LOCUS       sequence-458144-       10563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with mobile gRNA targeting SlCHL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 10563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pDL562
FEATURES             Location/Qualifiers
     source          1..10563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             456..473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      508..760
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    782..806
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2107..2736
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3165..4238
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4304..4498
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4734..4756)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4842..4982
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4897..4916
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4997..5014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5168..5756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5248..5267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6890..7684)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8109..8133
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        8662..9007
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     8950..8970
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 atgtcccgaa gacattaaac tacggttctt taagtagatc cgtgtctgaa gttttaggtt
       61 caatttaaac ctacgagatt gacattctcg actgatcttg attgatcggt aagtcttttg
      121 taatttaatt ttctttttga ttttatttta aattgttatc tgtttctgtg tatagactgt
      181 ttgagatcgg cgtttggccg actcattgtc ttaccatagg ggaacggact ttgtttgtgt
      241 tgttatttta tttgtatttt attaaaattc tcaacgatct gaaaaagcct cgcggctaag
      301 agattgttgg ggggtgagta agtactttta aagtgatgat ggttacaaag gcaaaagggg
      361 taaaacccct cgcctacgta agcgttatta cgcccgtctg tacttatatc agtacactga
      421 cgagtcccta aaggacgaaa cgggagaacg ctagccacca ccaccaccac cacgtgtgaa
      481 ttacaggtga ccagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta
      541 agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt
      601 aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt
      661 agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag
      721 gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattaaa ctatcagtgt
      781 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataatcgga
      841 tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac
      901 agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat agtgcagtcg
      961 gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta
     1021 cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca
     1081 taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag agcgccgccg
     1141 ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc aaccaacggg
     1201 ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc ggcaccaggc
     1261 gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac gttgtgacag
     1321 tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt gccgagcgca
     1381 tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac accaccacgc
     1441 cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag cgttccctaa
     1501 tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc
     1561 cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc gaccaggaag
     1621 gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg
     1681 cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt gccttccgtg
     1741 aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc caagaggaac
     1801 aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg aagagatcga
     1861 ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg
     1921 gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc cggccagctt
     1981 ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag
     2041 cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa tacgcaaggg
     2101 gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc
     2161 gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt agtcgattcc
     2221 gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt
     2281 gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc
     2341 gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat caaggcagcc
     2401 gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac cgccgacctg
     2461 gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc ggcctttgtc
     2521 gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg
     2581 tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc
     2641 gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg cgaggtccag
     2701 gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa gagaaaatga
     2761 gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa
     2821 cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag ttgccggcgg
     2881 aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt accgagctgc
     2941 tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat gagtagatga
     3001 attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac cgacgccgtg
     3061 gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg ggttgcctgc
     3121 cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac
     3181 catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag aagttgaagg
     3241 ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt gaatcgtggc
     3301 aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt
     3361 cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg atgctctatg
     3421 acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc
     3481 gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac gtagaggttt
     3541 ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg atggcggttt
     3601 cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag cccggccgcg
     3661 tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat ggcggaaagc
     3721 agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt gccatgcagc
     3781 gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa gccttgatta
     3841 gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag atcgagctag
     3901 ctgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg acggttcacc
     3961 ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg gcacgccgcg
     4021 ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc agtggcagcg
     4081 ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc
     4141 cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc atgcgctacc
     4201 gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag atgctagggc
     4261 aaattgccct agcaggggaa aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca
     4321 ttgggaaccc aaagccgtac attgggaacc ggaacccgta cattgggaac ccaaagccgt
     4381 acattgggaa ccggtcacac atgtaagtga ctgatataaa agagaaaaaa ggcgattttt
     4441 ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac
     4501 tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct
