LOCUS       sequence-458197-       11154 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing ISCba controled by PlacM promoter, guide RNA controled 
            byPJ23119 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11154)
  AUTHORS   Wang X, Yang S, Qian F, Dong F, Zhou X, Yin M, Zhang Y, Huang Z, 
            Jiang Y, Yang S
  TITLE     RAGATH-Associated DNA Nuclease Assisted DNA Insertion in 
            Corynebacterium glutamicum.
  JOURNAL   ACS Synth Biol. 2025 May 8. doi: 10.1021/acssynbio.5c00022.
  PUBMED    40338137
REFERENCE   2  (bases 1 to 11154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 May 8. doi: 10.1021/acssynbio.5c00022."
COMMENT     Sequence Label: pISCba-reRNA
FEATURES             Location/Qualifiers
     source          1..11154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3672..4466
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3726..3745)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4336..4355
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             5726..5743
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      complement(5790..5817)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5909..5994)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     ncRNA           complement(5999..6066)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             complement(10137..11087)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(11135..11154,1..204)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
       61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt ttttttttgt
      121 ttatattcaa gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat
      181 agatcccagc cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat
      241 gtcgcaaacg ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag
      301 caccctcgca agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
      361 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
      421 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
      481 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
      541 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
      601 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
      661 aggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      721 aaaaggatct tcacctagat cctttacgct ccgggacgcc aacaacaaga cccatcatag
      781 tttgcccccg cgacattgac cataaattca tcgcacaaaa tatcgaacgg ggtttatgcc
      841 gcttttagtg ggtgcgaaga atagtctgct cattacccgc gaacaccgcc gcattcagat
      901 cacgcttagt agcgtcccca tgagtaggca gaaccgcgtc caagtccaca tcatccataa
      961 cgatcatgca cggggtggaa tccacaccca gacttgccag cacctcatta gcgacacgtt
     1021 gcgcagcggc cacgtcctta gccttatcca cgcaatcgag aacgtactgc ctaaccgcga
     1081 aatcagactg aatcagtttc caatcatcgg gcttcaccaa agcaacagca acgcgggttg
     1141 attcgacccg ttccggtgct tccagaccgg cgagcttgta cagttcttct tccatttcac
     1201 gacgtacatc agcgtctatg taatcaatgc ccaaagcacg cttagcccca cgtgaccagg
     1261 acgaacgcag gtttttagaa ccaacctcat actcacgcca ccgagccacc aaaacagcgt
     1321 ccatatcctc gccggcgtcg ctttgatcgg ccaacatatc caacatctga aacggcgtgt
     1381 acgacccctt agacgcggtt ttagtagcgg agccagtcag ttcctgagac atgcccttag
     1441 cgaggtaggt tgccattttc gcagcgtctc caccccaggt agacacctga tcaagtttga
     1501 ccccgtgctc acgcagtggc gcgtccatac cggccttaac cacaccagca gaccagcggg
     1561 aaaacatgga atcctcaaac gccttgagtt catcgtcaga cagtggacga tccaagaaca
     1621 acagcatgtt gcggtgcaag tgccaaccgt tcgcccaaga gtctgtgacc tcatagtcac
     1681 tataggtgtg ctccaccccg taccgtgcac gttctttctt ccactgagat gttttcacca
     1741 tcgaagagta cgcagtctta atacccgctt caacctgcgc aaatgactgt gagcggttgt
     1801 gtcgaacagt gcccacaaac atcatgagcg cgccacccgc cgccaagtga ttcttagtag
     1861 caatagccag ctcaatgcgg cgttcgccca tgacttccaa ttcagccaga gatgaccccc
