LOCUS       sequence-457498-        1569 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VPR activation domain coupled to catalytically inactive dCas9 for 
            localization; contains tdTomato for labeling and as a spacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1569)
  TITLE     HP1 binding creates a local barrier against transcription activation
            and persists during chromatin decondensation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pdCas9-tdTomato-VPR
FEATURES             Location/Qualifiers
     source          1..1569
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..151
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             176..196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             626..982
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             1001..1567
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSL"
ORIGIN
        1 cgacgcattg gacgattttg atctggatat gctgggaagt gacgccctcg atgattttga
       61 ccttgacatg cttggttcgg atgcccttga tgactttgac ctcgacatgc tcggcagtga
      121 cgcccttgat gatttcgacc tggacatgct gattaactct agaagttccg gatctccgaa
      181 aaagaaacgc aaagttggta gccagtacct gcccgacacc gacgaccggc accggatcga
      241 ggaaaagcgg aagcggacct acgagacatt caagagcatc atgaagaagt cccccttcag
      301 cggccccacc gaccctagac ctccacctag aagaatcgcc gtgcccagca gatccagcgc
      361 cagcgtgcca aaacctgccc cccagcctta ccccttcacc agcagcctga gcaccatcaa
      421 ctacgacgag ttccctacca tggtgttccc cagcggccag atctctcagg cctctgctct
      481 ggctccagcc cctcctcagg tgctgcctca ggctcctgct cctgcaccag ctccagccat
      541 ggtgtctgca ctggctcagg caccagcacc cgtgcctgtg ctggctcctg gacctccaca
      601 ggctgtggct ccaccagccc ctaaacctac acaggccggc gagggcacac tgtctgaagc
      661 tctgctgcag ctgcagttcg acgacgagga tctgggagcc ctgctgggaa acagcaccga
      721 tcctgccgtg ttcaccgacc tggccagcgt ggacaacagc gagttccagc agctgctgaa
      781 ccagggcatc cctgtggccc ctcacaccac cgagcccatg ctgatggaat accccgaggc
      841 catcacccgg ctcgtgacag gcgctcagag gcctcctgat ccagctcctg cccctctggg
      901 agcaccaggc ctgcctaatg gactgctgtc tggcgacgag gacttcagct ctatcgccga
      961 tatggatttc tcagccttgc tgggctctgg cagcggcagc cgggattcca gggaagggat
     1021 gtttttgccg aagcctgagg ccggctccgc tattagtgac gtgtttgagg gccgcgaggt
     1081 gtgccagcca aaacgaatcc ggccatttca tcctccagga agtccatggg ccaaccgccc
     1141 actccccgcc agcctcgcac caacaccaac cggtccagta catgagccag tcgggtcact
     1201 gaccccggca ccagtccctc agccactgga tccagcgccc gcagtgactc ccgaggccag
     1261 tcacctgttg gaggatcccg atgaagagac gagccaggct gtcaaagccc ttcgggagat
     1321 ggccgatact gtgattcccc agaaggaaga ggctgcaatc tgtggccaaa tggacctttc
     1381 ccatccgccc ccaaggggcc atctggatga gctgacaacc acacttgagt ccatgaccga
     1441 ggatctgaac ctggactcac ccctgacccc ggaattgaac gagattctgg ataccttcct
     1501 gaacgacgag tgcctcttgc atgccatgca tatcagcaca ggactgtcca tcttcgacac
     1561 atctctgtt
//
