LOCUS       sequence-458198-       11128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing ISClsp3 controled by PlacM promoter, guide RNA controled 
            by PJ23119 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Wang X, Yang S, Qian F, Dong F, Zhou X, Yin M, Zhang Y, Huang Z, 
            Jiang Y, Yang S
  TITLE     RAGATH-Associated DNA Nuclease Assisted DNA Insertion in 
            Corynebacterium glutamicum.
  JOURNAL   ACS Synth Biol. 2025 May 8. doi: 10.1021/acssynbio.5c00022.
  PUBMED    40338137
REFERENCE   2  (bases 1 to 11128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2025 May 8. doi: 10.1021/acssynbio.5c00022."
COMMENT     Sequence Label: pISClsp3-reRNA
FEATURES             Location/Qualifiers
     source          1..11128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3671..4465
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3725..3744)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4335..4354
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      complement(5765..5792)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(5884..5968)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     ncRNA           complement(5973..6040)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     CDS             complement(10111..11061)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      join(11109..11128,1..203)
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt tgctggaaaa
       61 cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt
      121 tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata
      181 gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg
      241 tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc
      301 accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct
      361 gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg
      421 gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca
      481 agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat
      541 ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat
      601 tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa
      661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
      721 aaaggatctt cacctagatc ctttacgctc cgggacgcca acaacaagac ccatcatagt
      781 ttgcccccgc gacattgacc ataaattcat cgcacaaaat atcgaacggg gtttatgccg
      841 cttttagtgg gtgcgaagaa tggtctgctc attacccgcg aacaccgccg cattcagatc
      901 acgcttagta gcgtccccat gagtaggcag aaccgcgtcc aagtccacat catccataac
      961 gatcatgcac ggggtggaat ccacacccag acttgccagc acctcattag cgacacgttg
     1021 cgcagcggcc acgtccttag ccttatccac gcaatcgaga acgtactgcc taaccgcgaa
     1081 atcagactga atcagtttcc aatcatcggg cttcaccaaa gcaacagcaa cgcgggttga
     1141 ttcgacccgt tccggtgctt ccagaccggc gagcttgtac agttcttctt ccatttcacg
     1201 acgtacatca gcgtctatgt aatcaatgcc caaagcacgc ttagccccac gtgaccagga
     1261 cgaacgcagg tttttagaac caacctcata ctcacgccac cgagccacca aaacagcgtc
     1321 catatcctcg ccggcgtcgc tttgatcggc caacatatcc aacatctgaa acggcgtgta
     1381 cgacccctta gacgcggttt tagtagcgga gccagtcagt tcctgagaca tgcccttagc
     1441 gaggtaggtt gccattttcg cagcgtctcc accccaggta gacacctgat caagtttgac
     1501 cccgtgctca cgcagtggcg cgtccatacc ggccttaacc acaccagcag accagcggga
     1561 aaacatggaa tcctcaaacg ccttgagttc atcgtcagac agtggacgat ccaagaacaa
     1621 cagcatgttg cggtgcaagt gccaaccgtt cgcccaagag tctgtgacct catagtcact
     1681 ataggtgtgc tccaccccgt accgtgcacg ttctttcttc cactgagatg ttttcaccat
     1741 cgaagagtac gcagtcttaa tacccgcttc aacctgcgca aatgactgtg agcggttgtg
     1801 tcgaacagtg cccacaaaca tcatgagcgc gccacccgcc gccaagtgat tcttagtagc