     1921 agcgagagtg agagttttgc agaccctcaa actgcgaagc accgttagac gaccaggaca
     1981 ccgcaacagc ttcgtccctg cgccacctat ggcaccccgc cagagcctta ctattggtga
     2041 tcttgtacat gacgttttgc ctacgccacg ccctagcgcg agtgacctta gaaccctcat
     2101 tgacctgcgg ttccttagag gtgttcactt ctatttcagt gttacctaga cccgatgttg
     2161 tgcggggttg cgcagtgcga gtttgtgcgg gtgttgtgcc cgttgtctta gctagtgcta
     2221 tggttgtcaa ttgaaacccc ttcgggttat gtggcccccg tgcatatgag ttggtagctc
     2281 gcacgggggt ttgtcttgtc tagggactat taatttttag tggtgtttgg tggccgccta
     2341 gcttggctat gcgtgccagc ttacccgtac tcaatgttaa agatttgcat cgacatggga
     2401 gggttacgtg tccgatacct agggggggta tccgcgacta ggtgccccgg tgctcactgt
     2461 ctgtaccggc ggggcaagcc ccacaccccg catggacagg gtggctccgc cccctgcacc
     2521 cccagcaatc tgcatgtaca tgttttacac attagcacga catgactgca tgtgcatgca
     2581 ctgcatgcag actaggtaaa tatgagtatg tacgactagt aacaggagca ctgcacataa
     2641 tgaatgagtt gcaggacaat gtttgctacg catgcgcatg acatatcgca ggaaagctac
     2701 tagagtctta aagcatggca accaaggcac agctagaaca gcaactacaa gaagctcaac
     2761 aggcactaca ggcgcagcaa gcgcaggcac aagccaccat cgaagcacta gaagcgcagg
     2821 caaaggctaa gcccgtcgtg gtcaccgcac gcgttccttt ggcactacgt gaggacatga
     2881 agcgcgcagg catgcagaac ggtgaaaacc tccaagagtt catgatcgcc gcgtttaccg
     2941 agcggctaga aaagctcacc accaccgaca acgaggaaaa caatgtctaa cccactagtt
     3001 ctctttgccc accgtgaccc ggtaaatgac gtgacgttcg agtgcattga gcacgccacc
     3061 tacgacacac tttcacacgc taaagaccag atcaccgccc aaatgcaagc cctagacgaa
     3121 gaagccgccc tactgcccta atgggtgttt catgggtgtt tccctagtgt ttcatggtgt
     3181 tttcacctaa gctagggaat tgcgcgagaa gtctcgcaaa aatcagcaac ccccggaacc
     3241 acacagttca cgggggttct tctatgccag aaatcagaaa ggggaaccag tgaacgaccc
     3301 cgaatggctg gatgatcctc cttgggccac gcccacccgg cgatcatcga ggcggtcacc
     3361 ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca
     3421 gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca
     3481 gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat
     3541 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     3601 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag
     3661 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3721 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3781 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3841 tgaatgaact ccaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3901 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3961 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     4021 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     4081 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     4141 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     4201 ggatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     4261 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     4321 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     4381 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     4441 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgcggaat catgacctga
     4501 gctgtttaca attaatcatc gtgtggtacc atgtgtggaa ttggaaagga cttgaacgat
     4561 gaggaaattg ctaaagagct tcaagacgga aataaatccg acagaggagc agaaaaccaa
     4621 gattcgtaaa acaataggaa cttgcagatt tatttataac ttctatttgg ctcataacaa
     4681 aaagctccac gaagatggga aaaaatttat gagcagcaat aaatttagaa tctggcttaa
     4741 taacgaatat cttccacaac atccggagta tttgtggatc aaggaagcat attcaaaggc
     4801 tgtaacacag tcagtaaata acggacagac cgcatttaca agatttttca atcacgaaag
     4861 tgcttttcct aatttcaaaa agaaaggcaa atctgatgta aagatgtatt ttgtaaaaaa
     4921 taatccgaag gattgccatt gtgaaaggca tagaattaaa attccatcac ttggctgggt
     4981 tcgtatcaag gaaaaaggat atataccaac taccaaagat gggtacgtga ttaaaagcgg
