LOCUS       sequence-457528-        1080 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lac repressor (LacI) which binds lacO sites; labeled with HaloTag 
            and coupled with Kruppel-associated box (KRAB) repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1080)
  TITLE     HP1 binding creates a local barrier against transcription activation
            and persists during chromatin decondensation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-LacI-KRAB
FEATURES             Location/Qualifiers
     source          1..1080
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..46)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cgaaatggtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca
       61 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt
      121 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg
      181 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca
      241 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat
      301 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg
      361 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc
      421 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag
      481 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg
      541 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct
      601 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg
      661 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt
      721 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac
      781 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga
      841 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca
      901 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
      961 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc
     1021 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gaagcagcct
//
