LOCUS       sequence-457769-       11379 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral vector for 2xHA LTB4R expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11379)
  AUTHORS   Pedro MP, Lund K, Kang SWS, Chen T, Stuelten CH, Porat-Shliom N, 
            Iglesias-Bartolome R
  TITLE     GPCR Screening Reveals that the Metabolite Receptor HCAR3 Regulates 
            Epithelial Proliferation, Migration, and Cellular Respiration.
  JOURNAL   J Invest Dermatol. 2024 Jun;144(6):1311-1321.e7. doi: 
            10.1016/j.jid.2023.12.002. Epub 2023 Dec 14.
  PUBMED    38103827
REFERENCE   2  (bases 1 to 11379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2023.12.002"; journalName: "J Invest Dermatol"; date:
            "2024-06"; volume: "144"; issue: "6"; pages: "1311-1321.e7"
COMMENT     Sequence Label: pInducer20 2xHA LTB4R
FEATURES             Location/Qualifiers
     source          1..11379
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2780..2806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2810..2836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3955..3979)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4032..4058
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4144..5355
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5192..5211
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5414..6031,6032..6037,6038..6073,6074..6076,6077..6112,
                     6113..6115,6116..6151,6152..6160)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6320..6782
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6376..6393)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6603..6622
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6798..7592
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6798..7592
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6852..6871)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7462..7481
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7769..8357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7822..7842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8240..8251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8432..8665
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8784..8802)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8870..8974
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8975..9043,9044..9835)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9193..9212)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10006..10594
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10495..10514
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10748..10765
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11005..11026
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11041..11046,11047..11064,11065..11071)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11079..11095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11084..11106
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11103..11119
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11103..11119
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11169..11188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctttaaa ggaaccaatt cagtcgactg gatccggtac
     2761 cgctagcgcc gccaccatgt acccatacga tgttcctgac tatgcgggct atccctatga
     2821 cgtcccggac tatgcaggat ccggagatcc acctgtcgca accatgaaca cgactagcag
     2881 cgcggcgcct cctagcctcg gtgttgagtt catatccctg cttgctataa tcctgctttc
     2941 agtagctttg gccgtggggc tgccaggcaa tagctttgtt gtttggtcca ttctcaagag
     3001 aatgcaaaaa cgcagcgtta cggcacttat ggtacttaat ctcgccctcg ccgacctggc
     3061 tgtcctcctc actgcgccct ttttccttca cttccttgcc caaggcacat ggagtttcgg
     3121 acttgcaggg tgccgactct gccactatgt ttgcggcgtc tcaatgtacg cttccgtact
     3181 tcttattact gcgatgagct tggatagaag tttggccgtg gcaaggccgt ttgtgtcaca
     3241 aaagctccgc accaaagcca tggctagaag agtattggcc ggcatttggg tattgtcatt
     3301 tcttctcgct acgcccgtgc ttgcataccg aacagtcgtc ccctggaaaa ctaatatgtc
     3361 tttgtgcttc ccgcgatatc cgagcgaggg tcatagagct tttcatttga tatttgaggc
     3421 ggtcacgggt ttccttttgc cctttctcgc ggtggtcgca tcctactccg atattggtag
     3481 aaggcttcag gcaagaagat tccggcggtc ccgccgcact ggcagattgg tcgtgctgat
     3541 tatacttact ttcgcggcat tctggttgcc ttatcacgtg gtgaacctcg cagaggccgg
     3601 gcgggcattg gccggccagg cagcaggctt gggtttggtc gggaagaggc tgagtcttgc
     3661 tcgcaatgtg ctgattgctt tggcatttct tagcagcagc gtcaatcccg tcttgtacgc
     3721 ttgtgcgggc ggtggacttc tgcggtcagc cggcgttgga tttgtggcaa aactcctcga
     3781 aggaaccggt tccgaggcat caagcacgag gagagggggg tctttgggtc aaaccgcacg
     3841 ctccggcccg gcagcactcg aacctggacc ctctgaaagc ttgactgcga gcagtcctct
     3901 taagctgaat gaactgaatt agtaagaatt cgcggccgca ctcgagatat ctagacccag
     3961 ctttcttgta caaagtggtt gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4021 cgcggttcga atacccatac gacgtcccag actacgctta gtaatgatta attaaactag
     4081 aaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagtaac ccacccaaga
     4141 tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg
     4201 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     4261 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     4321 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     4381 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     4441 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     4501 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     4561 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     4621 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     4681 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     4741 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     4801 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     4861 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     4921 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     4981 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     5041 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     5101 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     5161 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     5221 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     5281 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     5341 gcttttttgt tagacgaagc ttggtaccga gctcggatct ccaccccgta ccggtcctgc
     5401 agtcgaattc accatgtcta gactggacaa gagcaaagtc ataaacggag ctctggaatt
     5461 actcaatggt gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt
     5521 tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc
     5581 aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg agtcatggca
     5641 agactttctg cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg
     5701 ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca
     5761 gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc
     5821 cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga
     5881 ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag caattgagct
     5941 gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg
     6001 cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga
     6061 cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga
     6121 cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg atccgcggcc
     6181 gcactagagg aattccgccc ctctcccccc cccnncccyc ccccccccct aacgttactg
     6241 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     6301 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     6361 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     6421 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     6481 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     6541 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     6601 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     6661 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     6721 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     6781 aagcttaccg gtccaccatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6841 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6901 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6961 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7021 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7081 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7141 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7201 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7261 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7321 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7381 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7441 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7501 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7561 ccttctatcg ccttcttgac gagttcttct gatgtacaag taaagcggcc gcgactctag
     7621 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     7681 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgtttagtc cctcccaatt
     7741 cgatatcaag cttatcgcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     7801 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     7861 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     7921 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7981 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     8041 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     8101 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     8161 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     8221 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     8281 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     8341 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     8401 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     8461 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     8521 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8581 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8641 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     8701 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     8761 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     8821 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     8881 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     8941 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     9001 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     9061 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     9121 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     9181 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     9241 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     9301 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     9361 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     9421 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     9481 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     9541 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     9601 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     9661 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     9721 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     9781 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     9841 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     9901 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     9961 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    10021 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    10081 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    10141 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    10201 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    10261 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10321 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10381 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10441 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10501 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10561 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10621 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10681 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10741 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    10801 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
    10861 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10921 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
    10981 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    11041 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    11101 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
    11161 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
    11221 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
    11281 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
    11341 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
