LOCUS       sequence-457735-        2760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Scherschel M, Niemeier JO, Jacobs LJHC, Hoffmann MDA, Diederich A, 
            Bell C, Hohne P, Raetz S, Kroll JB, Steinbeck J, Lichtenauer S, 
            Multhoff J, Zimmermann J, Sadhanasatish T, Rothemann RA, Grashoff C,
            Messens J, Ampofo E, Laschke MW, Riemer J, Roma LP, Schwarzlander M,
            Morgan B
  TITLE     A family of NADPH/NADP(+) biosensors reveals in vivo dynamics of 
            central redox metabolism across eukaryotes.
  JOURNAL   Nat Commun. 2024 Dec 19;15(1):10704. doi: 
            10.1038/s41467-024-55302-x.
  PUBMED    39702652
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12-19"; volume: "15(1):10704. doi"; pages: " 
            10.1038/s41467-024-55302-x"
COMMENT     Sequence Label: pcDNA3.1(+) NAPstar3b
FEATURES             Location/Qualifiers
     source          1..2760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(2195..2213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgaaggtgc cagaggccgc catcagcagg ctgatcacct acctgcgcat cctggaggag
       61 ctggaggcac agggagtgca cagaacagcc tccgagcagc tgggagagct ggcccaggtg
      121 accgccttcc aggtggacaa ggatctgagc tactttggct cctatggcac cgacggcgtg
      181 ggctatacag tgcccgtgct gaagcgggag ctgagacaca tcctgggcct gaacaggaag
      241 tggggactgt gcatcgtggg aatgggccgc ctgggctctg ccctggccga ttggccagga
      301 ttcggagaga gctttgagct gaggggcttc ttttcccgca aagccgaaaa agtgggcagg
      361 cccgtgcgcg gaggctatat cgagcacgtg gacctgctgc cacagcgggt gcccggcaga
      421 atcgagatcg ccctgctgac agtgcctagg gaggcagcac agaaggcagc agatctgctg
      481 gtggccgccg gcatcaaggg catcctgaac ttcgccccag tggtgctgga ggtgcccaag
      541 gaggtggccg tggagaatgt ggacatcctg gccggcctga ccagactgag cttcgccatc
      601 ctgaacccaa catggtccgc cgcaggagga cacggattta ccgcacacaa cgtgtacatc
      661 atggccgata agcagaagaa cggcatcaag gccaacttca agatcaggca caacatcgag
      721 gacggaggag tgcagctggc cgatcactac cagcagaaca ccccaatcgg agacggacct
      781 gtgctgctgc cagataatca ctatctgtcc atccagtcta agctgagcaa ggaccctaat
      841 gagaagcgcg atcacatggt gctgctggag ttcgtgaccg cagcaggaat cacacacgga
      901 atggacgagc tgtacaaggg cggcacaggc ggctccatgg tgtctaaggg agaggagctg
      961 ttcaccggag tggtgcccat cctggtggag ctggacggcg atgtgaacgg ccacaagttt
     1021 agcgtgtccg gagagggaga gggagatgca acctatggca agctgacact gaagttcatc
     1081 tgcaccacag gcaagctgcc cgtgccttgg ccaacactgg tgaccacatt ctcctacggc
     1141 gtgatggtgt ttgcccgcta tcccgaccac atgaagcagc acgatttctt taagtctgcc
     1201 atgcctgagg gctacgtgca ggagcggacc atcttcttta aggacgatgg caactataag
     1261 acaagagccg aggtgaagtt cgagggcgac accctggtga ataggatcga gctgaagggc
     1321 atcgacttta aggaggatgg caacatcctg ggccacaagc tggagtacaa tacaaaggtg
     1381 cctgaggccg ccatctctcg gctgatcacc tatctgagaa tcctggagga actggaggcc
     1441 cagggcgtgc accggaccgc ctccgaacag ctgggcgagc tggcccaggt cactgccttc
     1501 caggtggacg aggatctgtc ttactttggc agctacggca ccgatggcgt gggctacaca
     1561 gtgccagtgc tgaaaagaga actgaggcac atcctgggcc tgaatcggaa gtggggcctg
     1621 tgcattgtcg gaatgggcag actgggctcc gccctggccg attggcctgg attcggcgag
     1681 tcttttgagc tgagaggctt cttcagccgg aaagccgaaa aagtcggcag gccagtgcgc
     1741 ggaggagtca ttgaacatta tgatctgctg ccacagaggg tgcctggaag gattgaaatc
     1801 gccctgctga ccgtgcccag ggaggcagcc cagaaggccg ccgacctgct ggtggctgct
     1861 ggaattaaag gcatcctgaa ttttgcccct gtggtgctgg aggtgccaaa agaagtcgct
     1921 gtggagaatg tggacttcct ggccggcctg acaaggctga gctttgccat cctgaaccct
     1981 aagtggaggg aggagatgat gggctccggc accggaggaa atgcctccga cggaggcggc
     2041 tctggcggca tggtcagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     2101 agatttaagg tgcacatgga gggcagcgtg aatggccacg agtttgagat cgaaggagag
     2161 ggagagggca ggccttacga gggcacccag acagccaagc tgaaggtgac caagggagga
     2221 ccactgcctt tcgcctggga catcctgtcc ccacagttta tgtacggctc taaggcctat
     2281 gtgaagcacc cagccgacat ccccgattat ctgaagctga gcttccccga gggctttaag
     2341 tgggagcgcg tgatgaactt cgaggatggc ggcgtggtga ccgtgacaca ggacagctcc
     2401 ctgcaggatg gcgagttcat ctacaaggtg aagctgcggg gcacaaattt tccttccgac
     2461 ggcccagtga tgcagaagaa gaccatgggc tgggaggcct ctagcgagcg catgtatccc
     2521 gaggatggcg ccctgaaggg cgagatcaag cagcggctga agctgaagga cggcggccac
     2581 tacgatgccg aggtgaagac cacatataag gccaagaagc cagtgcagct gcctggagcc
     2641 tacaacgtga atatcaagct ggacatcacc tctcacaacg aggattacac aatcgtggag
     2701 cagtatgaga gggcagaggg cagacacagc accggaggca tggacgagct gtataagtga
//
