LOCUS       sequence-458040-        2819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2819)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG/TSF-VPS34
FEATURES             Location/Qualifiers
     source          1..2819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..59
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             96..119
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             126..149
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcgatcga cggtatcgat gccaccatgg ctagctggag ccacccgcag ttcgagaaag
       61 gtggaggtgc ccgaggtgga tcgggaggtg gatcgtggag ccacccgcag ttcgaaaaag
      121 gattcgatta caaggatgac gatgacaagg gtaccatggg cgaggctgaa aagttccatt
      181 acatctattc ctgcgacctg gatatcaacg tgcagctgaa gattggatcc ctggagggca
      241 aacgggaaca gaaatcttat aaggccgtgc tggaggaccc catgctgaag tttagcggac
      301 tgtatcagga aacatgctcc gatctgtacg tgacttgtca ggtgttcgct gagggaaagc
      361 cactggctct gcctgtgagg acctcctaca aagccttttc tacaagatgg aactggaatg
      421 aatggctgaa actgcctgtg aagtatcctg acctgccaag aaacgcccag gtggctctga
      481 ccatctggga tgtgtacgga cctggaaagg ctgtgccagt gggagggacc acagtgtccc
      541 tgttcgggaa gtacggaatg tttcggcagg ggatgcacga cctgaaagtg tggcccaatg
      601 tggaggccga tggctctgaa ccaactaaga cccccgggag aacaagctcc actctgagtg
      661 aggaccagat gtcaaggctg gccaaactga ctaaggctca ccgccaggga catatggtga
      721 aggtggactg gctggatcgg ctgaccttca gagagatcga aatgattaac gagagcgaaa
      781 aacggtctag taatttcatg tatctgatgg tggagtttag atgcgtgaaa tgtgacgata
      841 aggaatacgg aatcgtgtac tatgagaagg acggcgatga atcaagcccc attctgacca
      901 gctttgagct ggtgaaagtg ccagaccccc agatgagtat ggagaacctg gtggaatcaa
      961 aacaccataa gctggcccgg tccctgagat ctggccctag tgaccatgat ctgaagccaa
     1021 acgccgctac aagggaccag ctgaatatca ttgtgagcta tccccctact aaacagctga
     1081 cctacgagga acaggatctg gtgtggaagt tccgctacta tctgactaac caggagaaag
     1141 ccctgaccaa atttctgaag tgcgtgaatt gggacctgcc tcaggaggcc aagcaggctc
     1201 tggaactgct gggcaaatgg aagccaatgg acgtggagga tagcctggaa ctgctgtcct
     1261 ctcactatac caatccaaca gtgcggagat acgctgtggc taggctgagg caggctgacg
     1321 atgaggatct gctgatgtat ctgctgcagc tggtgcaggc cctgaagtac gagaacttcg
     1381 acgatatcaa aaatgggctg gaacccacca agaaagacag ccagagttca gtgtccgaga
     1441 acgtgtcaaa tagcggaatc aacagcgctg aaattgatag ctcccagatc attacctcac
     1501 ctctgccaag cgtgtctagt ccaccccctg cctccaaaac aaaggaggtg cctgacggcg
     1561 agaatctgga acaggatctg tgcaccttcc tgatctcacg ggcctgtaag aacagcacac
     1621 tggctaatta cctgtattgg tacgtgattg tggagtgtga agaccaggat acacagcaga
     1681 gagacccaaa gactcatgag atgtacctga acgtgatgag gcgcttcagc caggccctgc
     1741 tgaaagggga caagtccgtg agggtgatgc gctctctgct ggccgctcag cagacctttg
     1801 tggatcggct ggtgcacctg atgaaggctg tgcagaggga gtctgggaac cgcaagaaaa
     1861 agaatgaaag actgcaggcc ctgctgggag acaacgagaa aatgaatctg agtgatgtgg
     1921 agctgatccc cctgcctctg gaaccccagg tgaagattag aggaatcatt cctgagacag
     1981 ccactctgtt caaatccgcc ctgatgccag ctcagctgtt ctttaaaaca gaagacggcg
     2041 gcaagtatcc cgtgatcttt aaacatggcg acgatctgag gcaggatcag ctgattctgc
     2101 agatcatttc tctgatggac aaactgctgc gcaaggagaa cctggatctg aaactgacac
     2161 cctacaaggt gctggctacc agtacaaagc acggcttcat gcagtttatc cagtcagtgc
     2221 ctgtggccga ggtgctggac actgaaggga gcattcagaa tttctttagg aagtatgccc
     2281 cctctgagaa cggccctaat gggatcagtg ctgaagtgat ggatacttac gtgaaaagct
     2341 gcgccggcta ttgtgtgatc acctacattc tgggagtggg cgacaggcat ctggataacc
     2401 tgctgctgac taaaaccggg aagctgttcc acatcgactt tgggtatatt ctgggaaggg
     2461 atccaaagcc actgccacca cctatgaaac tgaataagga gatggtggaa ggcatggggg
     2521 gaacacagtc cgagcagtat caggaattcc gcaagcagtg ttacactgcc tttctgcacc
     2581 tgcggagata ctcaaacctg atcctgaatc tgttcagcct gatggtggac gccaatatcc
     2641 cagatattgc tctggagccc gacaagaccg tgaaaaaggt gcaggataaa tttaggctgg
     2701 acctgtctga tgaggaagcc gtgcactata tgcagtctct gatcgacgag agtgtgcatg
     2761 ctctgttcgc cgctgtggtg gaacagattc acaagtttgc ccagtactgg cgcaaatga
//
