LOCUS       sequence-457503-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  p65 activation domain coupled to reverse Tet repressor; labeled with
            GFP; binds tetO sites upon doxycycline addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     HP1 binding creates a local barrier against transcription activation
            and persists during chromatin decondensation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: rTetR-GFP-p65
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             425..778
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSAL"
ORIGIN
        1 ccagtacctg cccgacaccg acgaccggca ccggatcgag gaaaagcgga agcggaccta
       61 cgagacattc aagagcatca tgaagaagtc ccccttcagc ggccccaccg accctagacc
      121 tccacctaga agaatcgccg tgcccagcag atccagcgcc agcgtgccaa aacctgcccc
      181 ccagccttac cccttcacca gcagcctgag caccatcaac tacgacgagt tccctaccat
      241 ggtgttcccc agcggccaga tctctcaggc ctctgctctg gctccagccc ctcctcaggt
      301 gctgcctcag gctcctgctc ctgcaccagc tccagccatg gtgtctgcac tggctcaggc
      361 accagcaccc gtgcctgtgc tggctcctgg acctccacag gctgtggctc caccagcccc
      421 taaacctaca caggccggcg agggcacact gtctgaagct ctgctgcagc tgcagttcga
      481 cgacgaggat ctgggagccc tgctgggaaa cagcaccgat cctgccgtgt tcaccgacct
      541 ggccagcgtg gacaacagcg agttccagca gctgctgaac cagggcatcc ctgtggcccc
      601 tcacaccacc gagcccatgc tgatggaata ccccgaggcc atcacccggc tcgtgacagg
      661 cgctcagagg cctcctgatc cagctcctgc ccctctggga gcaccaggcc tgcctaatgg
      721 actgctgtct ggcgacgagg acttcagctc tatcgccgat atggatttct cagccttgct
//
