LOCUS       sequence-458130-       11026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNA targeting SlPDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11026)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 11026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pDL518
FEATURES             Location/Qualifiers
     source          1..11026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             462..479
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      514..766
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    788..812
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2113..2742
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3171..4244
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4310..4504
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4740..4762)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4848..4988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4903..4922
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5003..5020)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5174..5762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5254..5273)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7187..7981)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8406..8430
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8959..9304
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9247..9267
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ccgggcatgt cccgaagaca ttaaactacg gttctttaag tagatccgtg tctgaagttt
       61 taggttcaat ttaaacctac gagattgaca ttctcgactg atcttgattg atcggtaagt
      121 cttttgtaat ttaattttct ttttgatttt attttaaatt gttatctgtt tctgtgtata
      181 gactgtttga gatcggcgtt tggccgactc attgtcttac cataggggaa cggactttgt
      241 ttgtgttgtt attttatttg tattttatta aaattctcaa cgatctgaaa aagcctcgcg
      301 gctaagagat tgttgggggg tgagtaagta cttttaaagt gatgatggtt acaaaggcaa
      361 aaggggtaaa acccctcgcc tacgtaagcg ttattacgcc cgtctgtact tatatcagta
      421 cactgacgag tccctaaagg acgaaacggg agaacgctag ccaccaccac caccaccacg
      481 tgtgaattac aggtgaccag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
      541 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
      601 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
      661 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
      721 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attaaactat
      781 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
      841 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
      901 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
      961 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     1021 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     1081 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     1141 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     1201 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     1261 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     1321 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     1381 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     1441 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     1501 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     1561 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     1621 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     1681 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     1741 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     1801 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     1861 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     1921 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     1981 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     2041 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     2101 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     2161 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     2221 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     2281 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     2341 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     2401 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     2461 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     2521 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     2581 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     2641 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     2701 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     2761 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     2821 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     2881 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     2941 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     3001 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     3061 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     3121 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     3181 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     3241 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     3301 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     3361 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     3421 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     3481 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     3541 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     3601 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     3661 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     3721 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     3781 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     3841 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     3901 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     3961 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     4021 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     4081 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     4141 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     4201 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     4261 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tctctttcct gtggatagca
     4321 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
     4381 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
     4441 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
     4501 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
     4561 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
     4621 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