     5041 ttcggtttca ataaaggctg acaggtatta tgtttcagtt cttgtggagg tctctgataa
     5101 caaaacgatc aatcatttcg acgaagggat tggcatagac ctcggactaa aagattttgc
     5161 cattgtatca aacggcagaa catatcaaaa cattaacaaa tcagcaaaac ttaaaaaact
     5221 tgagagacaa cttattcgag aacaaaggtg tctttcccgt aaatacgaaa atttaaagaa
     5281 aggagacgcc acccaaagag caaatataca aaaacaaaag ctcaaagtac aaaaacttca
     5341 ccataggata gataatatcc gtaccgatta catcaacaag acaatcgcag agatggtaaa
     5401 aatcaagcca tcttacataa cgattgaaga cttaaatgta aaaggcatga tgaagaaccg
     5461 gcatctctca aaagcggttg cttcgcagaa gttctatgag tttagagcga agctgctgac
     5521 taaatgcaat aaaagtggaa ttgagcttcg agtagtagac agatggtatc catcatctaa
     5581 gatctgccac tgctgcggat ccatcaaaaa agatttgaaa ctttcagata ggattttcaa
     5641 atgcagatgc ggttatatcg aagacagaga tttcaatgct tcacttaatc tgagagatgc
     5701 agaaacttac agcattgcaa gcagccatca ccatcatcac cactaagtcg acctgcaggc
     5761 atgcaagctt aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
     5821 taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
     5881 tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
     5941 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttaaatgt
     6001 cccattcgcc atgccgaagc atgttgccca gccggcgcca gcgaggaggc tgggaccatg
     6061 ccggccttcc actatttctt ctggcactgc cactcaaaat gtgattaaat atacccacat
     6121 cgagataatg taagttctca cgaacattac ttactgcttc attgtgtatg tttttgattg
     6181 accgtagtca tatctactca caagttcatg tacactccac agtcgtaaat tcccgaaata
     6241 gccttcggta catacttaca tttgcgtttt aaggatccat tatacctagg actgagctag
     6301 ctgtcaacta attgtttctg ccgcgggcaa ctaccttcca ggtacggaaa gtgccatcgg
     6361 cttcccgaac gtagacctca tccaccaaat taatcacgtt gcaggcatgg ttgggcacga
     6421 tgttgagctg ctccccgatt actggaagta gcactttatc tggccagaaa atggttgcgt
     6481 gatgctccga caaagcagag attcgggctt caggattccc cagaacaaaa ccattgccat
     6541 caatccatgc tggtttatca gtgctgagga ttttggatcc cgcatccaaa atgatccgac
     6601 gatctgacac atttcggctg accacagtag acagcaccgt cattgccacc tgcttctcag
     6661 tgcatgctcc cgaggtgatc tgctgggaat cgttaaacac atacacgcct ggtcgcatct
     6721 catcgattgc gtctgtaaac tgcgcagacg gcgaggaacc gccagaagtc aggccgccag
     6781 caagtcgctg gacgctgttg tttagagcct gaagctcatc agctgctgcc tgctcaccat
     6841 ttcccgggcc ataagaatgc ccaggaaaag taaacactcc tgcatacctg ctgcccagcg
     6901 cctcgcggat ctgactcaat tctgaagcag tcgccgtgac tccacttcta cgatgtcccg
     6961 aatccacttc aatcagagcc ttgatatctt cccgcaaacc cgccgtcgcc tgtgccatct
     7021 ctaccgaatc cacgccaatg gaaatttctc cggggatcgc gttcaggcgt tgcactgcat
     7081 gatcggttag atacagcgga tatgcaataa agatgtccgt aaaacctgcg ccggcaaaaa
     7141 tttccgcctc gccaatggtt gcgcaggtga tccctcgggc accggcgtcg acctgcatct
     7201 gcgcaatttc aatgattttg tgcgttttca catgcggacg cagggcaatc tcatgggcac
     7261 cggcgtgagc tgccatcctg gaaatgttgg cagttaagcg ctcgcggtca atgagaacag
     7321 caggtgtatc aatcatcatg gagtaggaat attccttatg ttcgaacggg ccagatcgat
     7381 catcgctcct actcctccac caaagacggt tcgggtggcc gccttgctga atcccatgac
     7441 ctgttcccac aatgtcctcc ccgggttgat attagatttc ataaatatac taaaaatctt
     7501 gagagttttt ccgttgaaaa ctaaaaagct gggaaggtga atcgaatttc ggggctttaa
     7561 agcaaaaatg aacagcttgg tctatagtgg ctaggtaccc tttttgtttt ggacacatgt
     7621 agggtggccg aaacaaagta ataggacaac aacgctcgac cgcgattatt tttggagaat
     7681 catgacctca gcatctgccc caagctttaa ccccggcaag ggtcccggct cagcagtcgg
     7741 aattgccctt ttaggattcg gaacagtcgg cactgaggtg atgcgtctga tgaccgagta
     7801 cggtgatgaa cttgcgcacc gcattggtgg cccactggag gttcgtggca ttgctgtttc
     7861 tgatatctca aagccacgtg aaggcgttgc acctgagctg ctcactgagg acgcttttgc
     7921 actcatcgag cgcgaggatg ttgacatcgt cgttgaggtt atcggcggca ttgagtaccc
     7981 acgtgaggta gttctcgcag ctctgaaggc cggcaagtct gttgttaccg ccaataaggc
