LOCUS       sequence-457694-       12319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding STING-mNeonGreen under UbC 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12319)
  AUTHORS   Gentili M, Liu B, Papanastasiou M, Dele-Oni D, Schwartz MA, Carlson 
            RJ, Al'Khafaji AM, Krug K, Brown A, Doench JG, Carr SA, Hacohen N
  TITLE     ESCRT-dependent STING degradation inhibits steady-state and 
            cGAMP-induced signalling.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):611. doi: 10.1038/s41467-023-36132-9.
  PUBMED    36739287
REFERENCE   2  (bases 1 to 12319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):611. doi"; pages: " 
            10.1038/s41467-023-36132-9"
COMMENT     Sequence Label: pTRIP-UbC-Blast-2A-STING-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..12319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3944..3963
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      4121..4130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4127..4522
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4538..4594
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             6723..6903
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8431..8450)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8664..8683)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8783..8805
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8843..8861)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8929..9033
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9034..9102,9103..9894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9252..9271)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10065..10653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10554..10573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10807..10824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10894..10914)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10899..11228
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11079..11214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtggcctccg cgccgggtgt ggcacagcta gttccgtcgc
     3241 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     3301 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     3361 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     3421 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     3481 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     3541 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     3601 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     3661 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     3721 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     3781 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     3841 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     3901 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     3961 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     4021 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     4081 tggccgtttt tggctttttt tgttagaccc gcgggctagc gccaccatgg ccaagccttt
     4141 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     4201 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     4261 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     4321 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     4381 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     4441 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     4501 ctctggttat gtgtgggagg gccgtacggg aagcggagct actaacttca gcctgctgaa
     4561 gcaggctgga gacgtggagg agaaccctgg acctggatcc atgccccact ccagcctgca
     4621 tccatccatc ccgtgtccca ggggtcacgg ggcccagaag gcagccttgg ttctgctgag
     4681 tgcctgcctg gtgacccttt gggggctagg agagccacca gagcacactc tccggtacct
     4741 ggtgctccac ctagcctccc tgcagctggg actgctgtta aacggggtct gcagcctggc
     4801 tgaggagctg cgccacatcc actccaggta ccggggcagc tactggagga ctgtgcgggc
     4861 ctgcctgggc tgccccctcc gccgtggggc cctgttgctg ctgtccatct atttctacta
     4921 ctccctccca aatgcggtcg gcccgccctt cacttggatg cttgccctcc tgggcctctc
     4981 gcaggcactg aacatcctcc tgggcctcaa gggcctggcc ccagctgaga tctctgcagt
     5041 gtgtgaaaaa gggaatttca acgtggccca tgggctggca tggtcatatt acatcggata
     5101 tctgcggctg atcctgccag agctccaggc ccggattcga acttacaatc agcattacaa
     5161 caacctgcta cggggtgcag tgagccagcg gctgtatatt ctcctcccat tggactgtgg
     5221 ggtgcctgat aacctgagta tggctgaccc caacattcgc ttcctggata aactgcccca
     5281 gcagaccggt gaccgtgctg gcatcaagga tcgggtttac agcaacagca tctatgagct
     5341 tctggagaac gggcagcggg cgggcacctg tgtcctggag tacgccaccc ccttgcagac
     5401 tttgtttgcc atgtcacaat acagtcaagc tggctttagc cgggaggata ggcttgagca
     5461 ggccaaactc ttctgccgga cacttgagga catcctggca gatgcccctg agtctcagaa
     5521 caactgccgc ctcattgcct accaggaacc tgcagatgac agcagcttct cgctgtccca
     5581 ggaggttctc cggcacctgc ggcaggagga aaaggaagag gttactgtgg gcagcttgaa
     5641 gacctcagcg gtgcccagta cctccacgat gtcccaagag cctgagctcc tcatcagtgg
     5701 aatggaaaag cccctccctc tccgcacgga tttctctact agtatggtca gcaaaggcga
     5761 agaagacaac atggcatcac tgccagcgac acacgaactt catattttcg gctccatcaa
     5821 tggtgtggac ttcgatatgg ttggacaggg tactgggaac cctaacgatg gatatgagga
     5881 gttgaacttg aagagtacca agggcgatct tcagttttca ccttggattc tcgtacctca
     5941 tataggttat ggctttcatc aatatctgcc ctatcctgac ggtatgtcac ctttccaagc
     6001 agctatggtt gacgggagcg gttatcaggt tcaccggaca atgcagtttg aagatggagc
     6061 ctctttgacc gtaaattatc ggtatactta tgagggcagc catattaaag gcgaggctca
     6121 agttaagggg actgggttcc ccgcggatgg tcccgtaatg acaaacagtc tgacggctgc
     6181 agactggtgc cgctctaaga agacatatcc aaatgacaag acgattatta gcactttcaa
     6241 gtggtcatac actacaggaa atggtaaacg ctataggagc actgcccgga ccacgtatac
     6301 tttcgcgaag ccgatggcgg caaattattt gaaaaaccaa ccgatgtatg tgttcagaaa
     6361 gacagaactc aagcattcta aaactgaact gaacttcaaa gagtggcaga aagcctttac
     6421 tgatgtaatg ggcatggacg aactctataa gtaactcgag acctagaaaa acatggagca
     6481 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     6541 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     6601 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     6661 tcccaacgaa gacaaaatcg tcgagagatg ctgcatataa gcagctgctt tttgcttgta
