LOCUS       sequence-457471-       10594 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 24xgp41 peptide array (MoonTag peptide array), followed by
            kif18b gene and 24 repeats of PP7 hairpins in 3' UTR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10594)
  AUTHORS   Boersma S, Khuperkar D, Verhagen BMP, Sonneveld S, Grimm JB, Lavis 
            LD, Tanenbaum ME
  TITLE     Multi-Color Single-Molecule Imaging Uncovers Extensive Heterogeneity
            in mRNA Decoding.
  JOURNAL   Cell. 2019 Jun 5. pii: S0092-8674(19)30499-4. doi: 
            10.1016/j.cell.2019.05.001.
  PUBMED    31178119
REFERENCE   2  (bases 1 to 10594)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2019 
            Jun 5. pii: S0092-8674(19)30499-4. doi: 10.1016/j.cell.2019.05.001."
COMMENT     Sequence Label: 24xMoonTag-kif18b-24xPP7 (MoonTag translation
            ...
FEATURES             Location/Qualifiers
     source          1..10594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    820..838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    841..859
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     869..893
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1002..1046
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1062..1106
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1122..1166
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1182..1226
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1242..1286
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1302..1346
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1362..1406
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1422..1466
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1482..1526
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1542..1586
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1602..1646
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1662..1706
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1782..1826
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1842..1886
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1902..1946
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             1962..2006
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2022..2066
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2082..2126
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2142..2186
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2202..2246
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2262..2306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2322..2366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2382..2426
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     complement(6602..6619)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6608..6832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6878..7306
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6965..6984)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7175..7196
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7315..7335)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7320..7649
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7500..7635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7562..7581
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7697..7744
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7763..8137
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8267..8388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8304..8323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8358..8377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8437..8453)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8437..8453)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8450..8472)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8461..8477
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8485..8491,8492..8509,8510..8515))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8530..8551
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8668..8685)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8839..9427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8919..8938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9598..10389,10390..10458))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10221..10240
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10459..10563)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaaactaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc
      841 tccctatcag tgatagagat cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga
      901 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgga
      961 ctctagcgtt taaacttaag cttgccacca tgcgtacggg caagaatgag caggaactgc
     1021 tggaactgga caaatgggca agcctgggga gcggctccgg aaagaacgag caggaactgc
     1081 tggaactgga caagtgggct tctctgggct ccgggtccgg gaaaaacgaa caggagctgc
     1141 tggagctgga taagtgggcc agcctgggct ccgggtccgg gaagaatgaa caggaactgc
     1201 tggaactgga taaatgggca tctctgggca gcgggtccgg aaagaatgaa caggagctgc
     1261 tggaactgga caagtgggca tccctgggga gcgggtccgg aaagaatgaa caggaactgc
     1321 tggaactgga taagtgggca tccctgggaa gcggctccgg aaaaaacgag caggagctgc
     1381 tggagctgga caagtgggcc tctctgggct ccggctccgg gaagaatgaa caggagctgc
