LOCUS       sequence-458177-       10215 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Liu D, Ellison EE, Myers EA, Donahue LI, Xuan S, Swanson R, Qi S, 
            Prichard LE, Starker CG, Voytas DF
  TITLE     Heritable gene editing in tomato through viral delivery of 
            isopentenyl transferase and single-guide RNAs to latent axillary 
            meristematic cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Sep 24;121(39):e2406486121. doi: 
            10.1073/pnas.2406486121. Epub 2024 Sep 16.
  PUBMED    39284063
REFERENCE   2  (bases 1 to 10215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-09-24"; volume: "121(39):e2406486121. 
            doi"; pages: " 10.1073/pnas.2406486121. Epub 2024 Sep 16"
COMMENT     Sequence Label: pEE844
FEATURES             Location/Qualifiers
     source          1..10215
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             335..640
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     519..538
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             1111..1128
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1163..1415
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1437..1461
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2762..3391
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3820..4893
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4959..5153
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5389..5411)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5497..5637
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5552..5571
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5652..5669)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5823..6411)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5903..5922)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7545..8339)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8764..8788
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        9317..9662
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     9605..9625
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 caacggcggc aggtgaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
       61 taagaatata tactgatatg tatacccgaa gtatgtcaaa aggaggtatg ctatgaagca
      121 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
      181 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
      241 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
      301 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
      361 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
      421 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
      481 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
      541 cagtgtgccg gtttccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
      601 catcaaaaac gccattaacc tgatgttctg gggaatataa cacctgcgct cggatatgtc
      661 ccgaagacat taaactacgg ttctttaagt agatccgtgt ctgaagtttt aggttcaatt
      721 taaacctacg agattgacat tctcgactga tcttgattga tcggtaagtc ttttgtaatt
      781 taattttctt tttgatttta ttttaaattg ttatctgttt ctgtgtatag actgtttgag
      841 atcggcgttt ggccgactca ttgtcttacc ataggggaac ggactttgtt tgtgttgtta
      901 ttttatttgt attttattaa aattctcaac gatctgaaaa agcctcgcgg ctaagagatt
      961 gttggggggt gagtaagtac ttttaaagtg atgatggtta caaaggcaaa aggggtaaaa
     1021 cccctcgcct acgtaagcgt tattacgccc gtctgtactt atatcagtac actgacgagt
     1081 ccctaaagga cgaaacggga gaacgctagc caccaccacc accaccacgt gtgaattaca
     1141 ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     1201 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     1261 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     1321 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     1381 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttaaactatc agtgtttgac
     1441 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     1501 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     1561 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     1621 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     1681 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     1741 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     1801 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     1861 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     1921 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     1981 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     2041 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     2101 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     2161 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     2221 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     2281 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     2341 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     2401 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     2461 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     2521 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     2581 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     2641 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     2701 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     2761 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     2821 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     2881 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     2941 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     3001 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     3061 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     3121 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     3181 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     3241 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     3301 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     3361 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     3421 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     3481 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     3541 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     3601 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     3661 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     3721 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
     3781 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
