LOCUS       sequence-458104-       11339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNA targeting SlPDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11339)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 11339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pEE834
FEATURES             Location/Qualifiers
     source          1..11339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1141..1935)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    2360..2384
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2913..3258
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     3201..3221
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        5399..5474
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             6046..6063
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6098..6350
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6372..6396
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7697..8326
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8755..9828
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9894..10088
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10324..10346)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10432..10572
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10487..10506
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10587..10604)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(10758..11339,1..7))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10838..10857)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
       61 acgttaaggg ctgatgaatc ccctaatgat ttttatcaaa atcattaagt taaggtagat
      121 acacatcttg tcatatgatc aaatggtttc gccaaaaatc aataatcaga caacaaaatg
      181 tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa
      241 cgactcaaca gcttaacgtt ggcttgccac gccttacttg actgtaaaac tctcactctt
      301 accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc
      361 gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat
      421 gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc
      481 cgtgagcaaa aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt
      541 actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa
      601 tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat
      661 gctggcttta aagtgccatg gattctagca actaacttac ctgttgaaat tcgaacaccc
      721 aaacaacttg ttaatatcta ttcgaagcga atgcagattg aagaaacctt ccgagacttg
      781 aaaagtcctg cctacggact aggcctacgc catagccgaa cgagcagctc agagcgtttt
      841 gatatcatgc tgctaatcgc cctgatgctt caactaacat gttggcttgc gggcgttcat
      901 gctcagaaac aaggttggga caagcacttc caggctaaca cagtcagaaa tcgaaacgta
      961 ctctcaacag ttcgcttagg catggaagtt ttgcggcatt ctggctacac aataacaagg
     1021 gaagactcac tcgtggctgc aaccctgctt actcaaaatc tattcacaca tggttacgtt
     1081 ttggggaaat tatgagggga tctctcagcg ttaagggatt ttggtcatgc attctaggta
     1141 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     1201 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     1261 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     1321 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     1381 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     1441 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     1501 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     1561 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     1621 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     1681 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     1741 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     1801 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     1861 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     1921 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     1981 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     2041 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     2101 gagccgattt tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa
     2161 catgctaccc tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc
     2221 cgaatagcat cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc
     2281 cgtcccggac tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg
     2341 ggagctgttg gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac
     2401 aacttaataa cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattccta
     2461 ggccaccatg ttgggcccgg cgcgccaagc ttgcatgcct gcaggtcaac atggtggagc
     2521 acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta
     2581 ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta
     2641 tctgtcactt catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt
     2701 gcgataaagg aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac
     2761 ccccacccac gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     2821 tggattgatg tgatggtcaa catggtggag cacgacactc tcgtctactc caagaatatc
     2881 aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag ggtaatatcg
     2941 ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa
     3001 aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat
     3061 gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat cgtggaaaaa
     3121 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
     3181 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
     3241 tttcatttgg agaggataaa acattgcacc tatggtgttg ccctggctgg ggtatgtcag
     3301 tgatcgcagt agaatgtact aattgacaag ttggagaata cggtagaacg tccttatcca
     3361 acacagcctt tatccctctc cctgacgagg tttttgtcag tgtaatattt ctttttgaac
     3421 tatccagctt agtaccgtac gggaaagtga ctggtgtgct tatctttgaa atgttacttt
     3481 gggtttcggt tctttaggtt agtaagaaag cacttgtctt ctcatacaaa ggaaaacctg
     3541 agacgtatcg cttacgaaag tagcaatgaa agaaaggtgg tggttttaat cgctaccgca
     3601 aaaacgatgg ggtcgtttta attaacttct cctacgcaag cgtctaaacg gacgttgggg
     3661 ttttgctagt ttctttagag aaaactagct aagtctttaa tgttatcatt agagatggca
     3721 taaatataat acttgtgtct gctgataaga tcattttaat ttggacgatt agacttgttg
     3781 aactacaggt tactgaatca cttgcgctaa tcaacatggg agatatgtac gatgaatcat
     3841 ttgacaagtc gggcggtcct gctgacttga tggacgattc ttgggtggaa tcagtttcgt
     3901 ggaaagatct gttgaagaag ttacacagca taaaatttgc actacagtct ggtagagatg
     3961 agatcactgg gttactagcg gcactgaata gacagtgtcc ttattcacca tatgagcagt
     4021 ttccagataa gaaggtgtat ttccttttag actcacgggc taacagtgct cttggtgtga
     4081 ttcagaacgc ttcagcgttc aagagacgag ctgatgagaa gaatgcagtg gcgggtgtta
     4141 caaatattcc tgcgaatcca aacacaacgg ttacgacgaa ccaagggagt actactacta
     4201 ccaaggcgaa cactggctcg actttggaag aagacttgta cacttattac aaattcgatg
     4261 atgcctctac agctttccac aaatctctaa cttcgttaga gaacatggag ttgaagagtt
     4321 attaccgaag gaactttgag aaagtattcg ggattaagtt tggtggagca gctgctagtt
     4381 catctgcacc gcctccagcg agtggaggtc cgatacgtcc taatccctag ggatttaagg
     4441 acgtgaactc tgttgagatc tctgtgaaat tcagagggtg ggtgatacca tattcactga
     4501 tgccattagc gacatctaaa tagggctaat tgtgactaat ttgagggaat ttcctttacc
     4561 attgacgtca gtgtcgttgg tagcatttga gtttcggagc atcttgttct ggggtttcac
     4621 actatcttta gagaaagtgt taagttaatt aagttatctt aattaagagc ataattatac
     4681 tgatttgtct ctcgttgata gagtctatca ttctgttact aaaaatttga caactcggtt
