LOCUS       sequence-457965-       10448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 BA.5
FEATURES             Location/Qualifiers
     source          1..10448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(221..423)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(250..270)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        425..728
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    809..857
                     /note="synthetic polyadenylation signal"
     terminator      875..921
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    926..997
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1149..1285)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1298..2089,2090..2158))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1921..1940
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2904..3492
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3393..3412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3688..3912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(3923..4717)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4034..4053)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4644..4663
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4731..4927)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4745..4880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    4972..5043
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(5088..5224)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(5254..5300)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    5415..5536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5452..5471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5506..5525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5589..6177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5695..5706
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6104..6124
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     intron          join(10060..10448,1..87)
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 taccctgtta cttctcccct tcctatgaca tgaacttaac catagaaaag aaggggaaag
       61 aaaacatcaa gggtcccata gactcaccct gaagttctca ggatccgagc tcggtaccac
      121 atgtaagctt cgaggggagg ctggatcggt cccggtgtct tctatggagg tcaaaacagc
      181 gtggatggcg tctccaggcg atctgacggt tcactaaacc agctctgctt atatagacct
      241 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
      301 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
      361 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
      421 tcagcatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
      481 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
      541 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
      601 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
      661 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
      721 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactaga
      781 tctggctaca ctcgatcagc agttagataa taaaatcgct atccatcgaa gatggatgtg
      841 tgttggtttt ttgtgtgtgt aacgcaacga ttgatagcat aaccccttgg ggcctctaaa
      901 cgggtcttga ggggtttttt gaattgagag gtagctgaag ctgctgttat gaccactaga
      961 gggaagaaga tacctgtgga gctaatggtc caagatgtcg ggagccgcga tcgctgccaa
     1021 aaaggtaagc tgacgatagc tgacgagatt cgatgcccga cgagcaaatg agcgccacgg
     1081 ctgttcgcgt aagctcgaaa cgacggagat tcgatagagc cagagtcgag tttaaacgat
     1141 tcgggatctt aaggataaat ttctggtaag gaggacacgt atggaagtgg gcaagttggg
     1201 gaagccgtat ccgttgctga atctggcata tgtgggagta taagacgcgc agcgtcgcat
     1261 caggcatttt tttctgcgcc aatgcaccta gaatcggtta ccaatgctta atcagtgagg
     1321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     1381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     1441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     1501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tatcaactgt tgccgggaag
     1561 ctagagtaag tagttcgcca gttaatagtt tacgcaaggt tgttgccatt gctacaggca
     1621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     1681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccaa
     1741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     1801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgaa tactcaacca
     1861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     1921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaag cgttcttcgg
     1981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgcg
     2041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatgc
     2161 gaaagcctcc tgctgatcct gtctcttgat agatgtttgc aaaagctagg actcgattat
     2221 acgttgaccg atcagactat caatatctag gtttaaacag atcggtacgc tgccgtgacc
     2281 aggcttgagt acaatatctg acgattggac ttcctgggtc ccaagctgga ttccgactgg
     2341 ctgactgcca agcaacgata tggatcgtcg agactcgcac tgagaggatc gttctcggta
