LOCUS       sequence-457491-         357 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p65 activation domain coupled to catalytically inactive dCas9 for 
            localization; contains GFP for labeling and as a spacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 357)
  TITLE     HP1 binding creates a local barrier against transcription activation
            and persists during chromatin decondensation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pdCas9-GFP-p65
FEATURES             Location/Qualifiers
     source          1..357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..357
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
ORIGIN
        1 cctacacagg ccggcgaggg cacactgtct gaagctctgc tgcagctgca gttcgacgac
       61 gaggatctgg gagccctgct gggaaacagc accgatcctg ccgtgttcac cgacctggcc
      121 agcgtggaca acagcgagtt ccagcagctg ctgaaccagg gcatccctgt ggcccctcac
      181 accaccgagc ccatgctgat ggaatacccc gaggccatca cccggctcgt gacaggcgct
      241 cagaggcctc ctgatccagc tcctgcccct ctgggagcac caggcctgcc taatggactg
      301 ctgtctggcg acgaggactt cagctctatc gccgatatgg atttctcagc cttgctg
//