     1861 aatagccagc tcaatgcggc gttcgcccat gacttccaat tcagccagag atgaccccca
     1921 gcgagagtga gagttttgca gaccctcaaa ctgcgaagca ccgttagacg accaggacac
     1981 cgcaacagct tcgtccctgc gccacctatg gcaccccgcc agagccttac tattggtgat
     2041 cttgtacatg acgttttgcc tacgccacgc cctagcgcga gtgaccttag aaccctcatt
     2101 gacctgcggt tccttagagg tgttcacttc tatttcagtg ttacctagac ccgatgttgt
     2161 gcggggttgc gcagtgcgag tttgtgcggg tgttgtgccc gttgtcttag ctagtgctat
     2221 ggttgtcaat tgaaacccct tcgggttatg tggcccccgt gcatatgagt tggtagctcg
     2281 cacgggggtt tgtcttgtct agggactatt aatttttagt ggtgtttggt ggccgcctag
     2341 cttggctatg cgtgccagct tacccgtact caatgttaaa gatttgcatc gacatgggag
     2401 ggttacgtgt ccgataccta gggggggtat ccgcgactag gtgccccggt gctcactgtc
     2461 tgtaccggcg gggcaagccc cacaccccgc atggacaggg tggctccgcc ccctgcaccc
     2521 ccagcaatct gcatgtacat gttttacaca ttagcacgac atgactgcat gtgcatgcac
     2581 tgcatgcaga ctaggtaaat atgagtatgt acgactagta acaggagcac tgcacataat
     2641 gaatgagttg caggacaatg tttgctacgc atgcgcatga catatcgcag gaaagctact
     2701 agagtcttaa agcatggcaa ccaaggcaca gctagaacag caactacaag aagctcaaca
     2761 ggcactacag gcgcagcaag cgcaggcaca agccaccatc gaagcactag aagcgcaggc
     2821 aaaggctaag cccgtcgtgg tcaccgcacg cgttcctttg gcactacgtg aggacatgaa
     2881 gcgcgcaggc atgcagaacg gtgaaaacct ccaagagttc atgatcgccg cgtttaccga
     2941 gcggctagaa aagctcacca ccaccgacaa cgaggaaaac aatgtctaac ccactagttc
     3001 tctttgccca ccgtgacccg gtaaatgacg tgacgttcga gtgcattgag cacgccacct
     3061 acgacacact ttcacacgct aaagaccaga tcaccgccca aatgcaagcc ctagacgaag
     3121 aagccgccct actgccctaa tgggtgtttc atgggtgttt ccctagtgtt tcatggtgtt
     3181 ttcacctaag ctagggaatt gcgcgagaag tctcgcaaaa atcagcaacc cccggaacca
     3241 cacagttcac gggggttctt ctatgccaga aatcagaaag gggaaccagt gaacgacccc
     3301 gaatggctgg atgatcctcc ttgggccacg cccacccggc gatcatcgag gcggtcaccg
     3361 gaagcggaac acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     3421 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     3481 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     3541 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     3601 cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg
     3661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     3721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     3781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     3841 gaatgaactc caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     3901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     3961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4201 gatgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcggaatc atgacctgag
     4501 ctgtttacaa ttaatcatcg tgtggtacca tgtgtggaat tggaaaggac ttgaacgatg
     4561 cgcaaactgc tgaaaagctt caaaaccgaa atcaacccga ccgaagaaca gaaaaccaaa
     4621 atccgcaaaa ccatcggcac ctgccgcttc atctacaact tctacctggc ccacaacaaa
     4681 aaactgcacg aagatggcaa aaaattcatg agcagcaaca aattccgcat ctggctgaac
     4741 aacgaatacc tgccgcagca cccggaatac agctggatca aagaagccta cagcaaagcc
     4801 gtgacacaga gcgtgaacaa cggtcagacc gccttcaccc gcttcttcaa ccacgaaagc
     4861 gccttcccga acttcaaaaa gaaaggcaaa agcgatgtga aaatgtactt cgtgaaaaac
     4921 aacccgaaag attgccactg cgaacgccac cgcatcaaaa tcccgagcct gggctgggtg
     4981 cgcatcaaag aaaaaggcta catcccgacc accaaagatg gctacgtgat caaaagcggc
     5041 agcgtgagca tcaaagccga tcgctactac gtgagcgtgc tggtggaagt gagcgataac
     5101 aaaaccatca accacttcga tgaaggcatc ggcatcgatc tgggcctgaa agatttcgcc
     5161 atcgtgagca acggccgcac ctatcagaat atcaacaaaa gcgccaaact gaaaaaactg