     2401 caccaggcgc gccccgattt cgatattcct cggatctcgg atagatcggg attgaggatg
     2461 taggttgaac ttctctcggg tgatcccgat ctcggatatt cgggattgga gtaatacctg
     2521 gcgataggcg ctgccaagca atagccaagg cgcaaggccg gccaagagac ttgcgatagt
     2581 tcgttcgaga ggtagcctgc aagatggatc ggacgaaagg tttgaagatt ttccgacgag
     2641 ggccttggag agttcggaaa tttaaatgca actatggatg aacgaaatag acagatcgct
     2701 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2761 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2821 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2881 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2941 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3001 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3061 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3121 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3181 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3241 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     3301 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3361 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3421 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3481 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3541 tttgctcaca tgatttaaat gaaactcgta atcggagaac gggtgcaata gcaaggacca
     3601 gatcgaactt gactgacgca attctgagcg tagtcgaaac ccagacaagt cgatctcgcc
     3661 aagtagctga cttgactggt taattaacca tagagcccac cgcatcccca gcatgcctgc
     3721 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     3781 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     3841 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     3901 tagaaggcac agggcttaca tactagaaga actcgtcaag aaggcgatag aaggcgatgc
     3961 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     4021 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     4081 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     4141 agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatccgc gccttgagcc
     4201 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     4261 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     4321 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     4381 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     4441 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg
     4501 tcgtggccag ccacgatagc cgcgctgcct cgtcctggag ttcattcagg gcaccggaca
     4561 ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat
     4621 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg
     4681 cgggagaacc tgcgtgcaat ccatcttgtt caatcatgtt ggctacctcc tttgcaaaag
     4741 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     4801 tcggcctctg cataaataaa aaaaattagt cagcgatggg gcggagaatg ggcggaactg
     4861 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     4921 agatgcagat cgcttaatta agtttcgata gcccaaggta accaatgaga ttagggaggg
     4981 aagaaagttt gatgaactac ttctgacccc tagtggtgtc cagaaaagac cattaaagga
     5041 atgtcttgat cgaaatcgag agatcgcctg caggatagct gacgacctta aggataaatt
     5101 tctggtaagg aggacacgta tggaagtggg caagttgggg aagccgtatc cgttgctgaa
     5161 tctggcatat gtgggagtat aagacgcgca gcgtcgcatc aggcattttt ttctgcgcca
     5221 atgccggtag atagcaatcc ggcccgaggg gcacaaaaaa cccctcaaga cccgtttaga
     5281 ggccccaagg ggttatgcta cgataccgat agagatgggc caataccaca tttgtagagg
     5341 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     5401 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5521 tcatcaatgt atcttatcat gtctgtgagc tgaaggtacg ctgtatctca gtcagtcaag
     5581 ctagacaggc ggggaggcgg cccaaaggga gatccgactc gtctgagggc gaaggcgaag
     5641 acgcggaaga ggccgcagag ccggcagcag gccgcgggaa ggaaggtccg ctggattgag
     5701 ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc caggtggcaa cacaggcgag
     5761 cagccatgga aaggacgtca gcttccccga caacaccacg gaattgtcag tgcccaacag
     5821 ccgagcccct gtccagcagc gggcaaggca ggcggcgatg agttccgccg tggcaatagg
     5881 gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt
     5941 gggggttgcg tcagcaaaca cagtgcacac cacgccacgt tgcctgacaa cgggccacaa
     6001 ctcctcataa agagacagca accaggattt atacaaggag gagaaaatga aagccatacg
     6061 ggaagcaata gcatgataca aaggcattaa agcagcgtat ccacatagcg taaaaggagc
     6121 aacatagtta agaataccag tcaatctttc acaaattttg taatccagag gttgattagg
     6181 cctgcggatc cagaaatcga ttgtatcagt cagtcagtgc aggaggagac aacttctaga
     6241 ggatccttta tttacaacaa gatccgcaac tgcaacatcc tttcaagcag gaacaacagc
     6301 tagtcataca gcacaacata attgtgacca taacgatagc tataagtccg gcgataaagc
     6361 ccaaccaaat gtaccagggc catttaatat attgttcgta tttgcccagt tcttgcaagt
     6421 cgatcaaaga ttcattcaag ttcttggcga cctcattaag gcgatcaatc tctttctgaa
     6481 tgttgacgac acttgcattt attcctgata tgtccccgag atcaacatcg ggtgaagtat
     6541 gattcttaaa gtatttatcc aattcctctt tgaagctatc caactcgggt tgcaggggat
     6601 catataccgt gttgttcacg atcccaatga ccacatcaca gttgcctgag acaaatgtat
