LOCUS       sequence-458140-       10888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 10888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRD-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..10888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        1767..2146
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2147..2350
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2301..2321
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2347..2371
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2581..2599
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2581..2598
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2612..2631
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             6493..6528
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6538..6561
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             8221..8265
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             8266..8292
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(8432..8449)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8438..8549
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8667..8687)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8672..9001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8852..8987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8914..8933
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9049..9096
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9115..9714
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9115..9134)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9611..9631
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9844..9965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9881..9900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9935..9954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(10018..10809,10810..10878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10641..10660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10879..10888,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     1741 ctcatgtcca atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc
     1801 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     1861 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     1921 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     1981 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     2041 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     2101 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     2161 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     2221 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     2281 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     2341 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     2401 cctccataga agacaccggg accgatccag cctccgcggc cgggaacggt gcattggaac
     2461 gcggattccc cgtgccaaga gtgacgtaag taccgcctat agagtctata ggcccacccc
     2521 cttggcttcg ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg
     2581 atttaggtga cactatagaa taacatccac tttgcctttc tctccacagg tgtccactcc
     2641 caggtccaac tgcacctcgg ttctatcgaa aacgcgcctg ctagcgccac catgaagacc
     2701 atcattgccc tgagctacat cttctgcctg gtgttcgctg cggccgccga aacacctcct
     2761 agattcacca gaacacccgt ggaccagaca ggtgttagtg gcggagtggc cagcttcatc
     2821 tgtcaagcca caggcgaccc cagacctaag atcgtgtgga acaagaaagg caagaaggtg
     2881 tccaaccagc gcttcgaagt gatcgagttc gatgatggca gcggcagcgt gctgagaatc
     2941 cagcctctga gaacacccag agatgaggcc atctacgagt gcgtggccag caacaatgtg
     3001 ggcgagatca gcgtgtccac cagactgaca gtgctgagag aggatcagat ccccagaggc
     3061 ttccccacca tcgatatggg acctcagctg aaggtggtgg aaagaaccag aaccgccaca
     3121 atgctgtgtg ccgcctctgg caatcctgat cctgagatta cctggttcaa ggacttcctg
     3181 cctgtggaca cctccaacaa caacggccgg atcaagcagc tgagaagcga gtctatcggc
     3241 ggcactccta ttagaggcgc cctgcagatt gagcagagcg aggaaagcga ccagggcaaa
     3301 tatgagtgtg tggccaccaa tagcgccggc acaagatact ctgcccctgc caatctgtac
     3361 gtgcgcgaac tgagagaagt gcggagagtg cctcctcggt ttagcatccc tcctaccaac
     3421 cacgagatca tgcctggcgg ctccgtgaat atcacatgtg tggccgtggg cagccccatg
     3481 ccttatgtga aatggatgct gggcgccgag gatctgaccc ctgaggatga tatgcccatc
     3541 ggcagaaacg tgctggaact gaacgatgtg cggcagagcg ccaattacac ctgtgtggct
     3601 atgagcaccc tgggcgtgat cgaggccatt gctcagatca cagtgaaggc cctgcctaag
     3661 cctcctggca cacctgtggt tacagagagc acagccacca gcatcaccct gacctgggat
     3721 agcggaaatc ccgagcctgt gtcctactac atcatccagc acaagcccaa gaacagcgag
     3781 gaactgtaca aagaaatcga cggcgtggcc actaccagat acagtgtggc cggactgagc
     3841 ccctacagcg actacgagtt tagagtggtg gccgtgaaca acatcggcag gggtcctcca
     3901 tctgagcctg tgctgacaca gacatctgag caggccccat ctagcgcccc tagagatgtt
     3961 caggccagaa tgctgagcag caccaccatc ctggtgcagt ggaaagagcc tgaggaaccc
     4021 aacggccaga tccagggcta cagagtgtac tacacaatgg accccacaca gcacgtcaac
     4081 aactggatga agcacaacgt ggccgacagc cagatcacca caatcggcaa cctggtgcct
     4141 cagaaaacct actccgtgaa ggtgctggcc ttcacctcta tcggagatgg ccctctgagc
     4201 agcgacatcc aagtgatcac acagactggc gtgcccggcc agccactgaa tttcaaggcc
     4261 gagcctgaga gcgaaaccag catcctgctt agctggaccc ctcctagaag cgacacaatc
     4321 gccaactacg agctggtgta caaggatggc gagcacggcg aggaacagag aatcacaatc
     4381 gagcccggca ccagctacag actgcagggc ctgaagccta acagcctgta ctacttcaga
     4441 ctggccgcca gatctcctca aggcctggga gcctctacag ccgagatctc tgccagaacc
     4501 atgcagagca aacccagcgc tcctccacag gacatcagct gtacaagccc cagctctacc
     4561 tctatcctgg tgtcttggca gcctcctcca gtggaaaagc agaacggcat catcaccgag
     4621 tacagcatca agtacacagc cgtggacggc gaagatgaca agcctcacga gatcctgggc
     4681 atccccagcg ataccaccaa gtacctgctg gaacagctgg aaaagtggac cgagtaccgg
     4741 atcaccgtga cagcccacac agatgttgga cctggacctg agagcctgtc cgtgctgatc
     4801 agaacaaacg aggatgtgcc tagcggccct cctcggaaag tggaagtgga agccgtgaac
     4861 tccaccagcg tgaaagtgtc ttggagaagc cccgtgccta acaagcagca tggccagatc
     4921 agaggatacc aggtgcacta cgtgcggatg gaaaacggcg agcctaaggg ccagcctatg
     4981 ctgaaggatg tcatgctggc cgacgctcag tgggagtttg atgacaccac cgagcacgac
     5041 atgatcatca gcggactgca gcccgagaca agctactctc tgacagtgac cgcctacacc
     5101 acaaaaggcg acggcgctag aagcaagcct aagctggttt ctacaactgg cgccgtgcct
     5161 ggcaagccca gactggttat caatcacacc cagatgaaca cagccctgat ccagtggcac
