LOCUS       sequence-457672-        8785 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pgpc-1 wCherry unc-54 3' UTR C.elegans neuron and other structures 
            expression of RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8785)
  AUTHORS   Lin A, Qin S, Casademunt H, Wu M, Hung W, Cain G, Tan NZ, Valenzuela
            R, Lesanpezeshki L, Venkatachalam V, Pehlevan C, Zhen M, Samuel ADT
  TITLE     Functional imaging and quantification of multineuronal olfactory 
            responses in C. elegans.
  JOURNAL   Sci Adv. 2023 Mar;9(9):eade1249. doi: 10.1126/sciadv.ade1249. Epub 
            2023 Mar 1.
  PUBMED    36857454
REFERENCE   2  (bases 1 to 8785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03"; volume: "9(9):eade1249. doi"; pages: " 
            10.1126/sciadv.ade1249. Epub 2023 Mar 1"
COMMENT     Sequence Label: pJH4040
FEATURES             Location/Qualifiers
     source          1..8785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(820..831)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     primer_bind     complement(6226..6248)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6348..6367
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6445..6463)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6531..6635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6636..6704,6705..7496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6854..6873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7667..8255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8156..8175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8409..8426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8543..8564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8579..8584,8585..8602,8603..8609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8617..8633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8622..8644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8641..8657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8641..8657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taagcttggg ctgcagcatg cggccgctaa gcacattcga ttctctagag attgtggaat
       61 gacgaaatta atgaataacg ttttcgtctt tccattagag ttttatattt ttaatcaaaa
      121 gttacaacta aacttcatac tagtaatctt catattcaag agtcaatcta gttccagtca
      181 aatattaatg tatagagcca atgtaagttt gaagattatc aagcgtatga aggtatcatg
      241 caacgagcta atataagacg tttttccaaa gtagaatgac cttttctaca cttgctgagg
      301 gaattggaac gttgtcactt atgtatgtct gataatttag tgcatagtct gaaatgaaaa
      361 agttctgcaa gttacatatt catgtgattt catgcaattg agatgcttaa ttttagttgt
      421 tcttcatatt atcaactttc aaaatttcta aaaaatggct tcattgaagt ttaggtacaa
      481 atttaataaa ctataaaagc aattgtttga gaaatgtttc gtagaaatag gaaaattgtc
      541 ggatttttct gaaaatttaa taatctggga caccccattt taggtccgta aagtatatgt
      601 cgtcctcaag attgcaattt gtatgaaatg ttattaacgt attcaaattg tatgttctat
      661 gttgttttaa ttggagtagt tttgttggat gacaaagcta tgttgaataa tttcgaattt
      721 attttgtggt ttaaactagt gtatattatt atttatacct ataagcacat gacacatata
      781 tagatatcca ttcacactaa ctcataagag agtacgttgc actttgtgat tagtgatttc
      841 acaaccctct tctgaatata cttactacat tgaaatctaa atgattaaat ttctttgaac
      901 taatggtttc tttatccgat attagttata atctttgttg aaaaacgata cttaaaattt
      961 tttttactta agtttaacca cattttacaa aacgatattc aagcaaatac atatttttaa
     1021 gcttgcgtgg ggaaaattat tacattagtc tgaaacgatt atgtcgtgag acagaacaaa
