LOCUS       sequence-458092-       11470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNA targeting SlPDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11470)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 11470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pEE485
FEATURES             Location/Qualifiers
     source          1..11470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    22..46
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        575..920
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     863..883
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2475..2550
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3548..3565
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3600..3852
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3874..3898
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5199..5828
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6257..7330
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7396..7590
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7826..7848)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7934..8074
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7989..8008
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8089..8106)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8260..8848)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8340..8359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10273..11067)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
       61 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcc
      121 taggccacca tgttgggccc ggcgcgccaa gcttgcatgc ctgcaggtca acatggtgga
      181 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc
      241 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      301 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      361 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
      421 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      481 agtggattga tgtgatggtc aacatggtgg agcacgacac tctcgtctac tccaagaata
      541 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      601 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      661 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
      721 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
      781 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
      841 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
      901 catttcattt ggagaggata aaacattgca cctatggtgt tgccctggct ggggtatgtc
      961 agtgatcgca gtagaatgta ctaattgaca agttggagaa tacggtagaa cgtccttatc
     1021 caacacagcc tttatccctc tccctgacga ggtttttgtc agtgtaatat ttctttttga
     1081 actatccagc ttagtaccgt acgggaaagt gactggtgtg cttatctttg aaatgttact
     1141 ttgggtttcg gttctttagg ttagtaagaa agcacttgtc ttctcataca aaggaaaacc
     1201 tgagacgtat cgcttacgaa agtagcaatg aaagaaaggt ggtggtttta atcgctaccg
     1261 caaaaacgat ggggtcgttt taattaactt ctcctacgca agcgtctaaa cggacgttgg
     1321 ggttttgcta gtttctttag agaaaactag ctaagtcttt aatgttatca ttagagatgg
     1381 cataaatata atacttgtgt ctgctgataa gatcatttta atttggacga ttagacttgt
     1441 tgaactacag gttactgaat cacttgcgct aatcaacatg ggagatatgt acgatgaatc
     1501 atttgacaag tcgggcggtc ctgctgactt gatggacgat tcttgggtgg aatcagtttc
     1561 gtggaaagat ctgttgaaga agttacacag cataaaattt gcactacagt ctggtagaga
     1621 tgagatcact gggttactag cggcactgaa tagacagtgt ccttattcac catatgagca
     1681 gtttccagat aagaaggtgt atttcctttt agactcacgg gctaacagtg ctcttggtgt
     1741 gattcagaac gcttcagcgt tcaagagacg agctgatgag aagaatgcag tggcgggtgt
     1801 tacaaatatt cctgcgaatc caaacacaac ggttacgacg aaccaaggga gtactactac
     1861 taccaaggcg aacactggct cgactttgga agaagacttg tacacttatt acaaattcga
     1921 tgatgcctct acagctttcc acaaatctct aacttcgtta gagaacatgg agttgaagag
     1981 ttattaccga aggaactttg agaaagtatt cgggattaag tttggtggag cagctgctag
     2041 ttcatctgca ccgcctccag cgagtggagg tccgatacgt cctaatccct agggatttaa
     2101 ggacgtgaac tctgttgaga tctctgtgaa attcagaggg tgggtgatac catattcact
     2161 gatgccatta gcgacatcta aatagggcta attgtgacta atttgaggga atttccttta
     2221 ccattgacgt cagtgtcgtt ggtagcattt gagtttcgga gcatcttgtt ctggggtttc
     2281 acactatctt tagagaaagt gttaagttaa ttaagttatc ttaattaaga gcataattat
     2341 actgatttgt ctctcgttga tagagtctat cattctgtta ctaaaaattt gacaactcgg
     2401 tttgctgacc tactggttac tgtatcactt acccgagtta acgagtctag aaacggactc
     2461 ttgccagcaa tgctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2521 caacttgaaa aagtggcacc gagtcggtgc tccctagtct ataaatataa gagaccctct
     2581 tatagtaagc agagttgttg gagacgttct tgatccgttt aatagatcaa tcactctaaa
     2641 ggttacttat ggccaaagag aggtgactaa tggcttggat ctaaggcctt ctcaggttca
     2701 aaacaagcca agagttgaga ttggtggaga agacctcagg aacttctata ctttggttat
     2761 ggtggatcca gatgttccaa gtcctagcaa ccctcacctc cgagaatatc tccattggtt
     2821 ggtgactgat atccctgcta caactggaac aacctttggc aatgagattg tgtgttacga
     2881 aaatccaagt cccactgcag gaattcatcg tgtcgtgttt atattgtttc gacagcttgg
     2941 caggcaaaca gtgtatgcac cagggtggcg ccagaacttc aacactcgcg agtttgctga
     3001 gatctacaat ctcggccttc ccgtggccgc agttttctac aattgtcaga gggagagtgg
     3061 ctgcggagga agaagacttt agagtgccgg gcatgtcccg aagacattaa actacggttc
     3121 tttaagtaga tccgtgtctg aagttttagg ttcaatttaa acctacgaga ttgacattct
     3181 cgactgatct tgattgatcg gtaagtcttt tgtaatttaa ttttcttttt gattttattt
     3241 taaattgtta tctgtttctg tgtatagact gtttgagatc ggcgtttggc cgactcattg
     3301 tcttaccata ggggaacgga ctttgtttgt gttgttattt tatttgtatt ttattaaaat
     3361 tctcaacgat ctgaaaaagc ctcgcggcta agagattgtt ggggggtgag taagtacttt
     3421 taaagtgatg atggttacaa aggcaaaagg ggtaaaaccc ctcgcctacg taagcgttat
     3481 tacgcccgtc tgtacttata tcagtacact gacgagtccc taaaggacga aacgggagaa
     3541 cgctagccac caccaccacc accacgtgtg aattacaggt gaccagctcg aatttccccg
     3601 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     3661 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     3721 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     3781 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     3841 tgttactaga tcgggaatta aactatcagt gtttgacagg atatattggc gggtaaacct
     3901 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     3961 cgttcgtcca tttgtatgtg catgccaacc acagggttcc cctcgggatc aaagtacttt
     4021 gatccaaccc ctccgctgct atagtgcagt cggcttctga cgttcagtgc agccgtcttc
     4081 tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag gctgccgccc tgcccttttc
     4141 ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag aatacttgcg actagaaccg
     4201 gagacattac gccatgaaca agagcgccgc cgctggcctg ctgggctatg cccgcgtcag
     4261 caccgacgac caggacttga ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa
     4321 gctgttttcc gagaagatca ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct
     4381 tgaccaccta cgccctggcg acgttgtgac agtgaccagg ctagaccgcc tggcccgcag
     4441 cacccgcgac ctactggaca ttgccgagcg catccaggag gccggcgcgg gcctgcgtag
     4501 cctggcagag ccgtgggccg acaccaccac gccggccggc cgcatggtgt tgaccgtgtt
     4561 cgccggcatt gccgagttcg agcgttccct aatcatcgac cgcacccgga gcgggcgcga
     4621 ggccgccaag gcccgaggcg tgaagtttgg cccccgccct accctcaccc cggcacagat
     4681 cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc gtgaaagagg cggctgcact
     4741 gcttggcgtg catcgctcga ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc
     4801 caccgaggcc aggcggcgcg gtgccttccg tgaggacgca ttgaccgagg ccgacgccct
     4861 ggcggccgcc gagaatgaac gccaagagga acaagcatga aaccgcacca ggacggccag
