LOCUS       sequence-458122-       18202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPR/Cas9 genome editing vector pKSEe401R contains the maize 
            codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of 
            OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18202)
  AUTHORS   Kiryushkin AS, Ilina EL, Kiikova TY, Pawlowski K, Demchenko KN
  TITLE     Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle 
            in Cucumber (Cucumis sativus)?
  JOURNAL   Int J Mol Sci. 2024 Feb 6;25(4):1975. doi: 10.3390/ijms25041975.
  PUBMED    38396652
REFERENCE   2  (bases 1 to 18202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Int J Mol 
            Sci."; date: "2024-02-6"; volume: "25(4):1975. doi"; pages: " 
            10.3390/ijms25041975"
COMMENT     Sequence Label: pKSEe401R
FEATURES             Location/Qualifiers
     source          1..18202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             977..1768
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1920..1995
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2609..2953
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3265..3330
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3337..3357
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3382..7482
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3382..7482
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7395..7403
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7483..7530
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      complement(8181..8433)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(8446..9120,9121..9123,9124..9126))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MARSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(8508..8531)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(8706..8725)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8923..8943
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     complement(9793..9809)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9793..9809)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9806..9828)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9817..9833
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9841..9847,9848..9865,9866..9871))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9886..9907
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10098..10774
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             10837..11634
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10894..10913)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11504..11523
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    11691..11865
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11943..11967
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12392..13186
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13273..13861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13762..13781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14015..14032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14047..14187
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14113..14132)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14273..14295
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      14531..14725
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(14791..15864)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16293..16922)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttcgacttg ccttccgcac aatacatcat
      301 ttcttcttag ctttttttct tcttcttcgt tcatacagtt tttttttgtt tatcagctta
      361 cattttcttg aaccgtagct ttcgttttct tctttttaac tttccattcg gagtttttgt
      421 atcttgtttc atagtttgtc ccaggattag aatgattagg catcgaacct tcaagaattt
      481 gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag gcccctggga
      541 atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat ttcttatata
      601 ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata agaaaacgaa
      661 gctgagttta tatacagcta gagtcgaagt agtgattggg agaccaaccc agtggacata
      721 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      781 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      841 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
      901 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
      961 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1021 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1081 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     1141 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1201 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     1261 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     1321 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     1381 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     1441 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     1501 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     1561 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     1621 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     1681 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     1741 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     1801 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     1861 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccggtctcgg
     1921 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1981 gcaccgagtc ggtgcttttt tttgcaaaat tttccagatc gatttcttct tcctctgttc
     2041 ttcggcgttc aatttctggg gttttctctt cgttttctgt aactgaaacc taaaatttga
     2101 cctaaaaaaa atctcaaata atatgattca gtggttttgt acttttcagt tagttgagtt
     2161 ttgcagttcc gatgagataa accaataagc ttgcatgcct gcaggtcaac atggtggagc
     2221 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     2281 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     2341 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     2401 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     2461 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     2521 tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
     2581 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
     2641 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
     2701 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
     2761 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     2821 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     2881 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     2941 atttggagag gacctcgacc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
     3001 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
     3061 tgaaaatttt caccatttac gaacgatact cgagtaatct agaaagacta aagagagctt
     3121 tttcatacca aagaagtaca acaaaagatt tgctcctcat tttctgaatc ctgggactct
     3181 ctagcctgta gaagaagaaa ggcaggaatt tcagctcaag agaacagatc acaatattta
     3241 cccacggcac tgtctcgcaa tatggattac aaggaccacg acggggatta caaggaccac
     3301 gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag gaaggttggc
     3361 atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgatat tgggactaac
     3421 tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa gttcaaggtc
     3481 ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct cctgttcgac
     3541 agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg gtacacgcgc
     3601 aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc gaaggttgac
     3661 gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa gaagcacgag
     3721 cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa gtaccctacg
     3781 atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct ccgcctgatc
     3841 tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga gggggatctc
     3901 aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac gtacaaccag
     3961 ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct
     4021 aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg cgagaagaag
     4081 aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa cttcaagtcg
     4141 aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata cgacgatgac
     4201 ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct cgctgccaag
