LOCUS       sequence-458043-        4285 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4285)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCAG/VPS15(R36D)-TSF
FEATURES             Location/Qualifiers
     source          1..4285
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4103..4123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4136..4159
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4196..4219
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4226..4249
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gatcgacggt atcgaagcca ccatgggaaa tcagctggcc ggcatcgctc ctagccagat
       61 tctgtccgtg gaatcttact tcagtgacat ccatgatttt gagtatgaca aatcactggg
      121 aagcaccgac ttctttaagg tggcccgggc taaacacaga gaaggcctgg tggtggtgaa
      181 ggtgttcgct atccaggatc ccactctgcc tctgaccagt tataaacagg agctggagga
      241 actgaagatt agactgaact cagcccagaa ttgcctgcca ttccagaaag ctagcgagaa
      301 ggcctccgaa aaagccgcta tgctgtttag gcagtacgtg cgcgacaacc tgtatgatcg
      361 gatttccaca agacccttcc tgaacaatat cgagaagcgg tggatcgcct ttcagattct
      421 gactgctgtg gaccaggccc ataaatctgg agtgagacac ggcgatatca agactgagaa
      481 cgtgatggtg accagctgga attgggtgct gctgacagac ttcgcttcct ttaagccaac
      541 ttacctgccc gaagacaacc ctgccgattt caattatttc tttgacacta gccggagaag
      601 gacctgctac atcgctcctg agcggttcgt ggatggcggg atgtttgcca ccgagctgga
      661 atacatgaga gacccatcaa cacccctggt ggatctgaac agcaatcaga ggaggagagg
      721 agagctgaag agggctatgg acatcttttc tgctgggtgt gtgattgccg agctgttcac
      781 cgaaggagtg cctctgtttg atctgagtca gctgctggcc tataggaacg gacacttctt
      841 tccagagcag gtgctgaata agatcgagga tcatagcatt cgcgaactgg tgacacagat
      901 gatccacagg gaaccagaca aacgcctgga ggctgaagat tacctgaagc agcagagagg
      961 caacgccttc ccagagatct tctacacatt tctgcagccc tatatggccc agttcgctaa
     1021 agagactttt ctgagcgccg acgaacggat cctggtcatc agaaaggatc tgggaaacat
     1081 cattcataat ctgtgcggcc acgacctgcc cgaaaaagcc gagggcgaac ctaaggagaa
     1141 tgggctggtc atcctggtgt ctgtgattac aagttgcctg cagactctga agtattgtga
     1201 tagcaaactg gccgctctgg aactgatcct gcatctggct ccaaggctgt ccgtggagat
     1261 cctgctggac agaattaccc cttacctgct gcacttctcc aacgattctg tgccacgggt
     1321 gagagccgag gctctgagga cactgactaa agtgctggcc ctggtgaagg aagtgcctcg
     1381 caacgacatc aatatctacc ccgagtatat cctgcctggc attgcccacc tggctcagga
     1441 cgatgccact attgtgaggc tggcctatgc tgagaatatc gccctgctgg ctgaaaccgc
     1501 cctgcgcttt ctggagctgg tgcagctgaa gaacctgaat atggaaaacg accccaacaa
     1561 tgaggaaatc gatgaggtga cccatcctaa cgggaattac gacacagagc tgcaggccct
     1621 gcacgaaatg gtgcagcaga aagtggtgac cctgctgtcc gatccagaaa acattgtgaa
     1681 gcagactctg atggagaatg ggatcaccag gctgtgtgtg ttctttggac gccagaaagc
     1741 taacgacgtg ctgctgtcac atatgatcac attcctgaac gacaagaatg attggcacct
     1801 gcggggcgcc ttctttgata gcatcgtggg agtggccgct tatgtgggct ggcagagctc
     1861 ctctattctg aagcctctgc tgcagcaggg gctgtctgac gccgaggaat ttgtgatcgt