     6721 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6781 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6841 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6901 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6961 cagagagtga gaggccttga cattataata gatttagcag gaattgaact aggagtggag
     7021 cacacaggca aagctgcaga agtacttgga agaagccacc agagatactc acgattctgc
     7081 acatacctgg ctaatcccag atcctaagga ttacattaag tttactaaca tttatataat
     7141 gatttatagt ttaaagtata aacttatcta atttactatt ctgacagata ttaattaatc
     7201 ctcaaatatc ataagagatg attactatta tccccattta acacaagagg aaactgagag
     7261 ggaaagatgt tgaagtaatt ttcccacaat tacagcatcc gttagttacg actctatgat
     7321 cttctgacac aaattccatt tactcctcac cctatgactc agtcgaatat atcaaagtta
     7381 tggacattat gctaagtaac aaattaccct tttatatagt aaatactgag tagattgaga
     7441 gaagaaattg tttgcaaacc tgaatagctt caagaagaag agaagtgagg ataagaataa
     7501 cagttgtcat ttaacaagtt ttaacaagta acttggttag aaagggattc aaatgcataa
     7561 agcaagggat aaatttttct ggcaacaaga ctatacaata taaccttaaa tatgacttca
     7621 aataattgtt ggaacttgat aaaactaatt aaatattatt gaagattatc aatattataa
     7681 atgtaattta cttttaaaaa gggaacatag aaatgtgtat cattagagta gaaaacaatc
     7741 cttattatca caatttgtca aaacaagttt gttattaaca caagtagaat actgcattca
     7801 attaagttga ctgcagattt tgtgttttgt taaaattaga aagagataac aacaatttga
     7861 attattgaaa gtaacatgta aatagttcta catacgttct tttgacatct tgttcaatca
     7921 ttgatcgaag ttctttatct tggaagaatt tgttccaaag actctgaaat aaggaaaaca
     7981 atctattata tagtctcaca cctttgtttt acttttagtg atttcaattt aataatgtaa
     8041 atggttaaaa tttattcttc tctgagatca tttcacattg cagatagaaa acctgagact
     8101 ggggtaattt ttattaaaat ctaatttaat ctcagaaaca catctttatt ctaacatcaa
     8161 tttttccagt ttgatattat catataaagt cagccttcct catctgcagg ttccacaaca
     8221 aaaatccaac caactgtgga tcaaaaatat tgggaaaaaa ttaaaaatag caatacaaca
     8281 ataaaaaaat acaaatcaga aaaacagcac agtataacaa ctttatttag catttacaat
     8341 ctattaggta ttataagtaa tctagccaga tcctctacgc cggacgcatc gtggccggca
     8401 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     8461 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     8521 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     8581 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     8641 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8701 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8761 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8821 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     8881 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     8941 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     9001 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     9061 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     9121 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     9181 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     9241 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     9301 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     9361 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     9421 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     9481 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     9541 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     9601 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     9661 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     9721 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     9781 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     9841 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     9901 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     9961 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
    10021 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
    10081 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
    10141 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
    10201 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
    10261 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
    10321 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
    10381 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
    10441 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
    10501 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
    10561 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
    10621 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
    10681 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
    10741 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
    10801 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
    10861 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
    10921 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10981 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    11041 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    11101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    11161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    11221 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
    11281 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
    11341 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
    11401 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
    11461 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
    11521 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
    11581 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
    11641 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
    11701 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
    11761 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
    11821 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
    11881 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
    11941 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    12001 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    12061 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
    12121 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
    12181 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
    12241 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
    12301 accttacttc tgtggtgtg
//