     1081 gttctgggaa aaattcagct ctgctacttc cataaaaatt ggttattgac aaaaagtgat
     1141 cgtcgaatta atttttacat ctagcatact atttcggttt tgtttaaggt ttcctcatga
     1201 atttgcacaa ggcttgcatt gcaataagtt tcaattgaga ttgaaatgaa aataatcaca
     1261 tattttccca atttgactaa atttatcact catatatgga ttaaaatttt ttcccattta
     1321 tgcttttcaa aaaggaagaa gatttggttt caaacccatc tgatattttt tgtcaataat
     1381 ctaaaaaaaa ctccaagtat gtaatcattt tcttctccac taagccgaaa ataagatttg
     1441 atggttgaat atttactcga atcttgtgac ggatataaaa caaagtatac tacaaaaact
     1501 agatgttact agtacattct tctaattcac aacttcattt tcaagtggaa atttgctaaa
     1561 cacctgtttc ctagtcaaat tgggttaatg ttttgtgctt ttctgagtat tttctcatat
     1621 ggaaaatgtt gtttctatac aacatcaagt tagatacata ttgtagctag catttcgatt
     1681 cttgaaaagt tcaacttcac tttgtatgtg cgcaaacgtg actctttttt acttttattg
     1741 taaagtgcgt tttcattttc cgttaaattg aacaccctgt taaattactc ttgtttacct
     1801 ttttgtaggc attcttgcta cctacctgct aattctctgc acaaacgcgt cgaagttcag
     1861 aactataacg tcatctcaga gaagttgaag aaaaataaca atcaagtaat aactacgtaa
     1921 tcactattag tatctcttta ttttatatag tttccgcaga tgacgaagtt gtgaaacaaa
     1981 taaacaatac atgttttgtt tgaaaatggg taaatggggt aggtgtgtgt agtttttttt
     2041 taaggatgtc atgtgggcgg gactattatg tcatcatatt agttctttct tgagatttca
     2101 aatttatgac tcttatgcaa tcggcattta agtttacttt gatttaaaaa aattaaaaca
     2161 aatgggctta cgaagtcccc gaatgttagt ctcatccaac ttggcgctat attaaattat
     2221 gataagaagc tgcaaaaaca tgttcaaatg tattttattg acaacaaaag gtggcaatta
     2281 cttgattatt gccttttttg agaatactat agcactggag tatgtccgaa atcgtacagt
     2341 gtttgaacac taatatttga acttatagtt aagttaacca aaaaattttt aagaaaaatg
     2401 tttttgatca atttccaaaa aatggtagtt ttaattagtc tcatttttag aaacaataaa
     2461 aaaattttta aaaatttgtt aaaaatttta ctataatatt tagccatttt ggcaccatga
     2521 gagtggtttt taaattttta ccttgttctg ctgcttcacc tttaatgaca gttcgatata
     2581 taattatact acttaatttt cattagttta attttaatat tgccagtttg tttgactata
     2641 tatttctatt agttaccaca aaacctaatc acctttcttt gcagctttgt caaaatatga
     2701 ttagagtata aaacgcatag ctcatttagt aactatgggc aaccacacct aataaaagtt
     2761 tggataagaa agctgattgg tacgagttat gctatctggt tatgaaatga tgaaaagaaa
     2821 acataaaaca ggtgaaagta aagagcgagc tgctgacttg ttgagttttc tggactcttt
     2881 tttttttgta aagattttgt taaaatcacg aaaactcgac agtaactcta gtatagctcg
     2941 tttctttgga ttttacttct gactcacgaa gcgttaaatt ttgttttgaa tagtgaagag
     3001 atttatgtgg tttatgctta acgttttaat tatgggagtt atcagcaatg aaagcttttt
     3061 acatatatac aataagatcc atttaaagcg tataaaaagt ttagttctgt ccaaataaaa
     3121 acgaaaattg gggtttcaca ttatttgaga acctagttgt ataattttca gtaattttca
     3181 tattcttctc agtttgttat aattttctgc tgtccaacgc aattttcgtt ttattaaaat
     3241 agcatataaa ataatttaat tttgattaaa attcaagaaa tactaaatgt gatcgtaagc
     3301 tattaactaa atacaatttc gtgttgaata ttttcaaagt cagaaagtta atacaaatca
     3361 tcttgcctgt tcctgatttt ttacaaataa caaatctagg aatcgagaca aaaaaccaaa
     3421 tctatattca aatggtcact gacaaatttt tgaatacaaa tccaatcaaa acaactataa
     3481 agagttggat tcgattcatt cagttcttgt tttgatcaac agagctttgc aaactgagtg
     3541 atttttagat tgagaaagaa atgagttagt agaagtatta gtttttaaga attttagccg
     3601 caacgtattt tgtatggtta ttttgatgaa ttattaaaaa ttaacatttt tctggccaat
     3661 ttgatattta cgtataaatt atattgaatg tagcatatca aaaatattca aaaatgttca
     3721 ataataaaat caatgttaaa tccgctggcg ccaaaatccc tccgaatccc ctgaattgtg
     3781 gattgactaa gagtaaaagg aagagaaacg aaaaagggag ggcgacttaa tcccgaacaa