     4561 ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc tggccgctca aaaatggctg
     4621 gcctacggcc aggcaatcta ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc
     4681 ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     4741 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     4801 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     4861 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     4921 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     4981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     5041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     5101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     5161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     5221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     5281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     5341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5581 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     5641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaagggc
     5821 tgatgaatcc cctaatgatt tttatcaaaa tcattaagtt aaggtagata cacatcttgt
     5881 catatgatca aatggtttcg ccaaaaatca ataatcagac aacaaaatgt gcgaactcga
     5941 tattttacac gactctcttt accaattctg ccccgaatta cacttaaaac gactcaacag
     6001 cttaacgttg gcttgccacg ccttacttga ctgtaaaact ctcactctta ccgaacttgg
     6061 ccgtaacctg ccaaccaaag cgagaacaaa acataacatc aaacgaatcg accgattgtt
     6121 aggtaatcgt cacctccaca aagagcgact cgctgtatac cgttggcatg ctagctttat
     6181 ctgttcgggc aatacgatgc ccattgtact tgttgactgg tctgatatcc gtgagcaaaa
     6241 acggcttatg gtattgcgag cttcagtcgc actacacggt cgttctgtta ctctttatga
     6301 gaaagcgttc ccgctttcag agcaatgttc aaagaaagct catgaccaat ttctagccga
     6361 ccttgcgagc attctaccga gtaacaccac accgctcatt gtcagtgatg ctggctttaa
     6421 agtgccatgg attctagcaa ctaacttacc tgttgaaatt cgaacaccca aacaacttgt
     6481 taatatctat tcgaagcgaa tgcagattga agaaaccttc cgagacttga aaagtcctgc
     6541 ctacggacta ggcctacgcc atagccgaac gagcagctca gagcgttttg atatcatgct
     6601 gctaatcgcc ctgatgcttc aactaacatg ttggcttgcg ggcgttcatg ctcagaaaca
     6661 aggttgggac aagcacttcc aggctaacac agtcagaaat cgaaacgtac tctcaacagt
     6721 tcgcttaggc atggaagttt tgcggcattc tggctacaca ataacaaggg aagactcact
     6781 cgtggctgca accctgctta ctcaaaatct attcacacat ggttacgttt tggggaaatt
     6841 atgaggggat ctctcagcgt taagggattt tggtcatgca ttctaggtac taaaacaatt
     6901 catccagtaa aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa
     6961 aaaatagctc gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg
     7021 caatgtcata ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt
     7081 tgccatcttt cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct
     7141 cttcgggctt ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt
     7201 cttcttccca gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt
     7261 cggctaagcg gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa
     7321 agagcctgat gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat
     7381 actcttccga gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat
     7441 gccgttcaaa gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt
     7501 ccttttcccg ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat
     7561 ataggttttc attttctccc accagcttat ataccttagc aggagacatt ccttccgtat
     7621 cttttacgca gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag
     7681 ccatttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta
     7741 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac
     7801 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt
     7861 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct
     7921 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc
     7981 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact
     8041 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg
     8101 ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac
     8161 acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcctag gccaccatgt
     8221 tgggcccggc gcgccaagct tgcatgcctg caggtcaaca tggtggagca cgacactctc
     8281 gtctactcca agaatatcaa agatacagtc tcagaagacc agagggctat tgagactttt
     8341 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
     8401 atcgaaagga cagtagaaaa ggaagatggc ttctacaaat gccatcattg cgataaagga
     8461 aaggctatcg ttcaagatgc ctctaccgac agtggtccca aagatggacc cccacccacg
     8521 aggaacatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
     8581 gatggtcaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
     8641 ctcagaagac cagagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
     8701 cggattccat tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaagatgg
     8761 cttctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctaccga
     8821 cagtggtccc aaagatggac ccccacccac gaggaacatc gtggaaaaag aagacgttcc
     8881 aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc
     8941 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     9001 gaggataaaa cattgcacct atggtgttgc cctggctggg gtatgtcagt gatcgcagta
     9061 gaatgtacta attgacaagt tggagaatac ggtagaacgt ccttatccaa cacagccttt
     9121 atccctctcc ctgacgaggt ttttgtcagt gtaatatttc tttttgaact atccagctta
     9181 gtaccgtacg ggaaagtgac tggtgtgctt atctttgaaa tgttactttg ggtttcggtt
     9241 ctttaggtta gtaagaaagc acttgtcttc tcatacaaag gaaaacctga gacgtatcgc
     9301 ttacgaaagt agcaatgaaa gaaaggtggt ggttttaatc gctaccgcaa aaacgatggg
     9361 gtcgttttaa ttaacttctc ctacgcaagc gtctaaacgg acgttggggt tttgctagtt
     9421 tctttagaga aaactagcta agtctttaat gttatcatta gagatggcat aaatataata
     9481 cttgtgtctg ctgataagat cattttaatt tggacgatta gacttgttga actacaggtt
     9541 actgaatcac ttgcgctaat caacatggga gatatgtacg atgaatcatt tgacaagtcg
     9601 ggcggtcctg ctgacttgat ggacgattct tgggtggaat cagtttcgtg gaaagatctg
     9661 ttgaagaagt tacacagcat aaaatttgca ctacagtctg gtagagatga gatcactggg
     9721 ttactagcgg cactgaatag acagtgtcct tattcaccat atgagcagtt tccagataag
     9781 aaggtgtatt tccttttaga ctcacgggct aacagtgctc ttggtgtgat tcagaacgct
     9841 tcagcgttca agagacgagc tgatgagaag aatgcagtgg cgggtgttac aaatattcct
     9901 gcgaatccaa acacaacggt tacgacgaac caagggagta ctactactac caaggcgaac
     9961 actggctcga ctttggaaga agacttgtac acttattaca aattcgatga tgcctctaca
    10021 gctttccaca aatctctaac ttcgttagag aacatggagt tgaagagtta ttaccgaagg
    10081 aactttgaga aagtattcgg gattaagttt ggtggagcag ctgctagttc atctgcaccg
    10141 cctccagcga gtggaggtcc gatacgtcct aatccctagg agcatcttgt tctggggttt
    10201 cacactatct ttagagaaag tgttaagtta attaagttat cttaattaag agcataatta
    10261 tactgatttg tctctcgttg atagagtcta tcattctgtt actaaaaatt tgacaactcg
    10321 gtttgctgac ctactggtta ctgtatcact tacccgagtt aacgagtcta gtactcctgc
    10381 tcgatttatc cttatgttta agagctatgc tggaaacagc atagcaagtt taaataaggc
    10441 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctcccgctcc cgtagctcag
    10501 ttggttagag cgttggtctt atgagccgaa ggtcgcgggt tcgagccccg ccggaagcag
    10561 gat
//