     4261 aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga gattacgaag
     4321 gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga tctgaccctc
     4381 ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt cttcgatcag
     4441 tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga gttctacaag
     4501 ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt gaagctcaat
     4561 cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc acaccagatt
     4621 catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctatcc tttcctcaag
     4681 gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta cgtcggccca
     4741 ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga gacgatcacg
     4801 ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg
     4861 atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca ctcgctcctg
     4921 tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac cgagggcatg
     4981 aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct cctgttcaag
     5041 accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa gattgagtgc
     5101 ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct ggggacctac
     5161 cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga gaatgaggat
     5221 atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat gatcgaggag
     5281 cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct caagaggagg
     5341 aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg ggacaagcag
     5401 tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg caatttcatg
     5461 cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc tcaggtgagc
     5521 ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc agctatcaag
     5581 aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac
     5641 aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca gaagggccag
     5701 aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct ggggtcccag
     5761 atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct ctacctgtac
     5821 tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa caggctcagc
     5881 gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc cattgacaac
     5941 aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc ttcagaggag
     6001 gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct gatcacgcag
     6061 cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct ggacaaggcg
     6121 ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt
     6181 ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg cgaggtgaag
     6241 gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca gttctacaag
     6301 gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc ggtggtcggc
     6361 acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg ggactacaag
     6421 gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa ggctaccgcc
     6481 aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac tctggccaat
     6541 ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga gatcgtgtgg
     6601 gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca agtgaatatc
     6661 gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct gcctaagcgg
     6721 aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta cggcgggttc
     6781 gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa gggcaagtcc
     6841 aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg ctccagcttc
     6901 gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa gaaggacctg
     6961 atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa gcggatgctg
     7021 gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa gtatgtgaac
     7081 ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga caacgagcag
     7141 aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga gcagatttcc
     7201 gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct ctccgcgtac
     7261 aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca tctcttcacc
     7321 ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat cgatcgcaag
     7381 aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc gattaccggc
     7441 ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc agcggcgacg
     7501 aagaaggcgg ggcaggcgaa gaagaagaag tgagagctca gagctttcgt tcgtatcatc
     7561 ggtttcgaca acgttcgtca agttcaatgc atcagtttca ttgcgcacac accagaatcc
     7621 tactgagttt gagtattatg gcattgggaa aactgttttt cttgtaccat ttgttgtgct
     7681 tgtaatttac tgtgtttttt attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg
     7741 gagaagagtt aatgaatgat atggtccttt tgttcattct caaattaata ttatttgttt
     7801 tttctcttat ttgttgtgtg ttgaatttga aattataaga gatatgcaaa cattttgttt
     7861 tgagtaaaaa tgtgtcaaat cgtggcctct aatgaccgaa gttaatatga ggagtaaaac
     7921 acttgtagtt gtaccattat gcttattcac taggcaacaa atatattttc agacctagaa
     7981 aagctgcaaa tgttactgaa tacaagtatg tcctcttgtg ttttagacat ttatgaactt
     8041 tcctttatgt aattttccag aatccttgtc agattctaat cattgcttta taattatagt
     8101 tatactcatg gatttgtagt tgagtatgaa aatatttttt aatgcatttt atgacttgcc
     8161 aattgattga caacgaattc gatctagtaa catagatgac accgcgcgcg ataatttatc
     8221 ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct
     8281 aatcataaaa acccatctca taaataacgt catgcattac atgttaatta ttacatgctt
     8341 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
     8401 taagaaactt tattgccaaa tgtttgaacg atcgggaatt ggatcctaca ggaacaggtg
     8461 gtggcggccc tcggtgcgct cgtactgctc cacgatggtg tagtcctcgt tgtgggaggt
     8521 gatgtccagc ttggagtcca cgtagtagta gccgggcagc tgcacgggct tcttggccat
     8581 gtagatggac ttgaactcca ccaggtagtg gccgccgtcc ttcagcttca gggccttgtg
     8641 gatctcgccc ttcagcacgc cgtcgcgggg gtacaggcgc tcggtggagg cctcccagcc
     8701 catggtcttc ttctgcatta cggggccgtc ggaggggaag ttcacgccga tgaacttcac
     8761 cttgtagatg aagcagccgt cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc
     8821 gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca gcttcttgta
     8881 gtcggggatg tcggcggggt gcttcacgta caccttggag ccgtactgga actgggggga
     8941 caggatgtcc caggcgaagg gcagggggcc gcccttggtc accttcagct tcacggtgtt
     9001 gtggccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt ggccgttcac
     9061 ggtgccctcc atgcgcacct tgaagcgcat gaactccttg atgacgttct tggaggagcg
     9121 cgccatggca aagatctgca tctgttaatc agaaaaactc agattaatcg acaaattcga
     9181 tcgcacaaac tagaaactaa caccagatct agatagaaat cacaaatcga agagtaatta
     9241 ttcgacaaaa ctcaaattat ttgaacaaat cggatgatat ctatgaaacc ctaatcgaga
     9301 attaagatga tatctaacga tcaaacccag aaaatcgtct tcgatctaag attaacagaa
     9361 tctaaaccaa agaacatata cgaaattggg atcgaacgaa aacaaaatcg aagattttga
     9421 gagaataagg aacacagaaa tttaccttga tcacggtaga gagaattgag agaaagtttt
     9481 taagattttg agaaattgaa atctgaattg tgaagaagaa gagctctttg ggtattgttt
     9541 tatagaagaa gaagaagaaa agacgaggac gactaggtca cgagaaagct aaggcggtga
     9601 agcaatagct aataataaaa tgacacgtgt attgagcgtt gtttacacgc aaagttgttt
     9661 ttggctaatt gccttatttt taggttgagg aaaagtattt gtgctttgag ttgataaaca
     9721 cgactcgtgt gtgccggctg caaccacttt gacgccgttt attactggac ttcgtccgga
     9781 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9841 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9901 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
    10021 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
    10081 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
    10141 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
    10201 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
    10261 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
    10321 acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc tcgtctactc
    10381 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    10441 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
    10501 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
    10561 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
    10621 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
    10681 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