     6661 tgtcagtggt tatgatttgg ggttcataga aattcctttg ggtcacaaac caatgcgtgc
     6721 cattactgac gaaaacgcct tccctgggaa agtgcgcttt gccatcgtga cagattgctg
     6781 gagctgtcgt aaagttcttc tcttgagcag ggacataagt gacgtggaga aagaccactc
     6841 catgtggggc ggattggggg aaggacatca agtgatagcc cttgccacaa aagtcaaccc
     6901 gtttggactg cccgagcacg cactctgaca ttttagtggc ggccaggttg gcgcttgctc
     6961 gtatctcagc agcccggatc aactgttgtg tcacataagt ttggagtgac tggagcctgc
     7021 ctgtgatgag ccgatcaatt tgcacctcgg cttccacctt atcaagtctg gacaatatgt
     7081 cgttgaggac gctggagatt gcgccgaatt tagaactcag ttgcttcacg agggtattca
     7141 gggcctgggc gttatgattc acaacatctt ggagtttgcc gagggcggac gctgtgcttg
     7201 acagtgaatc ttggatcttg ccaatcgcgc tgttaaattg attagcgatc agcttctgat
     7261 tttcatacaa tacattttgc gtgacaccaa tcccattgaa tctgtaagcc atttgcatag
     7321 cgaaaggtat ttgaagggcc gcacccgcgc caaaagtcca gccggatgta atggttcctg
     7381 ccaacaatgc tgatgtatac tgtgcaatca tctcatcagt gagcagaggg ggcagaactg
     7441 tcaacccatt gaatttttga gcacagataa ggtcgcgggc cgcaatgtct cccaaacaat
     7501 ccccatactg cttaatgaaa cccgcgtcag ccaacgtaac tttgttgaac agcagatcct
     7561 cgataaagct gcgttttgaa ggcttagatg ggtctggcag tatttggcta aaattaaacc
     7621 cgccaaaata tttgattggc ggcgttttgt atatttgctt tacttgtgcg aacacctctt
     7681 gggtattctt gtcttgttcg accgcaattc cggtcaatgc tcgtttgagt tgagtacaga
     7741 aagacccgta ttgaagcaag aggttgctac actcggtgga atccccacag atatacatcg
     7801 tacagtctac gctcgtctta gtcatgctga cgggcaagat ttccgtggta acgctaatgg
     7861 taaagttagt tgggattgcg atggaattgt ttgaataagc cacggagttc tcagccccga
     7921 gactcatggt ataggcgatt atggactggc tggctactga gcgagcgcgc cggtgtgatt
     7981 tcgtttgagt ttgataagac gcacagattc ctgccccaat aggaatatca cattcgtatg
     8041 aattattgac atactcagcg ccaatgagac aacccgctct agtttgaaag acattggaac
     8101 ctgttgagta gactctccag gtgggtgtca gttggtcggc gtgtatcgcc acagggactt
     8161 cggtacagtt gacaccttga tacaggacag ccacttggtt ggatgtgttc gttccgggag
     8221 ttatcacgct gaccccgccg aagctacagg gtgttatatc gaggatttcc agtgtttgcg
     8281 gatcgcggac ggcatctgtg gtatcggcaa tgtctctacc aaattgttga aaagggagaa
     8341 atttcttgtt tgattctgtc agcacccctg ttccggtgag cccattaaag ttgaagttca
     8401 cacatttatt cttcaccagg ttagtgcttt tcttcggtcc gcaaactgta gccggtgcgt
     8461 ggagcagctc aaagctcaag actaccaccc gatatggttg atggcccacc ccatatgttg
     8521 gtcgaaatcc gtatgattga aggggaaaat aacagttaac ccctgccact ccattacatg
     8581 gtttgttgcc agcttgataa atttcggtgg agatgtcacg ttcaaagggc ttcagatttg
     8641 acttgcgaaa caggcggtaa cgataattat agttgccgcc gacttttgaa tcgagcttat
     8701 tggagttcca tgctataacg cagccggtga agtcatccgg caacttataa ttatagtctg
     8761 ctatatttcc ggtttgcccc ggtgcaatct ggctgacttc gttgccgcgt atgacgaaag
     8821 aatctgcgta aacgttggta aaacacaagt cattcaattt cgtagggctg accccataac
     8881 acttaaaagc gaagaatggt gcgaaattat acaggacact ataatctgca acacaatttg
     8941 atattcgctt gcgattccag gcataaactg atgcaaatcg agtggcatta aatacttcat
     9001 cgaaaggaca caaattggtg atgttaggga atcgaacaat ggattcggtg ggttggacgc
     9061 ggaagttact ggtttggtat atgcctttct caactgtgaa ggacttgagt gtgcatttag
     9121 tttcggacag gggatcgagg gcacagtcta cggcatcggt aattgttccg ttttcgttat
     9181 atttgagaag aaaggtgcgt ggttgcaaat atcccacata gtaggctgct gcgcccgctg
     9241 tccaaccact actactgtcg ccaggcgtga ggtagcttcg atgaagggca agaagggttt
     9301 ggaaccgggt gatgttgatg ccaatcggca agtccacgag gggttccaac gcggagaagc
     9361 cttgagggag atcccgaccc agatttatcg gggtatgctt gctataaatt ttgaagtagc
     9421 cgtctatatt cttaaacaca aattcgcgga gattcttgaa gttgccttgc ttcccttcaa
     9481 gatccatgag aaatggttgg gaaacgtact caaaagtaca gttgttagcg gaactgtaaa
     9541 cgcggaactc tgactccatc caagacttgt tattcttatg ataataaaca tcgagaaagg
     9601 gatcgttaca aaattgaaat tcacagactt tgatgacgac attggtggcg ttgttcacaa
     9661 tgaggagaga ctgtgtcttg gagtccaggg tggtgccaaa tatccatccc cggataatgt
     9721 tggacttctc cgttgaggca aagtatacgc catcgttaaa tggcaagacg ggattatcaa
     9781 atcgtttagt tccgttggtg ccagagatag catggaacca ggtgacattt gagaagaatg
     9841 gcaggaaaag gtcttgagta gaatgaagga cactggacct aaagacttta tcgggatagt
     9901 atactccacg agtaaaagag ttcgtgtaag actgcgttct agttatgagg ttcacgcatt
     9961 gggaagaaac gaggggcagg agaacgagga acacaaacat ggccggccgc ctttgccaaa
    10021 gtgatgggcc agcacacaga ccagcacgtt gcccaggagc tgtgggagga agataagagg
    10081 tatgaacatg attagcaaaa gggcctagct tggactcaga ataatccagc cttatcccaa
    10141 ccataaaata aaagcagaat ggtagctgga ttgtagctgc tattagcaat atgaaacctc
    10201 ttacatcagt tacaatttat atgcagaaat atttatatgc agaaatattg ctattgcctt
    10261 aacccagaaa ttatcactgt tattctttag aatggtgcaa agaggcatga tacattgtat
    10321 cattattgcc ctgaaagaaa gagattaggg aaagtattag aaataagata aacaaaaaag
    10381 tatattaaaa gaagaaagca ttttttaaaa ttacaaatgc aaaattaccc tgatttggtc
    10441 aatatgtg
//
