LOCUS       sequence-458046-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli Expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  TITLE     Hurley Lab Plasmids 2025
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET/His-GST-Rab1a(Q70L)
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..693
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     651..673
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             703..723
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgaaatctt ctcaccatca ccatcaccat ggttcttcta tgtcccctat actaggttat
       61 tggaaaatta agggccttgt gcaacccact cgacttcttt tggaatatct tgaagaaaaa
      121 tatgaagagc atttgtatga gcgcgatgaa ggtgataaat ggcgaaacaa aaagtttgaa
      181 ttgggtttgg agtttcccaa tcttccttat tatattgatg gtgatgttaa attaacacag
      241 tctatggcca tcatacgtta tatagctgac aagcacaaca tgttgggtgg ttgtccaaaa
      301 gagcgtgcag agatttcaat gcttgaagga gcggttttgg atattagata cggtgtttcg
      361 agaattgcat atagtaaaga ctttgaaact ctcaaagttg attttcttag caagctacct
      421 gaaatgctga aaatgttcga agatcgttta tgtcataaaa catatttaaa tggtgatcat
      481 gtaacccatc ctgacttcat gttgtatgac gctcttgatg ttgttttata catggaccca
      541 atgtgcctgg atgcgttccc aaaattagtt tgttttaaaa aacgtattga agctatccca
      601 caaattgata agtacttgaa atccagcaag tatatagcat ggcctttgca gggctggcaa
      661 gccacgtttg gtggtggcga ccatcctcca aaagggatcg aggaaaacct gtacttccaa
      721 tccaatgcaa tgtcaagtat gaaccctgag tatgattacc tgttcaaact gctgctgatt
      781 ggcgattctg gcgtgggcaa gagctgtctg ctgctgaggt tcgccgacga tacctacaca
      841 gagtcttata tcagcaccat cggcgtggac tttaagatcc gcaccatcga gctggatggc
      901 aagacaatca agctgcagat ctgggacaca gccggccagg agcggttcag aaccatcaca
      961 agctcctact atcggggcgc ccacggcatc atcgtggtgt acgacgtgac cgatcaggag
     1021 agctttaaca atgtgaagca gtggctgcag gagatcgaca ggtatgcctc cgagaacgtg
     1081 aataagctgc tggtgggcaa caagtgcgac ctgaccacaa agaaggtggt ggattacacc
     1141 acagccaagg agttcgccga ttccctgggc atccccttcc tggagacaag cgccaagaac
     1201 gccacaaatg tggagcagtc tttcatgaca atggccgccg agatcaagaa gcggatgggc
     1261 cctggcgcca cagccggcgg cgccgagaaa agcaatgtca aaatccagtc tacacccgtc
     1321 aagcagtcag ggggaggctg ttgttaataa
//
