LOCUS       sequence-458073-       12942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector, expresses 
            Strep-KDEL-IRES-SBP-(Rat)LAMP2A-mNeonGreen in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12942)
  AUTHORS   Li CH, Kersten N, Ozkan N, Nguyen DTM, Koppers M, Post H, Altelaar 
            M, Farias GG
  TITLE     Spatiotemporal proteomics reveals the biosynthetic lysosomal 
            membrane protein interactome in neurons.
  JOURNAL   Nat Commun. 2024 Dec;15: 10829. doi: 10.1038/s41467-024-55052-w.
REFERENCE   2  (bases 1 to 12942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-12"; volume: "15: 10829. doi"; pages: " 
            10.1038/s41467-024-55052-w"
COMMENT     Sequence Label: FUGW-RUSH-LAMP2A-mNeonGreen
FEATURES             Location/Qualifiers
     source          1..12942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..1239
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1076..1095
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      1268..1277
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          1966..2195
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     2178..2197
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    2267..2840
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2434..2451)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2661..2680
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2921..3031
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="DEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             4289..4999
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4544..4553
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5040..5628
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5093..5113)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5511..5522)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5631..5647)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5632..5648)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6153..6333
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6359..6376)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6365..6589
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6635..7063
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6722..6741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6932..6953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7072..7092)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7077..7406
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7257..7392
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7319..7338
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7454..7501
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7520..7894
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8024..8145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8061..8080)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8115..8134
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8194..8210)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8194..8210)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8207..8229)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8218..8234
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8242..8248,8249..8266,8267..8272))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8287..8308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8425..8442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8596..9184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8676..8695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9355..10146,10147..10215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9978..9997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10216..10320)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10395..10414)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10586..10965
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10967..11165
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11120..11140
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             11183..11363
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11410..11535
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12028..12261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12446..12490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12788..12905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg cctcccgcgg
       61 gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc gagcgtcctg
      121 atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg gccttagaac
      181 cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg gcactggttt
      241 tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat tctgcggagg
      301 gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt gtggcacagc
      361 tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc
      421 acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg ggccgctcgg
      481 tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt
      541 gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg agtcttgaat
      601 ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg catggtgggc
      661 ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat tcgggtgaga
      721 tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg gagaactcgg
      781 tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg cacccgtacc
      841 tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca
      901 gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt
      961 tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg aggggaggga
     1021 taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa gtagctgaag
     1081 ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca
     1141 ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac tagtaaattg
     1201 tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc tgcaggtcga
     1261 ctctagagcc accatggatg tatgcgtccg tcttgccctg tggctcctct ggggactcct
     1321 cctgcaccag ggccagagcc tcagccatag tcacagtgag aaggcgacag gaaccagctc
     1381 gggcgccggt ttaaacgacc ctagcaaaga ctcaaaagct caggtgtccg ctgccgaggc
     1441 tggcattact ggaacatggt acaatcagct cgggagcacc tttattgtga ctgctggagc
     1501 cgatggagcc ctcaccggaa catacgaatc tgctgtggga aacgccgaat cacggtacgt
     1561 cctcactggc cgatacgata gtgcccctgc caccgacgga tctgggactg ccctgggatg
     1621 gactgtcgct tggaaaaaca actaccggaa tgctcattct gccacaacat ggagtggaca
     1681 gtacgtggga ggcgctgagg ctagaatcaa tacacagtgg ctgctcacat ctggcacaac
     1741 cgaggcaaat gcttggaaat ccaccctggt gggacatgac acattcacca aagtgaaacc
     1801 ctccgccgct tcaatcgatg ccgccaaaaa agccggagtc aacaacggca atcctctgga
     1861 tgccgtccag cagctcggac cgaaagatga actgtgagcg gccgcataga taactgatcc
     1921 agtgtgctgg aattaattcg ctgtctgcga gggccagctg ttggggtgag tactccctct
     1981 caaaagcggg catgacttct gcgctaagat tgtcagtttc caaaaacgag gaggatttga
     2041 tattcacctg gcccgcggtg atgcctttga gggtggccgc gtccatctgg tcagaaaaga
     2101 caatcttttt gttgtcaagc ttgaggtgtg gcaggcttga gatctggcca tacacttgag
     2161 tgacaatgac atccactttg cctttctctc cacaggtgtc cactcccagg tccaactgca
     2221 ggtcgagcat gcatctaggg cggccaattc cgcccctctc cccccccccc tctccctccc
     2281 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     2341 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     2401 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     2461 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     2521 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     2581 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     2641 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     2701 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     2761 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2821 tttgaaaaac acgatgataa gaatgcgcct cctctctccg gttacgggct cgaagctcgt
     2881 cctgctcttt ctgttcctgg gagcagttcg gtccaccggt gacgagaaga ccactggttg
     2941 gcgaggtgga cacgttgttg aaggactggc tggggaactt gaacaacttc gtgcacgact
     3001 ggagcatcac ccacaaggtc aacgtgaacc aggatccgct ggctccgctg caggttctgg
     3061 cgctggatct gctgctggtt ctggcgaatt cgatgcattg aaacttaatt tgacagattc
     3121 aaagggtact tgcctttatg cagaatggga gatgaatttc acaataacat atgaagctct
     3181 aaaagtcaat gaaactgtaa ccattacagt gcctgacaag gtgacataca atggaagcag
     3241 ttgtggcgat gataagaatg gtgccaaaat aatgatacaa tatggatcca ctctctcttg
     3301 ggctgtgaat ttcaccaagg aagcatctca gtattttatt aacaacatca cgctttctta
     3361 caacactaat gataccaaaa catttcctgg tgctgtacct aaaggaatcc ttactgttat
     3421 cattcctgtg ggatcccagc ttccattggg tgtcatcttt aagtgcagta gtgttttaac
     3481 tttcaacctg agtcctgttg ttcagcacta ttggggcatt cacctgcaag cttttgtcca
     3541 aaatggtaca gtgagtaaac atgaacaagt gtgtaaggag gacaaaactg ctaccactgt
     3601 agcacccatc attcacacca ccgtgccatc gcctactacg acactcactc caacttcaat
     3661 acccgttcca actccaacgg ttggaaacta caccatttct aatggcaatg ctacctgtct
     3721 gctggctacc atggggctgc agctgaacat caccgaggag aaggtgcctt tcatttttaa
     3781 catcaaccct gccataacca acttcaccgg cagctgtcag ccccaaacag ctcaacttag
     3841 gctgaacaac agccaaatta agtatctcga ctttatcttt gctgtgaaaa atgaaaaacg