     4681 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     4741 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4801 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     4861 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     4921 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4981 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5041 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5101 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5161 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5221 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5281 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5341 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5401 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5461 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5521 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5581 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     5641 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5701 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5761 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5821 aagggctgat gaatccccta atgattttta tcaaaatcat taagttaagg tagatacaca
     5881 tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga
     5941 actcgatatt ttacacgact ctctttacca attctgcccc gaattacact taaaacgact
     6001 caacagctta acgttggctt gccacgcctt acttgactgt aaaactctca ctcttaccga
     6061 acttggccgt aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg
     6121 attgttaggt aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag
     6181 ctttatctgt tcgggcaata cgatgcccat tgtacttgtt gactggtctg atatccgtga
     6241 gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct
     6301 ttatgagaaa gcgttcccgc tttcagagca atgttcaaag aaagctcatg accaatttct
     6361 agccgacctt gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg
     6421 ctttaaagtg ccatggtata aatccgttga gaagctgggt tggtactggt taagtcgagt
     6481 aagaggaaaa gtacaatatg cagacctagg agcggaaaac tggaaaccta tcagcaactt
     6541 acatgatatg tcatctagtc actcaaagac tttaggctat aagaggctga ctaaaagcaa
     6601 tccaatctca tgccaaattc tattgtataa atctcgctct aaaggccgaa aaaatcagcg
     6661 ctcgacacgg actcattgtc accacccgtc acctaaaatc tactcagcgt cggcaaagga
     6721 gccatggatt ctagcaacta acttacctgt tgaaattcga acacccaaac aacttgttaa
     6781 tatctattcg aagcgaatgc agattgaaga aaccttccga gacttgaaaa gtcctgccta
     6841 cggactaggc ctacgccata gccgaacgag cagctcagag cgttttgata tcatgctgct
     6901 aatcgccctg atgcttcaac taacatgttg gcttgcgggc gttcatgctc agaaacaagg
     6961 ttgggacaag cacttccagg ctaacacagt cagaaatcga aacgtactct caacagttcg
     7021 cttaggcatg gaagttttgc ggcattctgg ctacacaata acaagggaag actcactcgt
     7081 ggctgcaacc ctgcttactc aaaatctatt cacacatggt tacgttttgg ggaaattatg
     7141 aggggatctc tcagcgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     7201 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     7261 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     7321 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     7381 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     7441 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     7501 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     7561 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     7621 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     7681 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     7741 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     7801 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     7861 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     7921 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     7981 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     8041 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     8101 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     8161 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     8221 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     8281 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     8341 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     8401 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     8461 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcctaggcc accatgttgg
     8521 gcccggcgcg ccaagcttgc atgcctgcag gtcaacatgg tggagcacga cactctcgtc
     8581 tactccaaga atatcaaaga tacagtctca gaagaccaga gggctattga gacttttcaa
     8641 caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc
     8701 gaaaggacag tagaaaagga agatggcttc tacaaatgcc atcattgcga taaaggaaag
     8761 gctatcgttc aagatgcctc taccgacagt ggtcccaaag atggaccccc acccacgagg
     8821 aacatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
     8881 ggtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
     8941 agaagaccag agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
     9001 attccattgc ccagctatct gtcacttcat cgaaaggaca gtagaaaagg aagatggctt
     9061 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctaccgacag
     9121 tggtcccaaa gatggacccc cacccacgag gaacatcgtg gaaaaagaag acgttccaac
     9181 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
     9241 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     9301 gataaaacat tgcacctatg gtgttgccct ggctggggta tgtcagtgat cgcagtagaa
     9361 tgtactaatt gacaagttgg agaatacggt agaacgtcct tatccaacac agcctttatc
     9421 cctctccctg acgaggtttt tgtcagtgta atatttcttt ttgaactatc cagcttagta
     9481 ccgtacggga aagtgactgg tgtgcttatc tttgaaatgt tactttgggt ttcggttctt
     9541 taggttagta agaaagcact tgtcttctca tacaaaggaa aacctgagac gtatcgctta
     9601 cgaaagtagc aatgaaagaa aggtggtggt tttaatcgct accgcaaaaa cgatggggtc
     9661 gttttaatta acttctccta cgcaagcgtc taaacggacg ttggggtttt gctagtttct
     9721 ttagagaaaa ctagctaagt ctttaatgtt atcattagag atggcataaa tataatactt
     9781 gtgtctgctg ataagatcat tttaatttgg acgattagac ttgttgaact acaggttact
     9841 gaatcacttg cgctaatcaa catgggagat atgtacgatg aatcatttga caagtcgggc
     9901 ggtcctgctg acttgatgga cgattcttgg gtggaatcag tttcgtggaa agatctgttg
     9961 aagaagttac acagcataaa atttgcacta cagtctggta gagatgagat cactgggtta
    10021 ctagcggcac tgaatagaca gtgtccttat tcaccatatg agcagtttcc agataagaag
    10081 gtgtatttcc ttttagactc acgggctaac agtgctcttg gtgtgattca gaacgcttca
    10141 gcgttcaaga gacgagctga tgagaagaat gcagtggcgg gtgttacaaa tattcctgcg
    10201 aatccaaaca caacggttac gacgaaccaa gggagtacta ctactaccaa ggcgaacact
    10261 ggctcgactt tggaagaaga cttgtacact tattacaaat tcgatgatgc ctctacagct
    10321 ttccacaaat ctctaacttc gttagagaac atggagttga agagttatta ccgaaggaac
    10381 tttgagaaag tattcgggat taagtttggt ggagcagctg ctagttcatc tgcaccgcct
    10441 ccagcgagtg gaggtccgat acgtcctaat ccctagggat ttaaggacgt gaactctgtt
    10501 gagatctctg tgaaattcag agggtgggtg ataccatatt cactgatgcc attagcgaca
    10561 tctaaatagg gctaattgtg actaatttga gggaatttcc tttaccattg acgtcagtgt
    10621 cgttggtagc atttgagttt cggagcatct tgttctgggg tttcacacta tctttagaga
    10681 aagtgttaag ttaattaagt tatcttaatt aagagcataa ttatactgat ttgtctctcg
    10741 ttgatagagt ctatcattct gttactaaaa atttgacaac tcggtttgct gacctactgg
    10801 ttactgtatc acttacccga gttaacgagt ctagaaacgg actcttgcca gcaatgctgt
    10861 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt
    10921 gaaaaagtgg caccgagtcg gtgctcccgc tcccgtagct cagttggtta gagcgttggt
    10981 cttatgagcc gaaggtcgcg ggttcgagcc ccgccggaag caagtg
//