     1441 tggaactgga taagtgggca tctctggggt ccggaagcgg taaaaacgag caggaactgc
     1501 tggaactgga caagtgggca agcctggggt ctggctccgg caaaaacgaa caggaactgc
     1561 tggaactgga caaatgggcc agcctgggct ccgggtccgg gaaaaatgag caggaactgc
     1621 tggaactgga taaatgggcg agcctgggca gcgggtccgg aaaaaacgaa caggagctgc
     1681 tggagctgga caaatgggcg agtctgggct ccggaagcgg caagaatgaa caggaactgc
     1741 tggagctgga caaatgggcc agcctgggca gcggctccgg aaagaatgag caggaactgc
     1801 tggagctgga caaatgggca tctctgggct ccggctccgg gaaaaacgag caggaactgc
     1861 tggagctgga caagtgggct tccctgggca gcggctccgg aaaaaatgaa caggaactgc
     1921 tggaactgga caagtgggcc agcctggggt ccgggtctgg gaagaacgag caggaactgc
     1981 tggagctgga caagtgggca tctctggggt ctggctctgg caagaacgag caggaactgc
     2041 tggaactgga caagtgggcg agcctgggca gcgggtctgg caagaatgaa caggagctgc
     2101 tggaactgga caaatgggcc agcctggggt ctgggtctgg aaagaatgag caggaactgc
     2161 tggagctgga caagtgggca tctctgggct ctgggtctgg caagaacgag caggagctgc
     2221 tggagctgga taagtgggca tccctggggt ccgggtccgg caagaatgaa caggagctgc
     2281 tggagctgga taaatgggca tccctgggct ctgggtctgg gaagaatgag caggagctgc
     2341 tggaactgga taagtgggcg agcctgggca gcgggtctgg caagaatgag caggaactgc
     2401 tggagctgga caagtgggct tctctgcacc ggtcagcagt ggaggacagc acgctgcaag
     2461 tagtggtacg ggtgcggccc cccacccctc gggagctgga cagtcagcgg cggccagtgg
     2521 ttcaggtggt ggacgagcgg gtgctggtgt ttaaccctga ggagcccgat ggagggttcc
     2581 ctggcctgaa atggggtggc acccatgatg gccccaagaa gaagggcaaa gacctgacgt
     2641 ttgtctttga ccgggtcttt ggcgaggcgg ccacccaaca ggacgtgttc cagcacacca
     2701 cgcacagcgt cctggacagc ttcctccagg gctacaactg ctcagtgttt gcctacgggg
     2761 ccaccggggc tgggaagaca cacaccatgc tgggaaggga gggggacccc ggcatcatgt
     2821 acctgaccac cgtggaactg tacaggcgcc tggaggcccg ccagcaggag aagcacttcg
     2881 aggtgctcat cagctaccag gaggtgtata atgaacagat ccatgacctc ctggagccca
     2941 aggggcccct tgccatccgc gaggaccccg acaagggggt ggtggtgcaa ggactttctt
     3001 tccaccagcc agcctcagcc gagcagctgc tggagatact gaccaggggg aaccgtaacc
     3061 gcacgcagca ccccactgat gccaacgcga cttcctcccg ctcccatgcc atcttccaga
     3121 tctttgtgaa gcagcaggac cgggttccag gactgaccca ggctgtccag gtggccaaga
     3181 tgagcctgat tgacctggct ggctcagagc gggcatccag cacccatgcg aagggggagc
     3241 ggctgcggga gggggccaac atcaaccgct ctctgctggc gctcatcaac gtcctcaatg
     3301 ccttggccga tgcaaagggc cgcaagaccg ctgtgcccta cgcggacagc gcactgaccc
     3361 gcctgctcaa agactccctc gggggcaact gccgcacagt gatgatcgct gccatcagcc
     3421 cctccagcct gacctacgag gacacgtata ataccctcaa atatgccgac cgggccaagg
     3481 agatcaggct ctcgctgaag agcaatgtga ccagcctgga ctgtcacatc agccagtatg
     3541 ctaccatctg ccaacagctc caggctgagg tagccgctct gaggaagaag ctccaagtgt
     3601 atgagggggg aggccagccc ccaccacagg acctcccagg atctcccaag tcgggaccac
     3661 caccagaaca ccttcccagc tcccccttgc caccccaccc tcccagccag ccctgcaccc
     3721 cagagctccc tgcagggcct agagcccttc aagaggagag tctggggatg gaggcccagg
     3781 tggagagggc catggaaggg aactcttcag accaggagca gtccccagag gatgaggatg
     3841 aaggcccagc tgaggaggtt ccaacccaga tgccagagca gaaccccaca catgcactgc
     3901 cagagtcccc tcgcctgacc ctgcagccca agccagtcgt gggccacttc tcagcacggg
     3961 aactggatgg ggaccgttct aagcggttgg ccctaaaggt gctgtgcgtt gcccagcggc
     4021 agtactccct gctccaagca gccaacctcc tgacgcccga catgatcaca gagtttgaga
     4081 ccctacagca gctggtgcaa gaggaaaaaa ttgagcctgg ggcagaggcc ttgaggactt
     4141 caggcctggc caggggggca cctctggctc aggagctgtg ttcagagtca aagcctccag
     4201 gatacactgg ccctgtgacc cggactatgg cgaggcgact gagtggcccc ctgcacaccc
     4261 tgggaatccc gcctggaccc aactgcaccc cagcccaggg gtcccgatgg cccatggaga
     4321 agaagaggag gagaccaagc gccttggagg cagacagtcc catggcccca aagcggggca
     4381 ccaagcgcca gcgccagtcc ttcctgccct gcctaaggag agggtctctg cctgacaccc
     4441 aaccttcaca ggggcccagc acccccaaag gagaaagggc ctcctccccc tgccattccc
     4501 ctcgcgtttg cccagccaca gtcatcaaaa gccgggtgcc cctgggccct tccgccatgc
     4561 agaactgctc caccccgctg gctctgccca ctcgagacct caatgccacc tttgatctct
     4621 ctgaggagcc tccctcaaag cccagtttcc atgaatgcat tggctgggac aaaatacccc
     4681 aggagctgag caggctggac cagcccttca tccccagggc acctgtgccc ctgttcacca
     4741 tgaagggccc caagccaaca tcttccctcc ctgggacctc tgcctgcaag aagaagcgcg
     4801 ttgcgagttc ctcagtctcc catggccgca gccgcatcgc ccgcctcccc agcagcactt
     4861 tgaagaggcc agctgggccc cttgtactcc cagagctgcc cttgagtccc ctgtgcccta
     4921 gcaaccggag gaatggaaag gacctcatca gggtggggag agcactctca gcagggaacg
     4981 gcgtcaccaa ggtgtccgat atctaacgac acgattcgaa gatcgcatcg ctagcccggt
     5041 tgtacaggta ccggatccta aggtacctaa ttgcctagaa aggagcagac gatatggcgt
     5101 cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg gctgcagtat
     5161 tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga cgatatggcg
     5221 tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct ggctgcagta
     5281 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaggagcag acgatatggc
     5341 gtcgctccct gcaggtcgac tctagaaacc agcagagcat atgggctcgc tggctgcagt
     5401 attcccgggt tcattagatc ctaaggtacc taattgccta gaaaggagca gacgatatgg
     5461 cgtcgctccc tgcaggtcga ctctagaaac cagcagagca tatgggctcg ctggctgcag