     8041 tcttgttgca gctcactctg ctgagcttgc tgatgcagcg gaagccgcaa acgttgacct
     8101 gtacttcgag gctgctgttg caggcgcaat tccagtggtt ggcccactgc gtcgctccct
     8161 ggctggcgat cagatccagt ctgtgatggg catcgttaac ggcaccacca acttcatctt
     8221 ggacgccatg gattccaccg gcgctgacta tgcagattct ttggctgagg caactcgttt
     8281 gggttacgcc gaagctgatc caactgcaga cgtcgaaggc catgacgccg catccaaggc
     8341 tgcaattttg gcatccatcg ctttccacac ccgtgttacc gcggatgatg tgtactgcga
     8401 aggtatcagc aacatcagcg ctgccgacat tgaggcagca cagcaggcag gccacaccat
     8461 caagttgttg gccatctgtg agaagttcac caacaaggaa ggaaagtcgg ctatttctgc
     8521 tcgcgtgcac ccgactctat tacctgtgtc ccacccactg gcgtcggtaa acaagtcctt
     8581 taatgcaatc tttgttgaag cagaagcagc tggtcgcctg atgttctacg gaaacggtgc
     8641 aggtggcgcg ccaaccgcgt ctgctgtgct tggcgacgtc gttggtgccg cacgaaacaa
     8701 ggtgcacggt ggccgtgctc caggtgagtc cacctacgct aacctgccga tcgctgattt
     8761 cggtgagacc accactcgtt accacctcga catggatgtg gaagatcgcg tgggggtttt
     8821 ggctgaattg gctagcctgt tctctgagca aggaatctcc ctgcgtacaa tccgacagga
     8881 agagcgcgat gatgatgcac gtctgatcgt ggtcacccac tctgcgctgg aatctgatct
     8941 ttcccgcacc gttgaactgc tgaaggctaa gcctgttgtt aaggcaatca acagtgtgat
     9001 ccgcctcgaa agggactaac tgaactcctc aacgttatgg ctattgtgtt gcctaacttg
     9061 gtgcgacttc aattgtgctc ttaaattcca gttgttgcca cgcttcgccc gcctgccagg
     9121 gagtttcagg cccgggtggg tactcggtac caaacaggga tgcccacttt tgggatttcc
     9181 gtcgttgctg ggcagtctgg tttggtttca gcccgtcgaa accaaacaca gatgcccacc
     9241 gatcgcctat ttaaacatcg tgggcacgcc tgtctggtcg cctcgaaaac tggccaaatt
     9301 tagaccccga ccccacaaaa agtgacctca cagaatcgcc tctaagcgcc tcaaagaacc
     9361 cgacccacgc tgacagtccc ctacgatttc cgacgtctta aatcgccaca cagcgccgtg
     9421 gtctaaaaca ccaacaaaag agttgtaact gtaccgacca ttcgttacag ttacgatcca
     9481 tgacttcaga aaccttacag gcgcaagcgc ctacgaaaac ccaacgttgg gctttcctcg
     9541 ccgttatcag cggtggtctc tttctgatcg gtgtagacaa ctcgattctc tacaccgcac
     9601 tccctctgct gcgtgaacag ctcgcagcca ccgaaaccca agcgttgtgg atcatcaacg
     9661 catatcccct gctcatggcg ggccttcttt tgggtaccgg cactttgggt gacaaaatcg
     9721 gccaccgccg gatgttcctc atgggcttga gcattttcgg aatcgcttca cttggtgctg
     9781 cgtttgctcc aactgcgtgg gctcttgttg ctgcgagagc tttccttggc atcggtgcgg
     9841 caacgatgat gcctgcaacc ttggctctga tccgcattac gtttgaggat gagcgtgagc
     9901 gcaacactgc aattggtatt tggggttccg tggcaattct tggcgctgcg gcaggcccga
     9961 tcattggtgg tgcgctgttg gaattcttct ggtggggttc ggttttcctc attaacgttt
    10021 ctttcagcct ggcggtgtaa tgcaccgcca acttgaaagt taacaggtga acagttgttc
    10081 tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag
    10141 atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat
    10201 gagaacgaac cattgagatc atgcttactt tgcatgtcac tcaaaaattt tgcctcaaaa
    10261 ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt
    10321 aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg
    10381 ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta
    10441 tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct
    10501 ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca
    10561 agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc
    10621 ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa
    10681 gacttaacat gttccagatt atattttatg aattttttta actggaaaag ataaggcaat
    10741 atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac
    10801 tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc
    10861 tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga
    10921 gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg
    10981 gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag
    11041 cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg
    11101 tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata atta
//
