LOCUS       sequence-458094-       11798 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbPDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11798)
  AUTHORS   Liu D, Myers EA, Xuan S, Prichard LE, Donahue LI, Ellison EE, 
            Starker CG, Voytas DF
  TITLE     Heritable, multinucleotide deletions in plants using viral delivery 
            of a repair exonuclease and guide RNAs.
  JOURNAL   Plant Physiol. 2024 Mar 29;194(4):2229-2239. doi: 
            10.1093/plphys/kiae015.
  PUBMED    38243587
REFERENCE   2  (bases 1 to 11798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Plant Physiol"; date: "2024-03-29- 29"; volume: "194"; issue: "4"; 
            pages: "2229-2239"
COMMENT     Sequence Label: VirTREX2-HLDel-1_NbPDS
FEATURES             Location/Qualifiers
     source          1..11798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             460..477
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      512..764
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    786..810
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2111..2740
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3169..4242
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4308..4502
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4738..4760)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4846..4986
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4901..4920
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5001..5018)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5172..5759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5252..5271)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7184..7978)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8403..8427
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8956..9301
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9244..9264
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             10872..10892
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgggcatgt cccgaagaca ttaaactacg gttctttaag tagatccgtg tctgaagttt
       61 taggttcaat ttaaacctac gagattgaca ttctcgactg atcttgattg atcggtaagt
      121 cttttgtaat ttaattttct ttttgatttt attttaaatt gttatctgtt tctgtgtata
      181 gactgtttga gatcggcgtt tggccgactc attgtcttac cataggggaa cggactttgt
      241 ttgtgttgtt attttatttg tattttatta aaattctcaa cgatctgaaa aagcctcgcg
      301 gctaagagat tgttgggggg tgagtaagta cttttaaagt gatgatggtt acaaaggcaa
      361 aaggggtaaa acccctcgcc tacgtaagcg ttattacgcc cgtctactta tatcagtaca
      421 ctgacgagtc cctaaaggac gaaacgggag aacgctagcc accaccacca ccaccacgtg
      481 tgaattacag gtgaccagct cgaatttccc cgatcgttca aacatttggc aataaagttt
      541 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta
      601 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat
      661 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa
      721 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat taaactatca
      781 gtgtttgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
      841 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
      901 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
      961 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa
     1021 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
     1081 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
     1141 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
     1201 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
     1261 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
     1321 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
     1381 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
     1441 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
     1501 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
     1561 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
     1621 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
     1681 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
     1741 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
     1801 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
     1861 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg
     1921 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
     1981 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
     2041 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
     2101 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
     2161 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
     2221 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
     2281 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
     2341 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
     2401 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
     2461 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
     2521 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
     2581 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
     2641 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
     2701 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
     2761 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
     2821 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
     2881 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
     2941 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
     3001 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
     3061 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
     3121 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
     3181 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
     3241 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
     3301 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
     3361 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
     3421 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
     3481 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
     3541 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
     3601 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
     3661 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
     3721 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
     3781 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
     3841 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
     3901 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
     3961 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
     4021 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
     4081 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
     4141 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
     4201 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
     4261 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg
     4321 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
     4381 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
     4441 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
     4501 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
     4561 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
     4621 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
     4681 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     4741 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     4801 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     4861 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     4921 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     4981 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5041 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5101 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5161 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5221 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5281 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5341 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5401 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5461 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5521 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agattcttga