     1921 gaaagctctg tatgccctga cctgcatgtg tcagctggga ctgctgcaga agccacatgt
     1981 gtacgagttc gctagcgata tcgccccatt tctgtgccac cccaatctgt ggattcggta
     2041 tggggccgtg ggattcatca ctgtggtggc tagacagatt agcaccgccg acgtgtactg
     2101 taaactgatg ccatacctgg atccctatat cacacagccc atcattcaga ttgaacggaa
     2161 actggtgctg ctgtccgtgc tgaaggagcc tgtgtctagg agtattttcg actacgccct
     2221 gcgcagcaag gatatcactt ccctgtttag acacctgcat atgaggcaga agaaacgcaa
     2281 cggatccctg cctgactgcc cacctccaga ggatccagcc atcgctcagc tgctgaagaa
     2341 actgctgtct caggggatga ccgaggaaga ggaagacaag ctgctggccc tgaaagattt
     2401 catgatgaag tctaacaagg ccaaagctaa tatcgtggac cagagtcacc tgcatgatag
     2461 ttcacagaag ggcgtgatcg acctggccgc tctgggcatt acagggaggc aggtggatct
     2521 ggtgaagact aaacaggagc ccgacgataa aagggcccgc aagcacgtga aacaggacag
     2581 caacgtgaat gaggaatgga agtcaatgtt tggaagcctg gaccccccta atatgcctca
     2641 ggctctgcca aaaggcagcg atcaggaagt gatccagacc gggaagccac caagatcaga
     2701 gagctccgcc ggcatttgtg tgcccctgag cacatctagt caggtgcctg aggtgaccac
     2761 agtgcagaac aagaaacctg tgatcccagt gctgtcaagc accattctgc caagcacata
     2821 ccagatcagg attactacct gcaaaaccga actgcagcag ctgatccagc agaagcggga
     2881 gcagtgtaac gctgaaagaa ttgccaagca gatgatggag aatgccgagt gggaaagtaa
     2941 acctccacca cctggatgga ggccaaaggg actgctggtg gctcacctgc atgaacacaa
     3001 aagcgccgtg aatcggatca gagtgtccga cgagcactct ctgttcgcca catgctccaa
     3061 cgatggaact gtgaagattt ggaattctca gaagatggag ggcaaaacaa ctaccacaag
     3121 gtctatcctg acctatagtc ggattggagg cagagtgaag accctgacat tttgtcaggg
     3181 cagccattac ctggccatcg cttccgacaa cggggctgtg cagctgctgg gaattgaagc
     3241 ctcaaagctg cccaaaagcc ctaagatcca ccccctgcag tccaggattc tggaccagaa
     3301 ggaggatggc tgcgtggtgg acatgcacca tttcaacagt ggggcccagt cagtgctggc
     3361 ctacgctaca gtgaatgggt ctctggtggg atgggatctg cgctcctcta gtaacgcttg
     3421 gactctgaag catgacctga aatccggcct gatcacctct ttcgccgtgg atattcacca
     3481 gtgctggctg tgtatcggca catcaagcgg gactatggct tgctgggaca tgcgctttca
     3541 gctgccaatc tcctctcatt gtcacccaag cagggctagg attaggaggc tgtctatgca
     3601 ccctctgtat cagagttggg tcatcgccgc tgtgcagggg aacaatgaag tgtccatgtg
     3661 ggacatggag accggagatc ggagattcac actgtgggct agttcagccc cacccctgtc
     3721 tgagctgcag cctagtccac attcagtgca cgggatctac tgttcccctg ctgatggaaa
     3781 tccaatcctg ctgaccgccg gctctgacat gaaaattagg ttttgggatc tggcttaccc
     3841 agagcgcagt tatgtggtgg ccggctcaac cagctccccc tcagtgagct actatcggaa
     3901 gatcattgag ggcacagaag tggtgcagga aatccagaac aagcagaaag tgggaccaag
     3961 tgacgatact ccaaggaggg gacctgagtc actgccagtg ggacaccatg acatcattac
     4021 cgatgtggct acattccaga ctacccaggg cttcatcgtg accgcctcca gagacggcat
     4081 cgtgaaagtg tggaagggta ccgaaaacct gtacttccaa tccggtatgg cggcctggtc
     4141 acacccgcag ttcgagaaag gtggaggtgc ccgaggtgga tcgggaggtg gatcgtggag
     4201 ccacccgcag ttcgaaaaag gattcgatta caaggatgac gatgacaagt agctcgagga
     4261 attcactcct caggtgcagg ctgcc
//