     5221 gaacgtcagc tgatccgcga acagcgctgc ctgagccgca aatacgaaaa cctgaaaaaa
     5281 ggcgatgcca cacagcgcgc caacattcag aaacagaaac tgaaagtgca gaaactgcac
     5341 caccgcatcg ataacatccg caccgattac atcaacaaaa caattgccga aatggtgaaa
     5401 atcaaaccga gctacatcac catcgaagat ctgaacgtga aaggcatgat gaaaaaccgc
     5461 cacctgtcca aagccgtggc gagccaaaaa ttctacgaat tccgcgccaa actgctggcc
     5521 aaatgcaacg aaagcggcat cgaactgcgc gtggtggatc gctggtaccc gagcagcaaa
     5581 acctgccact gctgcggcag catcaaaaaa gatctgaaac tgagcgatcg catcttcaaa
     5641 tgccgctgcg gctacatcga agatcgcgat ttcaacgccg ccctgaacct gcgcgatgcc
     5701 gaaacctaca gcatcgccta agtcgacctg caggcatgca agcttaagag tttgtagaaa
     5761 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
     5821 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgttcccgg
     5881 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     5941 gtctttcgac tgagctttcg ttttatttaa atgtcccatt cgccatgccg aagcatgttg
     6001 cccagccggc gccagcgagg aggctgggac catgccggcc ttccactatt tcttctggca
     6061 ctgccactca aaatgtgatt aaatataccc acatcgagat aatgtaagtt ctcgcgaaca
     6121 ttacttactg cttcattgtg tatgtttttg attgaccgta gtcatatcta ctcacaagtt
     6181 catgtacact ccacagtcgt aaattcccga aatagccttc ggtacatact tacatttgcg
     6241 ttttaaggat ccattatacc taggactgag ctagctgtca actaattgtt tctgccgcgg
     6301 gcaactacct tccaggtacg gaaagtgcca tcggcttccc gaacgtagac ctcatccacc
     6361 aaattaatca cgttgcaggc atggttgggc acgatgttga gctgctcccc gattactgga
     6421 agtagcactt tatctggcca gaaaatggtt gcgtgatgct ccgacaaagc agagattcgg
     6481 gcttcaggat tccccagaac aaaaccattg ccatcaatcc atgctggttt atcagtgctg
     6541 aggattttgg atcccgcatc caaaatgatc cgacgatctg acacatttcg gctgaccaca
     6601 gtagacagca ccgtcattgc cacctgcttc tcagtgcatg ctcccgaggt gatctgctgg
     6661 gaatcgttaa acacatatac gcctggtcgc atctcatcga ttgcgtctgt aaactgcgca
     6721 gacggcgagg aaccgccaga agtcaggccg ccagcaagtc gctggacgct gttgtttaga
     6781 gcctgaagct catcagctgc tgcctgctca ccatttcccg ggccataaga atgcccagga
     6841 aaagtaaaca ctcctgcata cctgctgccc agcgcctcgc ggatctgact caattctgaa
     6901 gcagtcgccg tgactccact tctacgatgt cccgaatcca cttcaatcag agccttgata
     6961 tcttcccgca aacccgccgt cgcctgtgcc atctctaccg aatccacgcc aatggaaatt
     7021 tctccgggga tcgcgttcag gcgttgcact gcatgatcgg ttagatacag cggatatgca
     7081 ataaagatgt ccgtaaaacc tgcgccggca aaaatttccg cctcgccaat ggttgcgcag
     7141 gtgatccctc gggcaccggc gtcgacctgc atctgcgcaa tttcaatgat tttgtgcgtt
     7201 ttcacatgcg gacgcagggc aatctcatgg gcaccggcgt gagctgccat cctggaaatg
     7261 ttggcagtta agcgctcgcg gtcaatgaga acagcaggtg tatcaatcat catggagtag
     7321 gaatattcct tatgttcgaa cgggccagat cgatcatcgc tcctactcct ccaccaaaga
     7381 cggttcgggt ggccgccttg ctgaatccca tgacctgttc ccacaatgtc ctccccgggt
     7441 tgatattaga tttcataaat atactaaaaa tcttgagagt ttttccgttg aaaactaaaa
     7501 agctgggaag gtgaatcgaa tttcggggct ttaaagcaaa aatgaacagc ttggtctata
     7561 gtggctaggt accctttttg ttttggacac atgtagggtg gccgaaacaa agtaatagga
     7621 caacaacgct cgaccgcgat tatttttgga gaatcatgac ctcagcatct gccccaagct
     7681 ttaaccccgg caagggtccc ggctcagcag tcggaattgc ccttttagga ttcggaacag
     7741 tcggcactga ggtgatgcgt ctgatgaccg agtacggtga tgaacttgcg caccgcattg
     7801 gtggcccact ggaggttcgt ggcattgctg tttctgatat ctcaaagcca cgtgaaggcg
     7861 ttgcacctga gctgctcact gaggacgctt ttgcactcat cgagcgcgag gatgttgaca
     7921 tcgtcgttga ggttatcggc ggcattgagt acccacgtga ggtagttctc gcagctctga
     7981 aggccggcaa gtctgttgtt accgccaata aggctcttgt tgcagctcac tctgctgagc
     8041 ttgctgatgc agcggaagcc gcaaacgttg acctgtactt cgaggctgct gttgcaggcg
