LOCUS       sequence-457743-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral or overpexression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Klingbeil O, Skopelitis D, Tonelli C, Yoshimoto T, Alpsoy A, 
            Panepinto MC, Minicozzi F, Merrill JR, Cafiero AM, Aggarwal D, Russo
            S, Ha T, Demerdash OE, Wee TL, Spector DL, Lyons SK, Tuveson DA, 
            Cifani P, Vakoc CR
  TITLE     MARK2/MARK3 kinases are catalytic co-dependencies of YAP/TAZ in 
            human cancer.
  JOURNAL   Cancer Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529.
  PUBMED    39058094
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2024 Jul 26. doi: 10.1158/2159-8290.CD-23-1529."
COMMENT     Sequence Label: FLAG-MARK2-CR
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2149..2172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(4357..4931,4932..4934,4935..4943)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4524..4541)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4751..4770
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5382..5970
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5435..5455)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5853..5864)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6042..6275
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6353..6474
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6390..6409)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6444..6463
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6514..6649
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6669..6688)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6670..6688)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6698..6715)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6698..6714)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6707..6729)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6856..7311
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6943..6962)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7153..7174
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7337..7441
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7442..7510,7511..8302)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7660..7679)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8473..9061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8962..8981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9215..9232
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9349..9370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9385..9390,9391..9408,9409..9415)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9423..9439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9428..9450
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9447..9463
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9447..9463
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9482..9502
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9484..9502
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac
     2161 gatgacgata agatgtccag cgctcggacc cccctaccca cgctgaacga gagggacacg
     2221 gagcagccca ccttgggaca ccttgactcc aagcccagca gtaagtccaa catgattcgg
     2281 ggccgcaact cagccacctc tgctgatgag cagccccaca ttggaaacta ccggctcctc
     2341 aagaccattg gcaagggtaa ttttgccaag gtgaagttgg cccgacacat cctgactggg
     2401 aaagaggtag ctgtgaagat cattgacaag actcaactga actcctccag cctccagaaa
     2461 ctattccgcg aagtaagaat aatgaaggtt ttgaatcatc ccaacatagt taaattattt
     2521 gaagtgattg agactgagaa aacgctctac cttgtcatgg agtacgctag tggcggagag
     2581 gtatttgatt acctagtggc tcatggcagg atgaaagaaa aagaggctcg agccaaattc
     2641 cgccagatag tgtctgctgt gcagtactgt caccagaagt ttattgtcca tagagactta
     2701 aaggcagaaa acctgctctt ggatgctgat atgaacatca agattgcaga ctttggcttc
     2761 agcaatgaat tcacctttgg gaacaagctg gacaccttct gtggcagtcc cccttatgct
     2821 gccccagaac tcttccaggg caaaaaatat gatggacccg aggtggatgt gtggagccta
     2881 ggagttatcc tctatacact ggtcagcgga tccctgcctt ttgatggaca gaacctcaag
     2941 gagctgcggg aacgggtact gagaggaaaa taccgtattc cattctacat gtccacggac
     3001 tgtgaaaacc tgcttaagaa atttctcatt cttaatccca gcaagagagg cactttagag
     3061 caaatcatga aagatcgatg gatgaatgtg ggtcacgaag atgatgaact aaagccttac
     3121 gtggagccac tccctgacta caaggacccc cggcggacag agctgatggt gtccatgggt
     3181 tatacacggg aagagatcca ggactcgctg gtgggccaga gatacaacga ggtgatggcc
     3241 acctatctgc tcctgggcta caagagctcc gagctggaag gcgacaccat caccctgaaa
     3301 ccccggcctt cagctgatct gaccaatagc agcgccccat ccccatccca caaggtacag
     3361 cgcagcgtgt cggccaatcc caagcagcgg cgcttcagcg accaggctgg tcctgccatt
     3421 cccacctcta attcttactc taagaagact cagagtaaca acgcagaaaa taagcggcct
     3481 gaggaggacc gggagtcagg gcggaaagcc agcagcacag ccaaggtgcc tgccagcccc
     3541 ctgcccggtc tggagaggaa gaagaccacc ccaaccccct ccacgaacag cgtcctctcc
     3601 accagcacaa atcgaagcag gaattcccca cttttggagc gggccagcct cggccaggcc
     3661 tccatccaga atggcaaaga cagcacagcc ccccagcgtg tccctgttgc ctccccatcc
     3721 gcccacaaca tcagcagcag tggtggagcc ccagaccgaa ctaacttccc ccggggtgtg
     3781 tccagccgaa gcaccttcca tgctgggcag ctccgacagg tgcgggacca gcagaatttg
     3841 ccctacggtg tgaccccagc ctctccctct ggccacagcc agggccggcg gggggcctct
     3901 gggagcatct tcagcaagtt cacctccaag tttgtacgca ggaacctgaa tgaacctgaa
     3961 agcaaagacc gagtggagac gctcagacct cacgtggtgg gcagtggcgg caacgacaaa
     4021 gaaaaggaag aatttcggga ggccaagccc cgctccctcc gcttcacgtg gagtatgaag
     4081 accacgagct ccatggagcc caacgagatg atgcgggaga tccgcaaggt gctggacgcg
     4141 aacagctgcc agagcgagct gcatgagaag tacatgctgc tgtgcatgca cggcacgccg
     4201 ggccacgagg acttcgtgca gtgggagatg gaggtgtgca aactgccgcg gctctctctc
     4261 aacggggttc gatttaagcg gatatcgggc acctccatgg ccttcaaaaa cattgcctcc
     4321 aaaatagcca acgagctgaa gctttgagca attccgcccc tctccctccc ccccccctaa
     4381 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     4441 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     4501 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     4561 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     4621 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     4681 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     4741 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     4801 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     4861 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     4921 acgatgataa tatggccaca accatgaaga ccttcaacat ctctcagcag gaccttgagc
     4981 tggtggaagt tgccactgag aaaatcacca tgctctatga ggacaacaag caccatgttg
     5041 gtgctgccat caggaccaag acaggagaaa tcatctctgc tgtccacatt gaagcctaca
     5101 ttggcagggt cactgtgtgt gcagaggcca ttgccattgg gtctgcagtc tccaatgggc
     5161 agaaggactt tgacaccatt gtggctgtga ggcaccccta cagtgatgag gtggacaggt
     5221 ccatcagagt ggtgtccccc tgtggcatgt gcagggagct catctcagac tatgcccctg
     5281 attgctttgt tctgattgag atgaatggca agctggtcaa gacaaccatt gaggagctga
     5341 ttccactgaa atacaccaga aactaaacgc gttaagtcga caatcaacct ctggattaca
     5401 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5461 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5521 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5581 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5641 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5701 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5761 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     5821 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5881 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5941 gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc
     6001 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6061 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     6121 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6181 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6241 cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct
     6301 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6361 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6421 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6481 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     6541 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     6601 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     6661 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6721 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6781 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6841 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6901 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6961 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7021 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7081 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7141 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7201 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7261 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     7381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     8341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     9181 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     9241 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     9301 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     9361 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9421 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9481 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagc
//
