LOCUS       sequence-458072-       10796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10796)
  AUTHORS   Li CH, Kersten N, Ozkan N, Nguyen DTM, Koppers M, Post H, Altelaar 
            M, Farias GG
  TITLE     Spatiotemporal proteomics reveals the biosynthetic lysosomal 
            membrane protein interactome in neurons.
  JOURNAL   Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w.
REFERENCE   2  (bases 1 to 10796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12"; volume: "15: 10829. doi"; pages: " 
            10.1038/s41467-024-55052-w"
COMMENT     Sequence Label: FUGW-RUSH-Strep-KDEL
FEATURES             Location/Qualifiers
     source          1..10796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..1239
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1076..1095
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1268..1277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1966..2195
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2178..2197
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    2267..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    2894..3482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2947..2967)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3365..3376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3485..3501)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3486..3502)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4007..4187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4213..4230)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4219..4443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4489..4917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4576..4595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4786..4807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4926..4946)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4931..5260
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5111..5246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5173..5192
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5308..5355
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5374..5748
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    5878..5999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5915..5934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5969..5988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6048..6064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6048..6064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6061..6083)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6072..6088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6096..6102,6103..6120,6121..6126))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6141..6162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6279..6296)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6450..7038)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6530..6549)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7209..8000,8001..8069))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7832..7851
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8070..8174)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8249..8268)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8440..8819
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8821..9019
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8974..8994
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9037..9217
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9264..9389
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9882..10115
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10300..10344
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10642..10759
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
       61 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      121 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      181 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      241 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      301 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      361 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      421 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      481 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      541 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      601 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      661 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
      721 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
      781 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
      841 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
      901 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
      961 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1021 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1081 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1141 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1201 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga
     1261 ctctagagcc accatggatg tatgcgtccg tcttgccctg tggctcctct ggggactcct
     1321 cctgcaccag ggccagagcc tcagccatag tcacagtgag aaggcgacag gaaccagctc
     1381 gggcgccggt ttaaacgacc ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc
     1441 tggcattact ggaacatggt acaatcagct cgggagcacc tttattgtga ctgctggagc
     1501 cgatggagcc ctcaccggaa catacgaatc tgctgtggga aacgccgaat cacggtacgt
     1561 cctcactggc cgatacgata gtgcccctgc caccgacgga tctgggactg ccctgggatg
     1621 gactgtcgct tggaaaaaca actaccggaa tgctcattct gccacaacat ggagtggaca
     1681 gtacgtggga ggcgctgagg ctagaatcaa tacacagtgg ctgctcacat ctggcacaac
     1741 cgaggcaaat gcttggaaat ccaccctggt gggacatgac acattcacca aagtgaaacc
     1801 ctccgccgct tcaatcgatg ccgccaaaaa agccggagtc aacaacggca atcctctgga
     1861 tgccgtccag cagctcggac cgaaagatga actgtgagcg gccgcataga taactgatcc
     1921 agtgtgctgg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     1981 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     2041 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     2101 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     2161 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     2221 ggtcgagcat gcatctaggg cggccaattc cgcccctctc cccccccccc tctccctccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa gaattcttcg aagctagcgg atccgttaac aattcgatat
     2881 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2941 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3001 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3061 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3121 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3181 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3241 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3301 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3361 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     3421 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     3481 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     3541 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     3601 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     3661 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     3721 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     3781 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     3841 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     3901 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     3961 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     4021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     4081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     4141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     4201 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4261 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4321 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4381 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4441 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     4501 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4561 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4621 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4681 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     4741 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4801 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     4861 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     4921 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     4981 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5161 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5221 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5281 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     5341 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     5401 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     5461 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     5521 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     5581 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     5641 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     5701 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     5761 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5821 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5881 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5941 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6001 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6061 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6121 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6181 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6241 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6361 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6421 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6481 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6541 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6601 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6661 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6721 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6781 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6841 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6901 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6961 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7021 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7081 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7141 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7201 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7261 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7321 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7381 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7441 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7501 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7561 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7621 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7681 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7741 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7801 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7861 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7921 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7981 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8041 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8101 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8161 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8221 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8281 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8341 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8401 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
     8461 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8521 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8581 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8641 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     8701 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     8761 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     8821 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     8881 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     8941 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     9001 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     9061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9181 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9241 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9301 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9361 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9421 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     9481 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9541 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9601 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9661 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     9721 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9781 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9841 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9901 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9961 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    10021 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    10081 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    10141 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    10201 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    10561 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
    10621 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10681 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10741 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggt
//