    10741 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
    10801 atctctctcg agctttcgca gatctgtcga tcgaccatgg ggattgaaca agatggattg
    10861 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    10921 acaatcggct gctctgatgc cgccgtgttt cggctgtcag cgcaggggcg cccggttctt
    10981 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta
    11041 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    11101 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    11161 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    11221 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    11281 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    11341 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgaca
    11401 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    11461 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    11521 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    11581 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
    11641 ctctggggtt cggatcgatc ctctagctag agtcgatcga caagctcgag tttctccata
    11701 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
    11761 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    11821 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaattcg
    11881 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
    11941 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
    12001 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
    12061 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
    12121 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
    12181 gacagagcgt tgctgcctgt gatcaccgcg gtttcaaaat cggctccgtc gatactatgt
    12241 tatacgccaa ctttgaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga
    12301 atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta
    12361 aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt
    12421 gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa
    12481 ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta
    12541 taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa
    12601 gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat
    12661 gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa
    12721 gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg
    12781 tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga
    12841 tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga
    12901 gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga
    12961 cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg
    13021 gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga
    13081 ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga
    13141 ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtacc tagaatgcat
    13201 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    13261 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    13321 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    13381 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
    13441 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    13501 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    13561 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    13621 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    13681 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    13741 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    13801 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    13861 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    13921 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    13981 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    14041 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
    14101 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
    14161 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
    14221 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
    14281 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
    14341 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
    14401 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
    14461 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
    14521 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
    14581 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
    14641 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
    14701 gttcccaatg tacgtgctat ccacaggaaa cagacctttt cgaccttttt cccctgctag
    14761 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
    14821 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
    14881 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
    14941 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
    15001 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
    15061 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
    15121 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
    15181 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
    15241 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
    15301 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
    15361 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
    15421 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    15481 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    15541 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    15601 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    15661 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    15721 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    15781 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    15841 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    15901 cagggccggc aggcaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    15961 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    16021 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    16081 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    16141 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    16201 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    16261 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    16321 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    16381 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    16441 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    16501 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    16561 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    16621 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    16681 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    16741 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    16801 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    16861 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    16921 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    16981 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    17041 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    17101 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    17161 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    17221 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    17281 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    17341 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    17401 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    17461 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    17521 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    17581 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    17641 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    17701 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    17761 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    17821 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    17881 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    17941 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    18001 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    18061 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    18121 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt ttcacgccct
    18181 tttaaatatc cgattattct aa
//
