LOCUS       sequence-458069-       12894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector, expresses 
            Strep-KDEL-IRES-SBP-(Rat)LAMP1-mScarleti in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12894)
  AUTHORS   Li CH, Kersten N, Ozkan N, Nguyen DTM, Koppers M, Post H, Altelaar 
            M, Farias GG
  TITLE     Spatiotemporal proteomics reveals the biosynthetic lysosomal 
            membrane protein interactome in neurons.
  JOURNAL   Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w.
REFERENCE   2  (bases 1 to 12894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12"; volume: "15: 10829. doi"; pages: " 
            10.1038/s41467-024-55052-w"
COMMENT     Sequence Label: FUGW-RUSH-LAMP1-mScarleti
FEATURES             Location/Qualifiers
     source          1..12894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..1239
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1076..1095
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1268..1277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1966..2195
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2178..2197
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    2267..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2912..3022
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             4256..4948
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELY"
     primer_bind     complement(4389..4407)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4657..4676
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4992..5580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5045..5065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5463..5474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5583..5599)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5584..5600)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6105..6285
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6311..6328)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6317..6541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6587..7015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6674..6693)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6884..6905
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7024..7044)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7029..7358
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7209..7344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7271..7290
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7406..7453
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7472..7846
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7976..8097
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8013..8032)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8067..8086
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8146..8162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8146..8162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8159..8181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8170..8186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8194..8200,8201..8218,8219..8224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8239..8260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8377..8394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8548..9136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8628..8647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9307..10098,10099..10167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9930..9949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10168..10272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10347..10366)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10538..10917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10919..11117
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11072..11092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11135..11315
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11362..11487
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11980..12213
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12398..12442
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12740..12857
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
       61 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      121 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      181 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      241 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      301 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      361 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      421 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      481 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      541 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      601 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      661 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
      721 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
      781 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
      841 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
      901 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
      961 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1021 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1081 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1141 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1201 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga
     1261 ctctagagcc accatggatg tatgcgtccg tcttgccctg tggctcctct ggggactcct
     1321 cctgcaccag ggccagagcc tcagccatag tcacagtgag aaggcgacag gaaccagctc
     1381 gggcgccggt ttaaacgacc ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc
     1441 tggcattact ggaacatggt acaatcagct cgggagcacc tttattgtga ctgctggagc
     1501 cgatggagcc ctcaccggaa catacgaatc tgctgtggga aacgccgaat cacggtacgt
     1561 cctcactggc cgatacgata gtgcccctgc caccgacgga tctgggactg ccctgggatg
     1621 gactgtcgct tggaaaaaca actaccggaa tgctcattct gccacaacat ggagtggaca
     1681 gtacgtggga ggcgctgagg ctagaatcaa tacacagtgg ctgctcacat ctggcacaac
     1741 cgaggcaaat gcttggaaat ccaccctggt gggacatgac acattcacca aagtgaaacc
     1801 ctccgccgct tcaatcgatg ccgccaaaaa agccggagtc aacaacggca atcctctgga
     1861 tgccgtccag cagctcggac cgaaagatga actgtgagcg gccgcataga taactgatcc
     1921 agtgtgctgg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     1981 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     2041 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     2101 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     2161 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     2221 ggtcgagcat gcatctaggg cggccaattc cgcccctctc cccccccccc tctccctccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa gaatggcggc cccgggcgcc cggcggccgc tgctcctgtt
     2881 gctgctggca ggccttgcac acagcaccgg tgacgagaag accactggtt ggcgaggtgg
     2941 acacgttgtt gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca
     3001 cccacaaggt caacgtgaac caggatccgc tggctccgct gcaggttctg gcgctggatc
     3061 tgctgctggt tctggcgaat tcgccccagc actgttcgag gtgaaagaca acaacggcac
     3121 agcgtgtata atggccagct tctctgcctc ctttctgacc acctatgatg ctggacatgt
     3181 ttctaaggtc tcgaatatga ccctgccagc ctctgcagaa gtcctgaaga atagcagctc
     3241 ttgtggtgaa aagaatgctt ctgagcccac cctcgcaatc acctttggag aaggatattt
     3301 actgaaactc accttcacaa aaaacacaac acgttacagt gtccagcaca tgtatttcac
     3361 atataacctg tcagacacac aattctttcc caatgccagc tccaaagggc ccgacactgt
     3421 ggattccaca actgacatca aggcagacat caacaaaaca taccgatgtg tcagcgacat
     3481 cagggtctac atgaagaatg tgaccattgt gctctgggac gctactatcc aggcctacct
     3541 gccgagtagc aacttcagca aggaagagac acgctgccca caggatcaac cttccccaac
     3601 tactgggcca cccagcccct caccaccact tgtgcccaca aaccccagtg tgtccaagta
     3661 caatgtgact ggtgacaatg gaacctgcct gctggcctct atggcactgc aactcaacat
     3721 cacctacatg aagaaggaca acacgactgt gaccagagca ttcaacatca acccaagtga
     3781 caaatatagt gggacttgcg gtgcccagtt ggtgaccctg aaggtgggga acaagagcag
     3841 agtcctggag ctgcagtttg ggatgaatgc cacttctagc ctgtttttcc tgcaaggagt
     3901 tcagttgaac atgactcttc ctgatgccat agagcccacg ttcagcacct ccaactattc
     3961 cctgaaagct cttcaggcca gtgtcggcaa ctcatacaag tgcaacagtg aggagcacat
     4021 ctttgtcagc aaggcgctcg ccctcaatgt cttcagcgtg caagtccagg ctttcagggt
     4081 agaaagtgac aggtttgggt ctgtggaaga gtgtgtacag gacggtaaca acatgctgat
     4141 ccccattgct gtgggcgggg ccctggcagg gctggtcctc atcgtcctca tcgcctacct
     4201 catcggcagg aagaggagtc acgcgggcta tcagaccatc ggtagtggta gtggtatggt
     4261 gagcaagggc gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc
     4321 catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     4381 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct
     4441 gtcccctcag ttcatgtacg gctccagggc cttcatcaag caccccgccg acatccccga
     4501 ctactataag cagtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     4561 cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa
     4621 ggtgaagctc cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat
     4681 gggctgggaa gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat
     4741 taagatggcc ctgcgcctga aggacggcgg ccgctacctg gcggacttca agaccaccta
     4801 caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca agttggacat
     4861 cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca
     4921 ctccaccggc ggcatggacg agctgtacta agaattcgat ccgttaacaa ttcgatatca
     4981 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5041 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5101 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5161 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5221 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5281 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5341 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5401 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5461 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5521 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5581 atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag
     5641 cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc
     5701 cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     5821 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     5881 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     5941 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     6001 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     6061 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     6121 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6181 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6241 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     6301 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6361 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6421 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6481 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6541 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6601 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6661 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6721 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6781 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6841 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6901 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6961 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7021 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7081 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7141 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7201 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7261 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7321 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7381 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     7441 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     7501 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7561 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     7621 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     7681 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     7741 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     7801 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     7861 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     7921 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     7981 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8041 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8101 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8161 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8221 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8281 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8341 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8401 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8461 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8521 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8581 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8641 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8701 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8761 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8821 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8881 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8941 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9001 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9061 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9121 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9181 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9241 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9301 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9361 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9421 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9481 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9541 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9601 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9661 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9721 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9781 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9841 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9901 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9961 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10021 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10081 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10141 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10201 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10261 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
    10321 cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    10381 tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct
    10441 acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt
    10501 gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat
    10561 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    10621 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    10681 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    10741 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    10801 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    10861 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
    10921 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
    10981 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
    11041 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
    11101 gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg
    11161 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
    11221 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
    11281 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
    11341 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
    11401 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
    11461 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
    11521 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
    11581 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
    11641 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
    11701 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
    11761 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
    11821 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
    11881 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
    11941 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
    12001 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
    12061 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
    12121 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
    12181 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
    12241 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    12301 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    12361 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    12421 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    12481 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    12541 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    12601 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
    12661 agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa
    12721 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    12781 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    12841 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggt
//