     4741 tgctgaccta ctggttactg tatcacttac ccgagttaac gagtctagaa acgaacatca
     4801 acacaacata gaagatggtg gtaaaaatag taacaacagt ttcctgtgca ggcaaagtag
     4861 tagccgttgg acgccaacga gcgatcagat aagaatattg aaggatctct actacaacaa
     4921 tggagttagg tctccaactg ctgaacagat tcagaggata tctgctaagt tgagacagta
     4981 cggtaagatt gaaggcaaaa atgtgtttta ttggtttcag aaccataaag ctcgtgaaag
     5041 acaaaagaag aggctcattg ctgctgcctc tgccactgat aataataata tctcttccat
     5101 gcaaatgatt ccacatcttt ggagatctcc tgatgatcac cacaagtaca acactactac
     5161 tactaatcca ggtaattctt tctacttttt agtattgtct tggttaaaat aaagcggact
     5221 acctgctgct ataacatgtt aaaatacatt aatagtttac gattatatat atacaggtgt
     5281 tcagtgtcca tcaccatctt cacatggggt attaccagtg gtacagactg gaaactatgg
     5341 ttatggaact ttggctatgg agaagagctt tagggtgcgg actcttgcca gcaatgctgt
     5401 tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg
     5461 caccgagtcg gtgctcccta gtctataaat ataagagacc ctcttatagt aagcagagtt
     5521 gttggagacg ttcttgatcc gtttaataga tcaatcactc taaaggttac ttatggccaa
     5581 agtgccgggc atgtcccgaa gacattaaac tacggttctt taagtagatc cgtgtctgaa
     5641 gttttaggtt caatttaaac ctacgagatt gacattctcg actgatcttg attgatcggt
     5701 aagtcttttg taatttaatt ttctttttga ttttatttta aattgttatc tgtttctgtg
     5761 tatagactgt ttgagatcgg cgtttggccg actcattgtc ttaccatagg ggaacggact
     5821 ttgtttgtgt tgttatttta tttgtatttt attaaaattc tcaacgatct gaaaaagcct
     5881 cgcggctaag agattgttgg ggggtgagta agtactttta aagtgatgat ggttacaaag
     5941 gcaaaagggg taaaacccct cgcctacgta agcgttatta cgcccgtctg tacttatatc
     6001 agtacactga cgagtcccta aaggacgaaa cgggagaacg ctagccacca ccaccaccac
     6061 cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata
     6121 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     6181 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     6241 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     6301 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa
     6361 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
     6421 aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     6481 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     6541 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     6601 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     6661 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     6721 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     6781 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     6841 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     6901 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     6961 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     7021 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     7081 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     7141 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     7201 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     7261 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     7321 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     7381 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     7441 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     7501 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     7561 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     7621 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     7681 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     7741 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     7801 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     7861 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     7921 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     7981 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     8041 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     8101 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     8161 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     8221 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     8281 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     8341 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     8401 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     8461 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     8521 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     8581 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     8641 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     8701 ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     8761 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     8821 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     8881 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     8941 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     9001 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     9061 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     9121 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     9181 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     9241 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     9301 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     9361 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     9421 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     9481 atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     9541 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     9601 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     9661 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     9721 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     9781 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     9841 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat
     9901 agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac
     9961 ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa
    10021 ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc
    10081 tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg
    10141 tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca
    10201 aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca
    10261 ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga
    10321 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
    10381 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    10441 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    10501 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    10561 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10621 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10681 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10741 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10801 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10861 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10921 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10981 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    11041 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    11101 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    11161 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    11221 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    11281 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagg
//
