LOCUS       sequence-458071-       13016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector, expresses Strep-KDEL-IRES-SBP-(Rat)LAMP1-V5-APEX2
            in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13016)
  AUTHORS   Li CH, Kersten N, Ozkan N, Nguyen DTM, Koppers M, Post H, Altelaar 
            M, Farias GG
  TITLE     Spatiotemporal proteomics reveals the biosynthetic lysosomal 
            membrane protein interactome in neurons.
  JOURNAL   Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w.
REFERENCE   2  (bases 1 to 13016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12"; volume: "15: 10829. doi"; pages: " 
            10.1038/s41467-024-55052-w"
COMMENT     Sequence Label: FUGW-RUSH-LAMP1-V5-APEX2
FEATURES             Location/Qualifiers
     source          1..13016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..1239
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1076..1095
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1268..1277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1966..2195
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2178..2197
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    2267..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2912..3022
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             4277..4318
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4325..5071
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     misc_feature    5114..5702
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5167..5187)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5585..5596)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5705..5721)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5706..5722)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6227..6407
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6433..6450)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6439..6663
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6709..7137
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6796..6815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7006..7027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7146..7166)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7151..7480
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7331..7466
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7393..7412
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7528..7575
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7594..7968
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8098..8219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8135..8154)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8189..8208
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8268..8284)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8268..8284)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8281..8303)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8292..8308
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8316..8322,8323..8340,8341..8346))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8361..8382
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8499..8516)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8670..9258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8750..8769)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9429..10220,10221..10289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10052..10071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10290..10394)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10469..10488)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10660..11039
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11041..11239
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11194..11214
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11257..11437
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11484..11609
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12102..12335
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12520..12564
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12862..12979
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
       61 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      121 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      181 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      241 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      301 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      361 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      421 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      481 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      541 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      601 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      661 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
      721 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
      781 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
      841 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
      901 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
      961 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1021 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1081 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1141 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1201 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga
     1261 ctctagagcc accatggatg tatgcgtccg tcttgccctg tggctcctct ggggactcct
     1321 cctgcaccag ggccagagcc tcagccatag tcacagtgag aaggcgacag gaaccagctc
     1381 gggcgccggt ttaaacgacc ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc
     1441 tggcattact ggaacatggt acaatcagct cgggagcacc tttattgtga ctgctggagc
     1501 cgatggagcc ctcaccggaa catacgaatc tgctgtggga aacgccgaat cacggtacgt
     1561 cctcactggc cgatacgata gtgcccctgc caccgacgga tctgggactg ccctgggatg
     1621 gactgtcgct tggaaaaaca actaccggaa tgctcattct gccacaacat ggagtggaca
     1681 gtacgtggga ggcgctgagg ctagaatcaa tacacagtgg ctgctcacat ctggcacaac
     1741 cgaggcaaat gcttggaaat ccaccctggt gggacatgac acattcacca aagtgaaacc
     1801 ctccgccgct tcaatcgatg ccgccaaaaa agccggagtc aacaacggca atcctctgga
     1861 tgccgtccag cagctcggac cgaaagatga actgtgagcg gccgcataga taactgatcc
     1921 agtgtgctgg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     1981 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     2041 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     2101 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     2161 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     2221 ggtcgagcat gcatctaggg cggccaattc cgcccctctc cccccccccc tctccctccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa gaatggcggc cccgggcgcc cggcggccgc tgctcctgtt
     2881 gctgctggca ggccttgcac acagcaccgg tgacgagaag accactggtt ggcgaggtgg
     2941 acacgttgtt gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca
     3001 cccacaaggt caacgtgaac caggatccgc tggctccgct gcaggttctg gcgctggatc
     3061 tgctgctggt tctggcgaat tcgccccagc actgttcgag gtgaaagaca acaacggcac
     3121 agcgtgtata atggccagct tctctgcctc ctttctgacc acctatgatg ctggacatgt
     3181 ttctaaggtc tcgaatatga ccctgccagc ctctgcagaa gtcctgaaga atagcagctc
     3241 ttgtggtgaa aagaatgctt ctgagcccac cctcgcaatc acctttggag aaggatattt
     3301 actgaaactc accttcacaa aaaacacaac acgttacagt gtccagcaca tgtatttcac
     3361 atataacctg tcagacacac aattctttcc caatgccagc tccaaagggc ccgacactgt
     3421 ggattccaca actgacatca aggcagacat caacaaaaca taccgatgtg tcagcgacat
     3481 cagggtctac atgaagaatg tgaccattgt gctctgggac gctactatcc aggcctacct
     3541 gccgagtagc aacttcagca aggaagagac acgctgccca caggatcaac cttccccaac
     3601 tactgggcca cccagcccct caccaccact tgtgcccaca aaccccagtg tgtccaagta
     3661 caatgtgact ggtgacaatg gaacctgcct gctggcctct atggcactgc aactcaacat
     3721 cacctacatg aagaaggaca acacgactgt gaccagagca ttcaacatca acccaagtga
     3781 caaatatagt gggacttgcg gtgcccagtt ggtgaccctg aaggtgggga acaagagcag
     3841 agtcctggag ctgcagtttg ggatgaatgc cacttctagc ctgtttttcc tgcaaggagt
     3901 tcagttgaac atgactcttc ctgatgccat agagcccacg ttcagcacct ccaactattc
     3961 cctgaaagct cttcaggcca gtgtcggcaa ctcatacaag tgcaacagtg aggagcacat
     4021 ctttgtcagc aaggcgctcg ccctcaatgt cttcagcgtg caagtccagg ctttcagggt
     4081 agaaagtgac aggtttgggt ctgtggaaga gtgtgtacag gacggtaaca acatgctgat
     4141 ccccattgct gtgggcgggg ccctggcagg gctggtcctc atcgtcctca tcgcctacct
     4201 catcggcagg aagaggagtc acgcgggcta tcagaccatc ggatccgctg gctccgctgc
     4261 tggttctggc gaattcggta agcctatccc taaccctctc ctcggtctcg attctacggg
     4321 gcccggaaag tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa
     4381 gaagaagctc agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc
     4441 attccactct gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat
     4501 caagcaccct gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct
     4561 tttggagcca ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc
     4621 tggcgttgtt gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga
     4681 ggacaagcct gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca
     4741 tttgagagat gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc
     4801 tgggggtcac actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac
     4861 ctctaatcct cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga
     4921 aggtctcctt cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct
     4981 cgttgacaaa tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca
     5041 aaagctttcc gagcttgggt ttgctgatgc ctaaaattcg atccgttaac aattcgatat
     5101 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5161 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5221 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5281 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5341 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5401 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5461 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5521 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5581 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5641 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5701 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     5761 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     5821 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5881 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5941 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     6001 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6061 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     6121 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     6181 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     6241 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6301 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6361 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     6421 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6481 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6541 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6601 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6661 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6721 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6781 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6841 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6901 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6961 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7021 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7081 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7141 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7201 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7261 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7321 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7381 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7501 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     7561 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     7621 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     7681 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     7741 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     7801 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     7861 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     7921 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     7981 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8041 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8101 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8161 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8221 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8281 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8341 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8401 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8461 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8521 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8581 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8641 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8701 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8761 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8821 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8881 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8941 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9001 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9061 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9121 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9181 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9241 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9301 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9361 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9421 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9481 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9541 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9601 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9661 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9721 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9781 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9841 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9901 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9961 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10021 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10081 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10141 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10201 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10261 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10321 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10381 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
    10441 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    10501 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
    10561 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
    10621 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
    10681 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
    10741 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
    10801 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
    10861 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
    10921 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
    10981 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
    11041 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
    11101 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
    11161 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
    11221 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
    11281 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
    11341 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
    11401 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
    11461 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    11521 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    11581 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    11641 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    11701 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    11761 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    11821 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    11881 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    11941 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    12001 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    12061 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    12121 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    12181 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    12241 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    12301 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    12361 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    12421 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    12481 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    12541 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    12601 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    12661 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    12721 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    12781 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
    12841 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    12901 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    12961 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggt
//
