LOCUS       sequence-457775-        6070 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of WT Rab6a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: PCMV-intron myc Rab6WT
FEATURES             Location/Qualifiers
     source          1..6070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(18..36)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(19..36)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(56..73)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    62..286
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      332..760
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(419..438)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     629..650
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(769..789)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        774..1103
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      954..1089
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1016..1035
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1170..1964
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1224..1243)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1834..1853
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2138..2259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2175..2194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2229..2248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2308..2324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2308..2324)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2321..2343)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2332..2348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2356..2362,2363..2380,2381..2386))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2401..2422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2539..2556)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2710..3298)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2790..2809)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3469..4260,4261..4329))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4092..4111
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4330..4434)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4807..5018
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4969..4989
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5015..5039
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          5179..5311
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5294..5313
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        5356..5374
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5389..5418
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
       61 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
      121 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
      181 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
      241 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
      301 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
      361 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      421 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      481 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      541 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      601 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      661 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      721 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
      781 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      841 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      901 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      961 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1021 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     1081 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     1141 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     1201 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     1261 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     1321 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     1381 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     1441 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     1501 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     1561 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     1621 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     1681 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     1741 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     1801 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     1861 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     1921 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     1981 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     2041 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     2101 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     2161 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2221 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     2281 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     2341 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     2401 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     2461 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     2521 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2581 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2641 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2701 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2761 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2821 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2881 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2941 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3001 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3061 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3121 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3181 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3241 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3301 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3361 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3421 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3481 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3541 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3601 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3661 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3721 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3781 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3841 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3901 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3961 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4021 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4081 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4141 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4201 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4261 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4321 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4381 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     4441 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cttcaatatt ggccattagc
     4501 catattattc attggttata tagcataaat caatattggc tattggccat tgcatacgtt
     4561 gtatctatat cataatatgt acatttatat tggctcatgt ccaatatgac cgccatgttg
     4621 gcattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     4681 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggca ttatgcccag
     4741 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4801 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
     4861 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4921 cgggactttc caaaatgtcg taacaactgc gatcgcccgc cccgttgacg caaatgggcg
     4981 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatca
     5041 ctagaagctt tattgcggta gtttatcaca gttaaattgc taacgcagtc agtgcttctg
     5101 acacaacagt ctcgaactta agctgcagtg actctcttaa ggtagccttg cagaagttgg
     5161 tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag
     5221 aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt
     5281 actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct
     5341 taaggctaga gtacttaata cgactcacta taggctagcc tcgacatgga acagaaactc
     5401 atctctgaag aggatctgtc tagaggatcc accatgtcca cgggcggaga cttcgggaat
     5461 ccgctgagga aattcaagct ggtgttcctg ggggagcaaa gcgttggaaa gacatctttg
     5521 atcaccagat tcatgtatga cagttttgac aacacctatc aggcaacaat tggcattgac
     5581 tttttatcaa aaactatgta cttggaggat cgaacagtac gattgcaatt atgggacaca
     5641 gcaggtcaag agcggttcag gagcttgatt cctagctaca ttcgtgactc cactgtggca
     5701 gttgttgttt atgatatcac aaatgttaac tcattccagc aaactacaaa gtggattgat
     5761 gatgtcagaa cagaaagagg aagtgatgtt atcatcatgc tagtaggaaa taaaacagat
     5821 cttgctgaca agaggcaagt gtcaattgag gagggagaga ggaaagccaa agagctgaat
     5881 gttatgttta ttgaaactag tgcaaaagct ggatacaatg taaagcagct ctttcgacgt
     5941 gtagcagcag ctttgccggg aatggaaagc acacaggaca gaagcagaga agatatgatt
     6001 gacataaaac tggaaaagcc tcaggagcaa ccagtcagtg aaggaggctg ttcctgctaa
     6061 ctcgagcatg
//
