LOCUS       sequence-457696-       10800 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for DKK1 and BFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10800)
  AUTHORS   Yamada T, Trentesaux C, Brunger JM, Xiao Y, Stevens AJ, Martyn I, 
            Kasparek P, Shroff NP, Aguilar A, Bruneau BG, Boffelli D, Klein OD, 
            Lim WA
  TITLE     Synthetic organizer cells guide development via spatial and 
            biochemical instructions.
  JOURNAL   Cell. 2024 Dec 11:S0092-8674(24)01323-0. doi: 
            10.1016/j.cell.2024.11.017.
  PUBMED    39706189
REFERENCE   2  (bases 1 to 10800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 11:S0092-8674(24)01323-0. doi: 10.1016/j.cell.2024.11.017."
COMMENT     Sequence Label: aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE
FEATURES             Location/Qualifiers
     source          1..10800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1086..1659
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1253..1270)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1480..1499
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1689..2387
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    2402..2990
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2455..2475)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2873..2884)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3012..3028)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3013..3029)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3119..3352
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3377..3395)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3378..3395)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3685..3704)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3918..3937)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4037..4059
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4097..4115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      complement(4294..4882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4374..4393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(5096..5115)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5107..5922
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5107..5922
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5180..5199)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6097..6114
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6184..6204)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6189..6518
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6369..6504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7652..7717
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7847..7867
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8292..8426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8329..8348)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8383..8402
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8594..9227
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9274..9399
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9896..10129
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10314..10358
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10643..10760
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgatgagac gaacgatcga taagcttgat atcgaattcg gagcactgtc ctccgaacgt
       61 cggagcactg tcctccgaac gtcggagcac tgtcctccga acgtcggagc actgtcctcc
      121 gaacggagca tgtcctccga acgtcggagc actgtcctcc gaacgactag tctagagggt
      181 atataatggg ggccactagt ctactaccag agctcatcgc tagcgctacc ggatcgccac
      241 ctatgatggt tgtgtgtgca gcggcagctg tccggttctt ggccgtgttt acaatgatgg
      301 ctctctgcag cctccctctg ctaggagcca gtgccacctt gaactcagtt ctcatcaatt
      361 ccaacgcgat caagaacctg cccccaccgc tgggtggtgc tggggggcag ccgggctctg
      421 ctgtcagtgt ggcgccggga gttctctatg agggcgggaa caagtaccag actcttgaca
      481 actaccagcc ctacccttgc gctgaagatg aggagtgcgg ctctgacgag tactgctcca
      541 gccccagccg cggggcagcc ggcgtcggag gtgtacagat ctgtctggct tgccgaaagc
      601 gcaggaagcg ctgcatgagg cacgctatgt gctgccccgg gaactactgc aaaaatggaa
      661 tatgcatgcc ctctgaccac agccattttc ctcgagggga aattgaggaa agcatcattg
      721 aaaaccttgg taatgaccac aacgccgccg cgggggatgg atatcccaga agaaccacac
      781 tgacttcaaa aatatatcac accaaaggac aagaaggctc cgtctgcctc cgatcatcag
      841 actgtgccgc agggctgtgt tgtgcaagac acttctggtc caagatctgt aaacctgtcc
      901 ttaaagaagg tcaggtgtgc accaagcaca aacggaaagg ctcccacggg ctggagatat
      961 tccagcgctg ttactgcggg gaaggcctgg cttgcaggat acagaaagat caccatcaag
     1021 ccagcaattc ttctaggctc cacacctgcc agagacacgg ttcttaagat ccttgacttg
     1081 cggcccccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     1141 cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg
     1201 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     1261 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     1321 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     1381 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     1441 tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     1501 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     1561 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     1621 cacggggacg tggttttcct ttgaaaaaca cgatgataaa tgagcgctgg cggaccacca
     1681 tgggatccag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     1741 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     1801 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     1861 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     1921 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     1981 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     2041 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     2101 gctgggaggc cttcaccgaa acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     2161 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     2221 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     2281 tggaaagaat caaggaggcc aacaacgaaa cctacgtcga gcagcacgag gtggcagtgg
     2341 ccagatactg cgacctccct agcaaactgg ggcacaagct taattaatgg cgcttatcga
     2401 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2461 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2521 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2581 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2641 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2701 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2761 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     2821 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     2881 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     2941 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atgctgcgtc
     3001 tcaagcatac acgataccgt cgacctcgag ggaattaatt cgagctcggt acctttaaga
     3061 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     3121 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtttctc
     3181 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     3241 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     3301 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagcatctag
     3361 aattaattcc gtgtattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
     3421 tgtattaatt gtagccgcgt tctaacgaca atatgtacaa gcctaattgt gtagcatctg
     3481 gcttactgaa gcagacccta tcatctctct cgtaaactgc cgtcagagtc ggtttggttg
     3541 gacgaacctt ctgagtttct ggtaacgccg tcccgcaccc ggaaatggtc agcgaaccaa
     3601 tcagcagggt catcgctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg
     3661 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg
     3721 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg
     3781 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca
     3841 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc
     3901 gaatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     3961 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     4021 caagctgtga ccatctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     4081 cgcgcgagat gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     4141 atggtttctt agacgtcagg tggcactttt cggggaaatg tgccgagcgg ccgcacggtt
     4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgact gatccttcaa ctcagcaaaa
     4981 gttcgattta ttcaacaaag ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa
     5041 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     5101 ggtgttatga gccatattca acgggaaacg tcttgctcca ggccgcgatt aaattccaac
     5161 atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg
     5221 acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa
     5281 ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt
     5341 atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc
     5401 actgcgatcc ccgggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa
     5461 aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat
     5521 tgtcctttta acagcgatcg cgtatttcgc ctcgctcagg cgcaatcacg aatgaataac
     5581 ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc
     5641 tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac tcatggtgat
     5701 ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga
     5761 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
     5821 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
     5881 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
     5941 ttgtaacacg cggccgccaa ttttttacgg ttcctggcct tttgctggcc ttttgctcac
     6001 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6061 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6121 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6181 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     6241 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     6301 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     6361 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6421 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6481 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     6541 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     6601 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     6661 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     6721 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     6781 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     6841 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     6901 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     6961 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     7021 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     7081 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     7141 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     7201 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     7261 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     7321 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     7381 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     7441 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     7501 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     7561 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     7621 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     7681 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     7741 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     7801 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     7861 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     7921 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     7981 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     8041 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     8101 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     8161 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     8221 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     8281 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8401 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     8461 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     8521 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     8581 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     8641 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     8701 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     8761 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     8821 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     8881 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     8941 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     9001 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactggga ctctctggtt
     9061 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     9121 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     9181 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     9241 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     9301 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     9361 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     9421 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     9481 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     9541 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     9601 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     9661 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     9721 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     9781 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     9841 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     9901 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     9961 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
    10021 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
    10081 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
    10141 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
    10201 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
    10261 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
    10321 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
    10381 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
    10441 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
    10501 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
    10561 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
    10621 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
    10681 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
    10741 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggccct
//