     5581 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     5641 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5701 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5761 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5821 ggctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag atacacatct
     5881 tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa tgtgcgaact
     5941 cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa aacgactcaa
     6001 cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc ttaccgaact
     6061 tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa tcgaccgatt
     6121 gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc atgctagctt
     6181 tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata tccgtgagca
     6241 aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg ttactcttta
     6301 tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc aatttctagc
     6361 cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg atgctggctt
     6421 taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa gtcgagtaag
     6481 aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca gcaacttaca
     6541 tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta aaagcaatcc
     6601 aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa atcagcgctc
     6661 gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg caaaggagcc
     6721 atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac ttgttaatat
     6781 ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg
     6841 actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca tgctgctaat
     6901 cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga aacaaggttg
     6961 ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa cagttcgctt
     7021 aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact cactcgtggc
     7081 tgcaaccctg cttactcaaa atctattcac acatggttac gttttgggga aattatgagg
     7141 ggatctctca gcgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca
     7201 gtaaaatata atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata
     7261 gctcgacata ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt
     7321 cataccactt gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat
     7381 ctttcacaaa gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg
     7441 gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt
     7501 cccagttttc gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta
     7561 agcggctgtc taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc
     7621 tgatgcactc cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt
     7681 ccgagcaaag gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt
     7741 caaagtgcag gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt
     7801 cccgttccac atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt
     7861 tttcattttc tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta
     7921 cgcagcggta tttttcgatc agttttttca attccggtga tattctcatt ttagccattt
     7981 attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa
     8041 gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt
     8101 ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc
     8161 gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga
     8221 gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac
     8281 atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg
     8341 ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct
     8401 ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg
     8461 cggacgtttt taatgtactg aattaacgcc gaattaattc ctaggccacc atgttgggcc
     8521 cggcgcgcca agcttgcatg cctgcaggtc aacatggtgg agcacgacac tctcgtctac
     8581 tccaagaata tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa
     8641 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
     8701 aggacagtag aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct
     8761 atcgttcaag atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac
     8821 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatggt
     8881 caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga
     8941 agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt
     9001 ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag atggcttcta
     9061 caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta ccgacagtgg
     9121 tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg ttccaaccac
     9181 gtcttcaaag caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc
     9241 ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggat
     9301 aaaacattgc acctatggtg ttgccctggc tggggtatgt cagtgatcgc agtagaatgt
     9361 actaattgac aagttggaga atacggtaga acgtccttat ccaacacagc ctttatccct
     9421 ctccctgacg aggtttttgt cagtgtaata tttctttttg aactatccag cttagtaccg
     9481 tacgggaaag tgactggtgt gcttatcttt gaaatgttac tttgggtttc ggttctttag
     9541 gttagtaaga aagcacttgt cttctcatac aaaggaaaac ctgagacgta tcgcttacga
     9601 aagtagcaat gaaagaaagg tggtggtttt aatcgctacc gcaaaaacga tggggtcgtt
     9661 ttaattaact tctcctacgc aagcgtctaa acggacgttg gggttttgct agtttcttta
     9721 gagaaaacta gctaagtctt taatgttatc attagagatg gcataaatat aatacttgtg
     9781 tctgctgata agatcatttt aatttggacg attagacttg ttgaactaca ggttactgaa
     9841 tcacttgcgc taatcaacat gggagatatg tacgatgaat catttgacaa gtcgggcggt
     9901 cctgctgact tgatggacga ttcttgggtg gaatcagttt cgtggaaaga tctgttgaag
     9961 aagttacaca gcataaaatt tgcactacag tctggtagag atgagatcac tgggttacta
    10021 gcggcactga atagacagtg tccttattca ccatatgagc agtttccaga taagaaggtg
    10081 tatttccttt tagactcacg ggctaacagt gctcttggtg tgattcagaa cgcttcagcg
    10141 ttcaagagac gagctgatga gaagaatgca gtggcgggtg ttacaaatat tcctgcgaat
    10201 ccaaacacaa cggttacgac gaaccaaggg agtactacta ctaccaaggc gaacactggc
    10261 tcgactttgg aagaagactt gtacacttat tacaaattcg atgatgcctc tacagctttc
    10321 cacaaatctc taacttcgtt agagaacatg gagttgaaga gttattaccg aaggaacttt
    10381 gagaaagtat tcgggattaa gtttggtgga gcagctgcta gttcatctgc accgcctcca
    10441 gcgagtggag gtccgatacg tcctaatccc tagggattta aggacgtgaa ctctgttgag
    10501 atctctgtga aattcagagg gtgggtgata ccatattcac tgatgccatt agcgacatct
    10561 aaatagggct aattgtgact aatttgaggg aatttccttt accattgacg tcagtgtcgt
    10621 tggtagcatt tgagtttcgg agcatcttgt tctggggttt cacactatct ttagagaaag
    10681 tgttaagtta attaagttat cttaattaag agcataatta tactgatttg tctctcgttg
    10741 atagagtcta tcattctgtt actaaaaatt tgacaactcg gtttgctgac ctactggtta
    10801 ctgtatcact tacccgagtt aacgagtcta gaaacatgaa aagaactgct gatggatcag
    10861 aatttgaaag cccgaaaaaa aaaagaaagg tttcaatgtc tgagccacct cgggctgaga
    10921 cctttgtatt cctggaccta gaagccactg ggctcccaaa catggaccct gagattgcag
    10981 agatatccct ttttgctgtt caccgctctt ccctggagaa cccagaacgg gatgattctg
    11041 gttccttggt gctgccccgt gttctggaca agctcacact gtgcatgtgc ccggagcgcc
    11101 cctttactgc caaggccagt gagattactg gtttgagcag cgaaagcctg atgcactgcg
    11161 ggaaggctgg tttcaatggc gctgtggtaa ggacactgca gggcttccta agccgccagg
    11221 agggccccat ctgccttgtg gcccacaatg gcttcgatta tgacttccca ctgctgtgca
    11281 cggagctaca acgtctgggt gcccatctgc cccaagacac tgtctgcctg gacacactgc
    11341 ctgcattgcg gggcctggac cgtgctcaca gccacggcac cagggctcaa ggccgcaaaa
    11401 gctacagcct ggccagtctc ttccaccgct acttccaggc tgaacccagt gctgcccatt
    11461 cagcagaagg tgatgtgcac accctgcttc tgatcttcct gcatcgtgct cctgagctgc
    11521 tcgcctgggc agatgagcag gcccgcagct gggctcatat tgagcccatg tacgtgccac
    11581 ctgatggtcc aagcctcgaa gcctaatccc ttggtagtag cgactccatg gtttaagagc
    11641 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
    11701 ggcaccgagt cggtgctccc gctcccgtag ctcagttggt tagagcgttg gtcttatgag
    11761 ccgaaggtcg cgggttcgag ccccgccgga agcaagtg
//