     5521 tattcccggg ttcattagat cctaaggtac ctaattgcct agaaaggagc agacgatatg
     5581 gcgtcgctcc ctgcaggtcg actctagaaa ccagcagagc atatgggctc gctggctgca
     5641 gtattcccgg gttcattaga tcctaaggta cctaattgcc tagaaaggag cagacgatat
     5701 ggcgtcgctc cctgcaggtc gactctagaa accagcagag catatgggct cgctggctgc
     5761 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaagga gcagacgata
     5821 tggcgtcgct ccctgcaggt cgactctaga aaccagcaga gcatatgggc tcgctggctg
     5881 cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaagg agcagacgat
     5941 atggcgtcgc tccctgcagg tcgactctag aaaccagcag agcatatggg ctcgctggct
     6001 gcagtattcc cgggttcatt agatcctaag gtacctaatt gcctagaaag gagcagacga
     6061 tatggcgtcg ctccctgcag gtcgactcta gaaaccagca gagcatatgg gctcgctggc
     6121 tgcagtattc ccgggttcat tagatcctaa ggtacctaat tgcctagaaa ggagcagacg
     6181 atatggcgtc gctccctgca ggtcgactct agaaaccagc agagcatatg ggctcgctgg
     6241 ctgcagtatt cccgggttca ttagatccta aggtacctaa ttgcctagaa aggagcagac
     6301 gatatggcgt cgctccctgc aggtcgactc tagaaaccag cagagcatat gggctcgctg
     6361 gctgcagtat tcccgggttc attagatcct aaggtaccta attgcctaga aaggagcaga
     6421 cgatatggcg tcgctccctg caggtcgact ctagaaacca gcagagcata tgggctcgct
     6481 ggctgcagta ttcccgggtt cattagatct cgcgaagggc gaattcacga ggcgcgcccg
     6541 gatcgcgatc gcaatcttaa ttaactcgag tctagagggc ccgtttaaac ccgctgatca
     6601 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     6661 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     6721 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     6781 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     6841 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     6901 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6961 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7021 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7081 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7141 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7201 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7261 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7321 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7381 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7441 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     7501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7561 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7621 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7681 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     7741 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     7801 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     7861 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     7921 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     7981 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8041 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8101 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8161 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8221 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8401 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8461 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8521 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8581 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8641 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8701 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8761 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8821 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8881 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8941 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9001 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9061 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9121 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9181 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9241 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9301 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9361 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9421 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9481 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9541 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9601 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9661 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9721 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9781 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9841 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9901 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9961 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10021 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10081 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10141 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10201 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10261 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10321 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10381 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10441 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10501 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10561 gcgcacattt ccccgaaaag tgccacctga cgtc
//