     3841 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     3901 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     3961 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     4021 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     4081 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     4141 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     4201 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     4261 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     4321 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     4381 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     4441 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     4501 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     4561 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     4621 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     4681 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     4741 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     4801 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     4861 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     4921 gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac gtacattggg
     4981 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     5041 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     5101 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     5161 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     5221 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     5281 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     5341 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5401 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5461 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
     5521 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
     5581 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
     5641 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5701 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5761 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     5821 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     5881 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     5941 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6001 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6061 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6121 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6181 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6241 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6301 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6361 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6421 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggctgatg
     6481 aatcccctaa tgatttttat caaaatcatt aagttaaggt agatacacat cttgtcatat
     6541 gatcaaatgg tttcgccaaa aatcaataat cagacaacaa aatgtgcgaa ctcgatattt
     6601 tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa
     6661 cgttggcttg ccacgcctta cttgactgta aaactctcac tcttaccgaa cttggccgta
     6721 acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta
     6781 atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt
     6841 cgggcaatac gatgcccatt gtacttgttg actggtctga tatccgtgag caaaaacggc
     6901 ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag
     6961 cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg
     7021 cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc
     7081 catggattct agcaactaac ttacctgttg aaattcgaac acccaaacaa cttgttaata
     7141 tctattcgaa gcgaatgcag attgaagaaa ccttccgaga cttgaaaagt cctgcctacg
     7201 gactaggcct acgccatagc cgaacgagca gctcagagcg ttttgatatc atgctgctaa
     7261 tcgccctgat gcttcaacta acatgttggc ttgcgggcgt tcatgctcag aaacaaggtt
     7321 gggacaagca cttccaggct aacacagtca gaaatcgaaa cgtactctca acagttcgct
     7381 taggcatgga agttttgcgg cattctggct acacaataac aagggaagac tcactcgtgg
     7441 ctgcaaccct gcttactcaa aatctattca cacatggtta cgttttgggg aaattatgag
     7501 gggatctctc agcgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc
     7561 agtaaaatat aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat
     7621 agctcgacat actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg
     7681 tcataccact tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca
     7741 tctttcacaa agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg
     7801 ggcttttccg tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct
     7861 tcccagtttt cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct
     7921 aagcggctgt ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc
     7981 ctgatgcact ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct
     8041 tccgagcaaa ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt
     8101 tcaaagtgca ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt
     8161 tcccgttcca catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg
     8221 ttttcatttt ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt
     8281 acgcagcggt atttttcgat cagttttttc aattccggtg atattctcat tttagccatt
     8341 tattatttcc ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca
     8401 agacgaactc caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt
     8461 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
     8521 cgcggtgatc acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg
     8581 agatcatccg tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa
     8641 catgagcaaa gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg
     8701 gctgcctgta tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc
     8761 tggtggcagg atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt
     8821 gcggacgttt ttaatgtact gaattaacgc cgaattaatt cctaggccac catgttgggc
     8881 ccggcgcgcc aagcttgcat gcctgcaggt caacatggtg gagcacgaca ctctcgtcta
     8941 ctccaagaat atcaaagata cagtctcaga agaccagagg gctattgaga cttttcaaca
     9001 aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga
     9061 aaggacagta gaaaaggaag atggcttcta caaatgccat cattgcgata aaggaaaggc
     9121 tatcgttcaa gatgcctcta ccgacagtgg tcccaaagat ggacccccac ccacgaggaa
     9181 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatgg
     9241 tcaacatggt ggagcacgac actctcgtct actccaagaa tatcaaagat acagtctcag
     9301 aagaccagag ggctattgag acttttcaac aaagggtaat atcgggaaac ctcctcggat
     9361 tccattgccc agctatctgt cacttcatcg aaaggacagt agaaaaggaa gatggcttct
     9421 acaaatgcca tcattgcgat aaaggaaagg ctatcgttca agatgcctct accgacagtg
     9481 gtcccaaaga tggaccccca cccacgagga acatcgtgga aaaagaagac gttccaacca
     9541 cgtcttcaaa gcaagtggat tgatgtgata tctccactga cgtaagggat gacgcacaat
     9601 cccactatcc ttcgcaagac ccttcctcta tataaggaag ttcatttcat ttggagagga
     9661 taaaacattg cacctatggt gttgccctgg ctggggtatg tcagtgatcg cagtagaatg
     9721 tactaattga caagttggag aatacggtag aacgtcctta tccaacacag cctttatccc
     9781 tctccctgac gaggtttttg tcagtgtaat atttcttttt gaactatcca gcttagtacc
     9841 gtacgggaaa gtgactggtg tgcttatctt tgaaatgtta ctttgggttt cggttcttta
     9901 ggttagtaag aaagcacttg tcttctcata caaaggaaaa cctgagacgt atcgcttacg
     9961 aaagtagcaa tgaaagaaag gtggtggttt taatcgctac cgcaaaaacg atggggtcgt
    10021 tttaattaac ttctcctacg caagcgtcta aacggacgtt ggggttttgc tagtttcttt
    10081 agagaaaact agctaagtct ttaatgttat cattagagat ggcataaata taatacttgt
    10141 gtctgctgat aagatcattt taatttggac gattagactt gttgaactac aggttactga
    10201 atcacttgcg ctaat
//