     5221 ccacctgtgg atacctttgg acctctgcag ggatacagac tgaagttcgg ccggaaggac
     5281 atggaacccc tgaccacact ggaattcagc gagaaagagg accacttcac cgccaccgat
     5341 attcacaagg gcgccagcta cgtgttccgg ctgagcgcca gaaacaaagt cggcttcggc
     5401 gaagagatgg tcaaagagat cagcatcccc gaagaggtgc ccaccggctt tccacagaat
     5461 ctgcacagcg agggcaccac ctctacaagc gttcagctct cttggcaacc accagtgctg
     5521 gctgagcgga atggaatcat cacaaagtac accctgctgt accgggacat caacatccct
     5581 ctgctgccca tggaacaact gatcgtgccc gccgatacca caatgaccct gaccggactg
     5641 aagcctgata ccacctacga tgtgaaagtg cgggcccaca caagcaaagg ccctggacct
     5701 tatagcccca gcgtgcagtt tagaaccctg ccagttgacc aggtgttcgc caagaacttc
     5761 cacgtgaagg ccgtgatgaa gacctccgtg ctgctgtctt gggagatccc cgagaactac
     5821 aacagcgcca tgccattcaa gatcctgtac gacgacggca agatggtgga agaggtggac
     5881 ggcagagcca ctcagaaact gattgtgaat ctgaagcccg agaagtccta cagcttcgtg
     5941 ctgaccaaca gaggcaattc tgctggcggc ctgcagcaca gagtgacagc caaaacagct
     6001 cccgacgtgc tgaggaccaa gcctgccttt atcggcaaga ccaacctgga cggcatgatt
     6061 accgtgcagc tgcctgaagt gcccgccaac gagaacatca agggctacta tatcatcatc
     6121 gtgcccctga agaagtcccg gggcaagttc atcaagcctt gggagagccc tgacgagatg
     6181 gaactggacg agctgctgaa agagatctcc cggaagcgga gatccatcag atatggcaga
     6241 gaggtcgagc tgaagcccta tatcgccgct catttcgatg tgctgcccac cgagtttaca
     6301 ctgggcgacg ataagcacta cggcggcttc accaacaaac agctgcagag cggccaagag
     6361 tacgtgttct ttgtgctggc agtgatggaa cacgccgaga gcaagatgta cgccacctct
     6421 ccatacagcg accccgtggt gtctatggat ctggaccctc agcctatcac cgacgaagag
     6481 gaaggcgcgc cggaagatca agttgatcca agattgattg atggcaaggg cggatctctg
     6541 gaggtgctgt tccagggacc aggcggaagc ggcaatcccg gcggcgatct ggccccccag
     6601 atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc
     6661 caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga cgcctgcggt
     6721 atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa cccggacttc
     6781 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
     6841 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
     6901 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
     6961 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
     7021 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
     7081 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     7141 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     7201 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     7261 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     7321 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     7381 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     7441 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     7501 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     7561 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     7621 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     7681 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     7741 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     7801 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     7861 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     7921 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     7981 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     8041 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     8101 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     8161 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc gggtggcgga
     8221 ggactgaacg acatcttcga ggctcagaaa atcgaatggc acgaacatca ccatcaccat
     8281 caccatcacc attaaggccg gcctgtagtc actggcacaa cgcgttaccc ggtaagccaa
     8341 tcgggtatac acggtcgtca tactgcagac agggttcttc tactttgcaa gatagtcttg
     8401 agtagtaaaa taaatagata gagaaaatca gcctcgactg tgccttctag ttgccagcca
     8461 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     8521 ctttcctaat aaaatgagga aattgcatca caacactcaa ccctatctcg gtctattctt
     8581 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     8641 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     8701 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     8761 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     8821 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8881 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8941 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     9001 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     9061 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatgacc
     9121 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     9181 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     9241 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     9301 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     9361 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     9421 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     9481 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     9541 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagaca
     9601 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9661 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacacgtg
     9721 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     9781 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     9841 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9961 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatta
    10021 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10081 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10141 cgctgcgatg ataccgcgag aaccacgctc accggctccg gatttatcag caataaacca
    10201 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10261 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10321 tgttgccatc gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10381 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10441 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10501 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10561 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10621 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10681 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10741 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10801 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10861 gaaatgttga atactcatat tcttcctt
//
