LOCUS       sequence-458196-        9996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible Tol2 destination vector containing the hsp70 
            promoter driving Cre and TagBFP in endothelial cells followed by 2 
            cHS4 insulator cores. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)
FEATURES             Location/Qualifiers
     source          1..9996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(667..678,679..841)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    1245..1368
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(1409..1714)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1511..1530)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(2034..2714)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     2629..2648
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2768..2774,2775..2792,2793..2798))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2823..2946)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2954..2970)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2990..3071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    3595..3729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     3686..3705
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3751..3771)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3796..4494)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     intron          5320..5452
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(5687..5707)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      5710..5719
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(7584..7771,7772..7783)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7808..7828)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7808..7826)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7847..7863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7847..7863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7860..7882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7871..7887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7895..7901,7902..7919,7920..7925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7940..7961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8078..8095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8249..8837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8329..8348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9008..9799,9800..9868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9631..9650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9869..9973)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctt
      661 aattaacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
      721 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
      781 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
      841 aagaaaacta gagattcttg tttaagctat ttaatctcaa aaaacattaa atgaaatgca
      901 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      961 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
     1021 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
     1081 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
     1141 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1201 gatcctaggg ggcccgttta aacgccatga ttacgccaag ctatcaactt tgtatagaaa
     1261 agttgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga ttttgcataa
     1321 aaaacagact acataatact gtaaaacaca acatatccag tcactatggt cgacctgcag
     1381 actggctgtg tataagggag cctgacattt atattcccca gaacatcagg ttaatggcgt
     1441 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggagaccg
     1501 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
     1561 ggtaaagttc acgggggact ttatctgaca gcagacgtgc actggccagg gggatcacca
     1621 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
     1681 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcggca
     1741 aaggagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
     1801 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccgaaccg
     1861 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
     1921 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
     1981 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttttagtaa
     2041 gccggatcct ctagattacg ccccgcctgc cactcatcgc agtactgttg taattcatta
     2101 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
     2161 atcagcacct tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag
     2221 ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag
     2281 acgaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac
     2341 gccacatctt gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag
     2401 agcgatgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc
     2461 catatcacca gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg
     2521 cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt
     2581 aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga
     2641 aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg
     2701 atttttttct ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa
     2761 atccacacat tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc
     2821 gccatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     2881 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcaact ttattataca
     2941 tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatca acttgtttat
     3001 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3061 tttttcactg cgatatcaat cgggacagcc cccccccaaa gcccccaggg atgtaattac
     3121 gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg gtccggcgct
     3181 ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg aaggtggcac
     3241 gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca cctgggggga
     3301 tacggggaaa aagctttagg ctgagtgcac ggggacagcc cccccccaaa gcccccaggg
     3361 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     3421 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     3481 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     3541 cctgggggga tacggggaaa aagctttagg ctgatacgta gtagatgcgg ccgcaacttg
     3601 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     3661 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     3721 gtctggatcg cgatcgcgtc tagattatca tacctttctc ttcttttttg gaccaccagc
     3781 accacctcct cgagcattaa gcttgtgccc cagtttgcta gggaggtcgc agtatctggc
     3841 cactgccacc tcgtgctgct cgacgtaggt ctcgttgttg gcctccttga ttctttccag
     3901 tctgtagtcc acatagtaga cgccaggcat cttgaggttc ttagcgggtt tcttggatct
     3961 atatgtggtc ttggcgtttg cgatcagatg gctcccgccc acgagcttca gggccatgtc
     4021 gtttctgcct tccaggccgc cgtcagcggg gtacagcgtc tcggtgaagg cctcccagcc
     4081 gagtgttttc ttctgcatca cagggccgtt ggatgtgaag ttcacccctc tgatcttgac
     4141 gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc
     4201 gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact gcttgaagaa
     4261 gtcggggatg ccctgggtgt ggttgatgaa ggtcttgctg ccgtagagga agctagtagc
     4321 caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg
     4381 ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtgat ggttgtccac
     4441 ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctcgc tcatggtgga
     4501 tccggccccg ccggctccgc cgctagcgtc tccatcctcc agcagtctca ccatagctcc
     4561 tgtctcagag tccaggtttc tgatgtagtt catcacaatg ttcacatttg tccatcctcc
     4621 agcctgcatg atctcaggga tgctcactcc agctctagcc atgtctctag cagctcccac
     4681 tctagcagag tgtccagacc aagccaggta tctctgtcca gagtcatcct tagctccata
     4741 gatcagtctg tgtgtagcct caaagattcc ctccagagcc cttgtgctca gctggcttgt
     4801 agcagaagga gcagccactc cattctttct cactctacag aacaggtagt tgttagggtc
     4861 atcagccact ccagacacag agatccatct ctccaccagc tttgtcactc ccaggctcag
     4921 agccttctcc actccagctg tgctcaccag tgtctttgtt cttccaatgt ggatcagcat
     4981 tcttcctcca tctgttctgc tgatgtcctt cactctgatt ctagcaatct cagcaattct
     5041 cagcagtgtg ttgtaagcaa ttcccaggaa agccaggttt ctgatgtcct gacatctgtc
     5101 agagttctcc atcaggcttc tcacctggtc aaagtctgtt ctctcaaaag ccagagcctg
     5161 cttagctctc tctccagcat ccacattctc ctttctgatt cttctcatca ccaggctcac
     5221 agcattgctg tcagaaggtc taggcagtcc agaccttctg tgcagcatgt tcagctgtcc
     5281 caggtgctgc tggattgtct tcacagccag tcctctagcc tgtggagaga aaggcaaagt
     5341 ggatgtcagt aagaccaata ggtgcctatc agaaacgcaa gagtcttctc tgtctcgaca
     5401 agcccagttt ctattggtct ccttaaacct gtcttgtaac cttgatactt acctggaggt
     5461 acagcaggta gtctctcaca tcctcaggct cagcagggaa ccactttctg ttgttcagct
     5521 tacaccaagc agcccagctc ctgcagacag acagcagcat cttccatgtg tgctcagaga
     5581 aagcctgtct gtctctgaac atgtccatca ggttctttct cacctcatca gatgtagcat
     5641 ccacaggcag agcaggtaga ttttggtgga cggttagaag attggagact ttcctcttct
     5701 tcttgggcac catggtggcc atatgcggca cgcgtcagga aaaaaaaaaa caattagaat
     5761 taattttata tttatacaat ttatcggtgc aattgtttca ttatgaaagg atttttctag
     5821 tcaacaagtg aaattctctt aataaccaag ttattattag ttgtacaagt aaaacgctaa
     5881 ttttcttttg tagacaacat taacacaaat tcatttagga catttgctag tatttatggt
     5941 tattatgctc atataaaata atgtcagcaa cataaagtaa atgcccgtct gttcattgct
     6001 ttttttaaaa tccttattgt gaaaacggga aatataattt tctagtagac ctaaaataag
     6061 atgaacatag gcagtccaca tgtaaaaaga ttacatatac aaagttgatt ttttgacatt
     6121 aagtacatat aatctctata taattctgat tagccaatag agtacaatat attgagactt
     6181 accttacagt agattcttaa atagagcgaa taaacacctc gtcggggaaa agtcctgtca
     6241 ggctgctctt ttcttctttg tcaagtcgct tctcttcggt tgagtttctc tgcggtctct
     6301 ctttcgccct ttaaatacag cagagcgcgc acccgttcag actctacact gttctggaag
     6361 cctccagtga attcgaccaa tcaaaacaca gcaggcagcc gccgtcactt cttcagctgt
     6421 cattgagagg agccgctgag aggaatgtag tggagatttc cagagtttta ccgtgaaatt
     6481 acgcgaaaca gcggtcaaaa atacaaagag atttaaaatg ttttttttat ttgagtgttt
     6541 tattgtactt attgctgaaa tatgtgcggt tgtgtgatgt gttgtttttc cagttgcaat
     6601 gaatgaacaa actaaaatga taaaatgttt gaacgattca gtaaaaaaaa atgcaataaa
     6661 aaaatcttgc tgaaatgttt tacacagtag caaaatcggg cctgtggtgt tttcagctac
     6721 atagaaggca aacctgtttt atataagcta atagctttgg tcagatttgt ttaacgtttc
     6781 aacaaaacaa atggatgttt actgtactat attgattggt taaaaccaat actttattct
     6841 gggattgcag gtcttaacta tttttgtagt gttaaaaaca acttgctaac ttgagaggtc
     6901 atgtttaccc tcagagaaat aggcacaagt ctctttgttt aatattggag atcatcctcc
     6961 ttgttcagtc gtggtctgta tttaatttta atgtatatga ttgccgtaaa agtgttagaa
     7021 agtttaccct ggtgctatga aatcaatctg ctgcttttga cccagttagt gtgtctttaa
     7081 tctaacttaa tgagacccca gtgtggtcaa caatcaatag aaacaaatgt gttgttttat
     7141 aactgttaac tcctattatt ttttatcttc tgtagcatat agacaaaaca tgctcgttct
     7201 aatagtttaa taaaattaat ttgatttttg gaaataacca agcgacaccc ctgaactagt
     7261 atcgatgcga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
     7321 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
     7381 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
     7441 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
     7501 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
     7561 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
     7621 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
     7681 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
     7741 gtacagtaat caagtaaaat tactcaagta ctttacacct ctgagatctg agctccagct
     7801 tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc
     7861 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     7921 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     7981 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8041 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     8101 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     8161 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     8221 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     8281 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8341 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8401 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8461 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8521 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8581 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8641 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     8701 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8761 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8821 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8881 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8941 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     9001 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     9061 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     9121 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     9181 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     9241 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     9301 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     9361 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     9421 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     9481 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     9541 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     9601 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     9661 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     9721 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     9781 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     9841 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     9901 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     9961 taggggttcc gcgcacattt ccccgaaaag tgccac
//
