LOCUS       sequence-458039-        2712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2712)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG/VPS34(R561D|R566D|K567D|K568D)
FEATURES             Location/Qualifiers
     source          1..2712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gatcgacggt atcgatagcc accatgggcg aggctgaaaa gttccattac atctattcct
       61 gcgacctgga tatcaacgtg cagctgaaga ttggatccct ggagggcaaa cgggaacaga
      121 aatcttataa ggccgtgctg gaggacccca tgctgaagtt tagcggactg tatcaggaaa
      181 catgctccga tctgtacgtg acttgtcagg tgttcgctga gggaaagcca ctggctctgc
      241 ctgtgaggac ctcctacaaa gccttttcta caagatggaa ctggaatgaa tggctgaaac
      301 tgcctgtgaa gtatcctgac ctgccaagaa acgcccaggt ggctctgacc atctgggatg
      361 tgtacggacc tggaaaggct gtgccagtgg gagggaccac agtgtccctg ttcgggaagt
      421 acggaatgtt tcggcagggg atgcacgacc tgaaagtgtg gcccaatgtg gaggccgatg
      481 gctctgaacc aactaagacc cccgggagaa caagctccac tctgagtgag gaccagatgt
      541 caaggctggc caaactgact aaggctcacc gccagggaca tatggtgaag gtggactggc
      601 tggatcggct gaccttcaga gagatcgaaa tgattaacga gagcgaaaaa cggtctagta
      661 atttcatgta tctgatggtg gagtttagat gcgtgaaatg tgacgataag gaatacggaa
      721 tcgtgtacta tgagaaggac ggcgatgaat caagccccat tctgaccagc tttgagctgg
      781 tgaaagtgcc agacccccag atgagtatgg agaacctggt ggaatcaaaa caccataagc
      841 tggcccggtc cctgagatct ggccctagtg accatgatct gaagccaaac gccgctacaa
      901 gggaccagct gaatatcatt gtgagctatc cccctactaa acagctgacc tacgaggaac
      961 aggatctggt gtggaagttc cgctactatc tgactaacca ggagaaagcc ctgaccaaat
     1021 ttctgaagtg cgtgaattgg gacctgcctc aggaggccaa gcaggctctg gaactgctgg
     1081 gcaaatggaa gccaatggac gtggaggata gcctggaact gctgtcctct cactatacca
     1141 atccaacagt gcggagatac gctgtggcta ggctgaggca ggctgacgat gaggatctgc
     1201 tgatgtatct gctgcagctg gtgcaggccc tgaagtacga gaacttcgac gatatcaaaa
     1261 atgggctgga acccaccaag aaagacagcc agagttcagt gtccgagaac gtgtcaaata
     1321 gcggaatcaa cagcgctgaa attgatagct cccagatcat tacctcacct ctgccaagcg
     1381 tgtctagtcc accccctgcc tccaaaacaa aggaggtgcc tgacggcgag aatctggaac
     1441 aggatctgtg caccttcctg atctcacggg cctgtaagaa cagcacactg gctaattacc
     1501 tgtattggta cgtgattgtg gagtgtgaag accaggatac acagcagaga gacccaaaga
     1561 ctcatgagat gtacctgaac gtgatgaggc gcttcagcca ggccctgctg aaaggggaca
     1621 agtccgtgag ggtgatgcgc tctctgctgg ccgctcagca gacctttgtg gatcggctgg
     1681 tgcacctgat gaaggctgtg caggacgagt ctgggaacga tgacgataag aatgaaagac
     1741 tgcaggccct gctgggagac aacgagaaaa tgaatctgag tgatgtggag ctgatccccc
     1801 tgcctctgga accccaggtg aagattagag gaatcattcc tgagacagcc actctgttca
     1861 aatccgccct gatgccagct cagctgttct ttaaaacaga agacggcggc aagtatcccg
     1921 tgatctttaa acatggcgac gatctgaggc aggatcagct gattctgcag atcatttctc
     1981 tgatggacaa actgctgcgc aaggagaacc tggatctgaa actgacaccc tacaaggtgc
     2041 tggctaccag tacaaagcac ggcttcatgc agtttatcca gtcagtgcct gtggccgagg
     2101 tgctggacac tgaagggagc attcagaatt tctttaggaa gtatgccccc tctgagaacg
     2161 gccctaatgg gatcagtgct gaagtgatgg atacttacgt gaaaagctgc gccggctatt
     2221 gtgtgatcac ctacattctg ggagtgggcg acaggcatct ggataacctg ctgctgacta
     2281 aaaccgggaa gctgttccac atcgactttg ggtatattct gggaagggat ccaaagccac
     2341 tgccaccacc tatgaaactg aataaggaga tggtggaagg catgggggga acacagtccg
     2401 agcagtatca ggaattccgc aagcagtgtt acactgcctt tctgcacctg cggagatact
     2461 caaacctgat cctgaatctg ttcagcctga tggtggacgc caatatccca gatattgctc
     2521 tggagcccga caagaccgtg aaaaaggtgc aggataaatt taggctggac ctgtctgatg
     2581 aggaagccgt gcactatatg cagtctctga tcgacgagag tgtgcatgct ctgttcgccg
     2641 ctgtggtgga acagattcac aagtttgccc agtactggcg caaatgactc gaggaattca
     2701 ctcctcaggt gc
//