     3841 tattttttat gatgaagagc gcatagggcc gtcgtcagtt ctcacactgg atttcacttt
     3901 ttaccgcctc tcttttgtcg tgaagagtct cagaatccag atgtaaaatc ctctccaatc
     3961 tatttattaa acatagctca gtcttcaatc ttttaattat aaacaccgtt ttagcacatg
     4021 agctcttctg cctcgaaacc ctttaatttc gacaaaagag caaatgctcc gatcgtaaaa
     4081 ttggtagttt cacccaccaa acctactcat tttgcgtcga caccaaactt gtctcctcgc
     4141 tttttttctt cccagctacc ttactcattt caccagtttt cgctattgag aagaagattt
     4201 gttttatcat attttgaaaa tttattagcg tttctttcag ttccagaaac catctgagcc
     4261 cgggattggc caaaggaccc aaaggtatgt ttcgaatgat actaacataa catagaacat
     4321 tttcaggagg acccttggct agcgtcgacg gtaccctatg gtctcaaagg gtgaagaaga
     4381 taacatggca attattaaag agtttatgcg tttcaaggtg catatggagg gatctgtcaa
     4441 tgggcatgag tttgaaattg aaggtgaagg agaaggccga ccatatgagg gaacacaaac
     4501 cgcaaaacta aaggtaagtt taaacatata tatactaact aaccctgatt atttaaattt
     4561 tcaggtaact aaaggcggac cattaccatt cgcctgggac atcctctctc cacagttcat
     4621 gtatggaagt aaagcttatg ttaaacatcc ggcagatata ccagattatt tgaaactttc
     4681 attcccggag ggttttaagt gggaacgcgt aatgaatttt gaagacggag gagttgttac
     4741 agtgacgcaa gactcaaggt aagtttaaac agttcggtac taactaacca tacatattta
     4801 aattttcagc ctccaagatg gagaatttat ttataaagtc aaacttcgag gaacgaattt
     4861 cccctcggat ggatctgtta tgcagaagaa gactatggga tgggaagctt caagtgaaag
     4921 aatgtaccct gaagacggtg ctcttaaggg agagattaaa caacgtctta aattgaaaga
     4981 tggaggacat tacgatgctg aggtaagttt aaacatgatt ttactaacta actaatctga
     5041 tttaaatttt caggtgaaga caacttacaa agccaaaaaa ccagttcagc tgccaggagc
     5101 gtacaatgtt aatattaaac tggatatcac ctcccacaac gaggattaca ctatcgttga
     5161 gcaatatgaa agagctgaag ggcggcactc gacaggtggc atggatgaat tgtataagta
     5221 ggagctcccg agatcttcag cgctcagcat tcgtagaatt gtaagtttaa actttctcat
     5281 actaactaac gcgctctatt taaattttca gaattccaac tgagcgccgg tcgctaccat
     5341 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     5401 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     5461 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     5521 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     5581 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     5641 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     5701 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     5761 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     5821 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     5881 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     5941 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     6001 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     6061 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     6121 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     6181 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     6241 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     6301 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     6361 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     6421 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     6481 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8521 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8581 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8641 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
     8701 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     8761 atacgctaac aacttggaaa tgaaa
//