     8101 caattccagt ggttggccca ctgcgtcgct ccctggctgg cgatcagatc cagtctgtga
     8161 tgggcatcgt taacggcacc accaacttca tcttggacgc catggattcc accggcgctg
     8221 actatgcaga ttctttggct gaggcaactc gtttgggtta cgccgaagct gatccaactg
     8281 cagacgtcga aggccatgac gccgcatcca aggctgcaat tttggcatcc atcgctttcc
     8341 acacccgtgt taccgcggat gatgtgtact gcgaaggtat cagcaacatc agcgctgccg
     8401 acattgaggc agcacagcag gcaggccaca ccatcaagtt gttggccatc tgtgagaagt
     8461 tcaccaacaa ggaaggaaag tcggctattt ctgctcgcgt gcacccgact ctattacctg
     8521 tgtcccaccc actggcgtcg gtaaacaagt cctttaatgc aatctttgtt gaagcagaag
     8581 cagctggtcg cctgatgttc tacggaaacg gtgcaggtgg cgcgccaacc gcgtctgctg
     8641 tgcttggcga cgtcgttggt gccgcacgaa acaaggtgca cggtggccgt gctccaggtg
     8701 agtccaccta cgctaacctg ccgatcgctg atttcggtga gaccaccact cgttaccacc
     8761 tcgacatgga tgtggaagat cgcgtggggg ttttggctga attggctagc ctgttctctg
     8821 agcaaggaat ctccctgcgt acaatccgac aggaagagcg cgatgatgat gcacgtctga
     8881 tcgtggtcac ccactctgcg ctggaatctg atctttcccg caccgttgaa ctgctgaagg
     8941 ctaagcctgt tgttaaggca atcaacagtg tgatccgcct cgaaagggac taactgaact
     9001 cctcaacgtt atggctattg tgttgcctaa cttggtgcga cttcaattgt gctcttaaat
     9061 tccagttgtt gccacgcttc gcccgcctgc cagggagttt caggcccggg tgggtactcg
     9121 gtaccaaaca gggatgccca cttttgggat ttccgtcgtt gctgggcagt ctggtttggt
     9181 ttcagcccgt cgaaaccaaa cacagatgcc caccgatcgc ctatttaaac atcgtgggca
     9241 cgcctgtctg gtcgcctcga aaactggcca aatttagacc ccgaccccac aaaaagtgac
     9301 ctcacagaat cgcctctaag cgcctcaaag aacccgaccc acgctgacag tcccctacga
     9361 tttccgacgt cttaaatcgc cacacagcgc cgtggtctaa aacaccaaca aaagagttgt
     9421 aactgtaccg accattcgtt acagttacga tccatgactt cagaaacctt acaggcgcaa
     9481 gcgcctacga aaacccaacg ttgggctttc ctcgccgtta tcagcggtgg tctctttctg
     9541 atcggtgtag acaactcgat tctctacacc gcactccctc tgctgcgtga acagctcgca
     9601 gccaccgaaa cccaagcgtt gtggatcatc aacgcatatc ccctgctcat ggcgggcctt
     9661 cttttgggta ccggcacttt gggtgacaaa atcggccacc gccggatgtt cctcatgggc
     9721 ttgagcattt tcggaatcgc ttcacttggt gctgcgtttg ctccaactgc gtgggctctt
     9781 gttgctgcga gagctttcct tggcatcggt gcggcaacga tgatgcctgc aaccttggct
     9841 ctgatccgca ttacgtttga ggatgagcgt gagcgcaaca ctgcaattgg tatttggggt
     9901 tccgtggcaa ttcttggcgc tgcggcaggc ccgatcattg gtggtgcgct gttggaattc
     9961 ttctggtggg gttcggtttt cctcattaac gtttctttca gcctggcggt gtaatgcacc
    10021 gccaacttga aagttaacag gtgaacagtt gttctacttt tgtttgttag tcttgatgct
    10081 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
    10141 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt
    10201 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
    10261 agcatcgtgt agtgtttttc ttagtccgtt atgtaggtaa gaatctgatg taatggttgt
    10321 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
    10381 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
    10441 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
    10501 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
    10561 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
    10621 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
    10681 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
    10741 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
    10801 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
    10861 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
    10921 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
    10981 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
    11041 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
    11101 attgagatgg gctagtcaat gataatta
//
