LOCUS       sequence-458119-       10725 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNA targeting SlPDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pEE771
FEATURES             Location/Qualifiers
     source          1..10725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    22..46
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        575..920
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     863..883
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_RNA        2475..2550
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             3094..3111
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3146..3398
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3420..3444
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4745..5374
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5803..6876
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6942..7136
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7372..7394)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7480..7620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7535..7554
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7635..7652)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7806..8394)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7886..7905)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9528..10322)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag
       61 acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcc
      121 taggccacca tgttgggccc ggcgcgccaa gcttgcatgc ctgcaggtca acatggtgga
      181 gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag accagagggc
      241 tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc
      301 tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca aatgccatca
      361 ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc ccaaagatgg
      421 acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      481 agtggattga tgtgatggtc aacatggtgg agcacgacac tctcgtctac tccaagaata
      541 tcaaagatac agtctcagaa gaccagaggg ctattgagac ttttcaacaa agggtaatat
      601 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      661 aaaaggaaga tggcttctac aaatgccatc attgcgataa aggaaaggct atcgttcaag
      721 atgcctctac cgacagtggt cccaaagatg gacccccacc cacgaggaac atcgtggaaa
      781 aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc tccactgacg
      841 taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata taaggaagtt
      901 catttcattt ggagaggata aaacattgca cctatggtgt tgccctggct ggggtatgtc
      961 agtgatcgca gtagaatgta ctaattgaca agttggagaa tacggtagaa cgtccttatc
     1021 caacacagcc tttatccctc tccctgacga ggtttttgtc agtgtaatat ttctttttga
     1081 actatccagc ttagtaccgt acgggaaagt gactggtgtg cttatctttg aaatgttact
     1141 ttgggtttcg gttctttagg ttagtaagaa agcacttgtc ttctcataca aaggaaaacc
     1201 tgagacgtat cgcttacgaa agtagcaatg aaagaaaggt ggtggtttta atcgctaccg
     1261 caaaaacgat ggggtcgttt taattaactt ctcctacgca agcgtctaaa cggacgttgg
     1321 ggttttgcta gtttctttag agaaaactag ctaagtcttt aatgttatca ttagagatgg
     1381 cataaatata atacttgtgt ctgctgataa gatcatttta atttggacga ttagacttgt
     1441 tgaactacag gttactgaat cacttgcgct aatcaacatg ggagatatgt acgatgaatc
     1501 atttgacaag tcgggcggtc ctgctgactt gatggacgat tcttgggtgg aatcagtttc
     1561 gtggaaagat ctgttgaaga agttacacag cataaaattt gcactacagt ctggtagaga
     1621 tgagatcact gggttactag cggcactgaa tagacagtgt ccttattcac catatgagca
     1681 gtttccagat aagaaggtgt atttcctttt agactcacgg gctaacagtg ctcttggtgt
     1741 gattcagaac gcttcagcgt tcaagagacg agctgatgag aagaatgcag tggcgggtgt
     1801 tacaaatatt cctgcgaatc caaacacaac ggttacgacg aaccaaggga gtactactac
     1861 taccaaggcg aacactggct cgactttgga agaagacttg tacacttatt acaaattcga
     1921 tgatgcctct acagctttcc acaaatctct aacttcgtta gagaacatgg agttgaagag
     1981 ttattaccga aggaactttg agaaagtatt cgggattaag tttggtggag cagctgctag
     2041 ttcatctgca ccgcctccag cgagtggagg tccgatacgt cctaatccct agggatttaa
     2101 ggacgtgaac tctgttgaga tctctgtgaa attcagaggg tgggtgatac catattcact
     2161 gatgccatta gcgacatcta aatagggcta attgtgacta atttgaggga atttccttta
     2221 ccattgacgt cagtgtcgtt ggtagcattt gagtttcgga gcatcttgtt ctggggtttc
     2281 acactatctt tagagaaagt gttaagttaa ttaagttatc ttaattaaga gcataattat
     2341 actgatttgt ctctcgttga tagagtctat cattctgtta ctaaaaattt gacaactcgg
     2401 tttgctgacc tactggttac tgtatcactt acccgagtta acgagtctag aaacggactc
     2461 ttgccagcaa tgctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     2521 caacttgaaa aagtggcacc gagtcggtgc tcccgctccc gtagctcagt tggttagagc
     2581 gttggtctta tgagccgaag gtcgcgggtt cgagccccgc cggaagcaag tgccgggcat
     2641 gtcccgaaga cattaaacta cggttcttta agtagatccg tgtctgaagt tttaggttca
     2701 atttaaacct acgagattga cattctcgac tgatcttgat tgatcggtaa gtcttttgta
     2761 atttaatttt ctttttgatt ttattttaaa ttgttatctg tttctgtgta tagactgttt
     2821 gagatcggcg tttggccgac tcattgtctt accatagggg aacggacttt gtttgtgttg
     2881 ttattttatt tgtattttat taaaattctc aacgatctga aaaagcctcg cggctaagag
     2941 attgttgggg ggtgagtaag tacttttaaa gtgatgatgg ttacaaaggc aaaaggggta
     3001 aaacccctcg cctacgtaag cgttattacg cccgtctgta cttatatcag tacactgacg
     3061 agtccctaaa ggacgaaacg ggagaacgct agccaccacc accaccacca cgtgtgaatt
     3121 acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     3181 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     3241 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     3301 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     3361 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact atcagtgttt
     3421 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
     3481 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     3541 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     3601 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     3661 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     3721 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     3781 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     3841 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     3901 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     3961 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     4021 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     4081 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4141 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4201 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4261 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4321 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     4381 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     4441 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     4501 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     4561 