg aagatggggg aaataactac
     7441 ttttcctgaa caagaggaat caaaaattat tgaatttgat caggttctga ataacttata
     7501 ttcgaaaagt aaggagttag ctttccttat tgaagcggag cgtcatgaaa aacatgattt
     7561 atccttccag gttgctgcac tttcacatga tgttaagaca cctttaacag tattaaaagg
     7621 aaatattgaa ctgctagaga tgactgaagt aaatgaacaa caagctgatt ttattgagtc
     7681 aatgaaaaat agtttgactg tttttgacaa gtattttaac acaatgatta gttatacaaa
     7741 acttttgaat gatgaaaatg attacaaagc gataatctcc ctggaggatt ttttgataga
     7801 tttatcagtt gagttggaag agttgtcaac aacttatcaa gtggattatc agctagttaa
     7861 aaaaacagat ttaaccactt tttacggaaa tacattagct ttaagtcgag cacttatcaa
     7921 tatctttgtt aatgcctgtc agtatgctaa agagggtgaa aaaatagtcg gtttgagtat
     7981 ttatgatgat gaaaaatatc tctattttga aatctggaat aatggtcatc ctttttctga
     8041 acaagcaaaa aaaaatgctg gaaaactatt tttcacagaa gatactggac gtagtgggaa
     8101 acactatggg attggactat cttttgctca aggtgtagct ttaaaacatc aaggaaactt
     8161 aattctcagt aatcctcaaa aaggtggggc agaagttatc ctaaaaataa aaaagtaatt
     8221 tagtaatctc taaggattac tttttttgtt tcggcaaact attaactggc gaactactta
     8281 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8341 ttctgcgctc ggc
//
