LOCUS       sequence-457529-         309 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lac repressor (LacI) which binds lacO sites; labeled with HaloTag 
            and coupled with Kruppel-associated box (KRAB) repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 309)
  TITLE     HP1 binding creates a local barrier against transcription activation
            and persists during chromatin decondensation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: HaloTag-LacI-KRAB
FEATURES             Location/Qualifiers
     source          1..309
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..222
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQILYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
     CDS             289..309
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gatgctaagt cactgactgc ctggtcccgg acactggtga ccttcaagga tgtgtttgtg
       61 gacttcacca gggaggagtg gaagctgctg gacactgctc agcagatcct gtacagaaat
      121 gtgatgctgg agaactataa gaacctggtt tccttgggtt atcagcttac taagccagat
      181 gtgatcctcc ggttggagaa gggagaagag ccctggctgg tggagagaga aattcaccaa
      241 gagacccatc ctgattcaga gactgcattt gaaatcaaat catcagttcc gaaaaagaaa
      301 cgcaaagtt
//