     3901 gttctatctg aaggaagtga atgtcaacat gtatttggct aatggctcag ctttccatgt
     3961 ttcaaataac aaccttagct tctgggatgc tcctctggga agttcttata tgtgcaacaa
     4021 agagcaggtg gtttccgtgt ctcgaacatt tcagataaat acctttaacc tgaaggtgca
     4081 accttttaat gtgacgaaag gagagtattc tacagctcaa gactgcagtg cagatgaaga
     4141 caacttcctt gtgcccatag cggtgggagc agccctggga ggagtactta ttctagtgtt
     4201 gctggcttat tttattggtc tcaagcgcca tcatactgga tatgagcaat ttggagcttc
     4261 cggctccgct ggttccggag cttctggcat ggtgagcaag ggcgaggagg ataacatggc
     4321 ctctctccca gcgacacatg agttacacat ctttggctcc atcaacggtg tggactttga
     4381 catggtgggt cagggcaccg gcaatccaaa tgatggttat gaggagttaa acctgaagtc
     4441 caccaagggt gacctccagt tctccccctg gattctggtc cctcatatcg ggtatggctt
     4501 ccatcagtac ctgccctacc ctgacgggat gtcgcctttc caggccgcca tggtagatgg
     4561 ctccggatac caagtccatc gcacaatgca gtttgaagat ggtgcctccc ttactgttaa
     4621 ctaccgctac acctacgagg gaagccacat caaaggagag gcccaggtga aggggactgg
     4681 tttccctgct gacggtcctg tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc
     4741 gaagaagact taccccaacg acaaaaccat catcagtacc tttaagtgga gttacaccac
     4801 tggaaatggc aagcgctacc ggagcactgc gcggaccacc tacacctttg ccaagccaat
     4861 ggcggctaac tatctgaaga accagccgat gtacgtgttc cgtaagacgg agctcaagca
     4921 ctccaagacc gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat
     4981 ggacgagctg tacaagtaag aattcgatcc gttaacaatt cgatatcaag cttatcgata
     5041 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5101 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5161 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5221 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5281 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5341 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5401 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     5461 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5521 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5581 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     5641 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     5701 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5761 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5821 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     5881 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     5941 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     6001 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     6061 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     6121 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     6181 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6241 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6301 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     6361 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     6421 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6481 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6541 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6601 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7081 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7141 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7201 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7261 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7321 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7381 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     7441 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     7501 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     7561 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     7621 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     7681 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     7741 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     7801 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     7861 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     7921 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     7981 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8041 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8101 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8161 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8221 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8281 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8341 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8401 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8461 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8521 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8581 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8641 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8701 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8761 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8821 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8881 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8941 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9001 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9061 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9121 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9181 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9241 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9301 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9361 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9421 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9481 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9541 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9601 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9661 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9721 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9781 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9841 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9901 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9961 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10021 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10081 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10141 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10201 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10261 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10321 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    10381 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    10441 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    10501 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
    10561 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
    10621 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    10681 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    10741 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10801 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10861 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10921 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
    10981 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
    11041 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
    11101 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
    11161 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    11221 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    11281 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    11341 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    11401 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    11461 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    11521 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    11581 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    11641 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    11701 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    11761 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    11821 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
    11881 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
    11941 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
    12001 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
    12061 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
    12121 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
    12181 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
    12241 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
    12301 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
    12361 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
    12421 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
    12481 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
    12541 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
    12601 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
    12661 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
    12721 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
    12781 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    12841 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    12901 attttcgggt ttattacagg gacagcagag atccagtttg gt
//