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     4621 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     4681 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     4741 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     4801 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     4861 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     4921 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     4981 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     5041 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     5101 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5161 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5221 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5281 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     5341 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     5401 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     5461 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     5521 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     5581 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     5641 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     5701 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgcctgccg
     5761 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     5821 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     5881 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     5941 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg
     6001 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     6061 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     6121 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     6181 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     6241 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     6301 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     6361 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     6421 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     6481 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     6541 gattggatgt accgcgagat cacagaaggc aagaacccgg acgtgctgac ggttcacccc
     6601 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     6661 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     6721 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     6781 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     6841 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     6901 attgccctag caggggaaaa aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt
     6961 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     7021 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     7081 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     7141 tctggccagc gcacagccga agagctgcaa aaagcgccta cccttcggtc gctgcgctcc
     7201 ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg gccgctcaaa aatggctggc
     7261 ctacggccag gcaatctacc agggcgcgga caagccgcgc cgtcgccact cgaccgccgg
     7321 cgcccacatc aaggcaccct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     7381 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     7441 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     7501 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7561 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7621 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7681 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7741 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7801 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7861 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7921 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7981 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8041 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8101 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8161 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8221 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     8281 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8341 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8401 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggctg
     8461 atgaatcccc taatgatttt tatcaaaatc attaagttaa ggtagataca catcttgtca
     8521 tatgatcaaa tggtttcgcc aaaaatcaat aatcagacaa caaaatgtgc gaactcgata
     8581 ttttacacga ctctctttac caattctgcc ccgaattaca cttaaaacga ctcaacagct
     8641 taacgttggc ttgccacgcc ttacttgact gtaaaactct cactcttacc gaacttggcc
     8701 gtaacctgcc aaccaaagcg agaacaaaac ataacatcaa acgaatcgac cgattgttag
     8761 gtaatcgtca cctccacaaa gagcgactcg ctgtataccg ttggcatgct agctttatct
     8821 gttcgggcaa tacgatgccc attgtacttg ttgactggtc tgatatccgt gagcaaaaac
     8881 ggcttatggt attgcgagct tcagtcgcac tacacggtcg ttctgttact ctttatgaga
     8941 aagcgttccc gctttcagag caatgttcaa agaaagctca tgaccaattt ctagccgacc
     9001 ttgcgagcat tctaccgagt aacaccacac cgctcattgt cagtgatgct ggctttaaag
     9061 tgccatggat tctagcaact aacttacctg ttgaaattcg aacacccaaa caacttgtta
     9121 atatctattc gaagcgaatg cagattgaag aaaccttccg agacttgaaa agtcctgcct
     9181 acggactagg cctacgccat agccgaacga gcagctcaga gcgttttgat atcatgctgc
     9241 taatcgccct gatgcttcaa ctaacatgtt ggcttgcggg cgttcatgct cagaaacaag
     9301 gttgggacaa gcacttccag gctaacacag tcagaaatcg aaacgtactc tcaacagttc
     9361 gcttaggcat ggaagttttg cggcattctg gctacacaat aacaagggaa gactcactcg
     9421 tggctgcaac cctgcttact caaaatctat tcacacatgg ttacgttttg gggaaattat
     9481 gaggggatct ctcagcgtta agggattttg gtcatgcatt ctaggtacta aaacaattca
     9541 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
     9601 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
     9661 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
     9721 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
     9781 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
     9841 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
     9901 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
     9961 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
    10021 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
    10081 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
    10141 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
    10201 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
    10261 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
    10321 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
    10381 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
    10441 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
    10501 aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat gctaccctcc
    10561 gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga atagcatcgg
    10621 taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt cccggactga
    10681 tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtc
//