     4921 gacgaaccgt ttttcattac cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg
     4981 ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct
     5041 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     5101 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     5161 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     5221 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     5281 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     5341 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     5401 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     5461 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     5521 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     5581 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     5641 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     5701 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     5761 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     5821 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
     5881 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
     5941 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
     6001 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
     6061 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
     6121 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
     6181 gttggccagg cgtaagcggc tgggttgcct gccggccctg caatggcact ggaaccccca
     6241 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
     6301 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
     6361 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga
     6421 atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca agggcgacga
     6481 gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata gtcgcagcat
     6541 catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg
     6601 ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca tggccagtgt
     6661 gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca tgaaccgata
     6721 ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg cggacgtact
     6781 caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag aaacctgcat
     6841 tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga acggccgcct
     6901 ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa agagcgaaac
     6961 cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg agatcacaga
     7021 aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg atcccggcat
     7081 cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag ccagatggtt
     7141 gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt tctgtttcac
     7201 cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg aggaggcggg
     7261 gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc
     7321 cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg
     7381 aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt acattgggaa
     7441 ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac acatgtaagt
     7501 gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa aacttattaa
     7561 aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag ccgaagagct
     7621 gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc
     7681 tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc taccagggcg
     7741 cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca ccctgcctcg
     7801 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     7861 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7921 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     7981 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8041 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     8101 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8161 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8221 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8281 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8341 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8401 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8461 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8521 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8581 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8641 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8701 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8761 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8821 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8881 cgctcagtgg aacgaaaact cacgttaagg gctgatgaat cccctaatga tttttatcaa
     8941 aatcattaag ttaaggtaga tacacatctt gtcatatgat caaatggttt cgccaaaaat
     9001 caataatcag acaacaaaat gtgcgaactc gatattttac acgactctct ttaccaattc
     9061 tgccccgaat tacacttaaa acgactcaac agcttaacgt tggcttgcca cgccttactt
     9121 gactgtaaaa ctctcactct taccgaactt ggccgtaacc tgccaaccaa agcgagaaca
     9181 aaacataaca tcaaacgaat cgaccgattg ttaggtaatc gtcacctcca caaagagcga
     9241 ctcgctgtat accgttggca tgctagcttt atctgttcgg gcaatacgat gcccattgta
     9301 cttgttgact ggtctgatat ccgtgagcaa aaacggctta tggtattgcg agcttcagtc
     9361 gcactacacg gtcgttctgt tactctttat gagaaagcgt tcccgctttc agagcaatgt
     9421 tcaaagaaag ctcatgacca atttctagcc gaccttgcga gcattctacc gagtaacacc
     9481 acaccgctca ttgtcagtga tgctggcttt aaagtgccat ggtataaatc cgttgagaag
     9541 ctgggttggt actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg
     9601 gaaaactgga aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta
     9661 ggctataaga ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct
     9721 cgctctaaag gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct
     9781 aaaatctact cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa
     9841 attcgaacac ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc
     9901 ttccgagact tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc
     9961 tcagagcgtt ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt
    10021 gcgggcgttc atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga
    10081 aatcgaaacg tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac
    10141 acaataacaa gggaagactc actcgtggct gcaaccctgc ttactcaaaa tctattcaca
    10201 catggttacg ttttggggaa attatgaggg gatctctcag cgttaaggga ttttggtcat
    10261 gcattctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    10321 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    10381 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    10441 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    10501 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    10561 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    10621 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    10681 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    10741 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    10801 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    10861 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    10921 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    10981 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    11041 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    11101 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    11161 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    11221 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
    11281 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
    11341 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
    11401 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
    11461 gctgccggtc
//
