LOCUS       sequence-458199-        9139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A gateway compatible destination vector containing the Xenopous 
            myosin light chain 2 promoter for embryonic and adult cardiomyocyte 
            expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Gillespie WB 3rd, Zhang Y, Ruiz OE, Cerda J 3rd, Ortiz-Guzman J, 
            Sherman M, Turner WD, Largoza G, Mosser LE, Fujimoto E, Chien CB, 
            Kwan KM, Arenkiel BR, Devine WP, Wythe JD
  TITLE     MultiSite Assembly of Gateway Induced Clones (MAGIC): a flexible 
            cloning toolbox for use in vertebrate model systems.
  JOURNAL   Development. 2025 Nov 15;152(22):dev204308. doi: 10.1242/dev.204308.
            Epub 2025 Nov 13.
  PUBMED    41230856
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2025-11-15"; volume: "152(22):dev204308. 
            doi"; pages: " 10.1242/dev.204308. Epub 2025 Nov 13"
COMMENT     Sequence Label: pXMLC2-DEST (JDW 7)
FEATURES             Location/Qualifiers
     source          1..9139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(166..185)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    3407..3531
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(3556..3561,3562..3579,3580..3586)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3640..4299
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3706..3725)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             4641..4946
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4825..4844
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(4987..5111)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5137..5178
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5188..5205
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5228..5245)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5234..5458
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5504..5932
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5591..5610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5801..5822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5941..5961)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5946..6275
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6126..6261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6188..6207
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6323..6370
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6389..6787
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6945..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6982..7001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7036..7055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7115..7131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7115..7131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7128..7150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7163..7169,7170..7187,7188..7193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7346..7363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7506..8094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7586..7605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8265..9056,9057..9125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8888..8907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9126..9139,1..91))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      361 ttgattattg actaggcttt tgcaaaaagc ttctagaatc atattcagtg tttctagttt
      421 atataaacat ttgaggagta attattgttg gatttcacgc tgactttggc tcaggtcaaa
      481 aaattgccta ctattcctca gacacagcct acagatggaa gagacacggg agcaatattt
      541 acaataaaag acaatgtaaa ctattctact tgcagacaga aaaaacagtt ctgaacttga
      601 agctggacat tataatgagt agccagaaag tgatgaagta actggaaaat gtttgcagtc
      661 aggggttata ggaacaagtg atttaatgct aaagtctgca gtatcaatac aaaaaaagga
      721 tttacattaa tattccacaa ttttaccagt aatatgtgat atcacaatga caatttgctg
      781 ttggtggtgt ctccgattct gctatgtttt ctatttttcg taaaaaaaga gctgctgaag
      841 gttgtcttaa atgcaacact gtgcaaaaaa aaacattatc taggtttatt gaaatcacag
      901 ccacagaaaa gcctaaggag ttcctaggaa ctaagagaga atattaggaa ggaaatatat
      961 ggcagcttgc aaaggattta tttcttctgg atccgtattt gtttgaccga tgtcacaatg
     1021 gtgatcgagt gttattacat gcaatgcatc tctttccatt agcatggaca gcggtttggc
     1081 tatttcactt tcatttaagc ctcaaaaaca ttgagaaatc ataacacaac atccacatga
     1141 ttataaaata gacagagttt tctgatggat ggaaactgta ttaaagactg aatacaatcc
     1201 aggctcattt cagtgggtcc ctatgtaaac tcttcttata ttgttaaagg tatacatgca
     1261 gaccatggat ctcttaatac tgtatctacc agcattcctg tgtgggaaat atactcatcc
     1321 acttttaaag ttactaaggg gccaagtgtc ccagaactaa atagaacttg gtcccttcca
     1381 gaatttcatg tcatgctggt ttaatggcat cattttttgt ctagttttgt aagatgcttt
     1441 tgtgtatgaa attagcagaa ttatacttat ctgttttgtt ttattgctat gttttcctga
     1501 ttgaatccta caagttacag agtatttgtt caatagattt actgatatca gtttgtttgt
     1561 tggaaacaat aaatataaca tcttgccagg tttgctttat gtcacattgt gatgtccaat
     1621 ggaaagcaca tggtggggct tatgtattaa aagatcacta atcactaaca tcagaaggca
     1681 gcatttaatg tttaaattca gacatcttat aggttgctat gggttactgc acctgggcaa
     1741 actctatagg ggtacattta tcaaagagtg aagttccccc actagagtga aattccacaa
     1801 ctcacaattc atttctatgg gattttgaaa ggcatattta tcaatgggtg aaagtgaaag
     1861 ttcacccttt gataaatagg cctttaaaaa tcccatagaa atgaatggaa atttcactct
     1921 agtggcggaa cttcactatt aacttcactc tttgataaat atacccctat gccttttatt
     1981 gcatatgggg actaattatt aaaattatta acatgtattt ttagagcgcc aacatattgt
     2041 gcagcgctct ttgtatacta ttatactgtt tggtcaggtt ttgattgaac tctaaatgag
     2101 taggttctga aaatgaattt gatcgccctc ttgtatggtc actccaaaga tggtaggcaa
     2161 aaacaaaagg accaatcatt agtacttctg ctggagcatt aactgctctg tagctgtgca
     2221 ggcttgcact gctgtctagt ggtatgccgg accctgttag aaaacatcaa tactaatggt
     2281 aaaaaaacat cagtgttcat atatactgta tatatatttt aggcaatatg ccattagcct
     2341 ttggatccac aggggaaatt ctgggtctgg tgctgcctga agctatctga ttaaataagg
     2401 taataaaaaa acaaatcagg tgatgttctg cctgtgacag caattgcatg aaagttatta
     2461 atcacaaata gcattgactg tcacccattg atattgtcag atacatacag agatattatc
     2521 gttagcaaac agaaatcttc taacctgtcc gatccactaa acgaccaatt gccatggtac
     2581 gaaaaatgtt ggggcgatcc acacatggtc caaaaatcgt aagaaacttt gatctgtacg
     2641 actatatctt tgcgtcgatg gccagcttta gaaaggagag cagggatatc agttttacct
     2701 ctcctcacct actgttatca actgaaaatg tcgaatgtgg ccaaggatat tagaagtagg
     2761 gttgccagcc agggtggcct ggccagtaaa aattatactt gatgccaatg ttattaatag
     2821 gaaaaaatgc caagaatata ggaaggccgg tatttttttt ccagaaaagg tggcaacctt
     2881 aattggaagt cgtagttcaa atataattta aacagagaag ctgagaaatg gtctccttca
     2941 gagattctga aacagtggtc ttctgcatac agtattatta tacattttgt aggatgctga
     3001 gaatcaaaat gttcagtatg ctggaagata ccaattcagc aatagacagg ggtgcacagc
     3061 tttcagccga caacatagac tattctcttc ctctgaccct gttctaaaat taaggtttgc
     3121 ttttgctaat attagaagtg gaggcaatga gcttttccag ccagggggta ctttcctgat
     3181 aaatgaagta tccagtgtct atagaaggat ggtgggatat tttactgaac acaatgagaa
     3241 tggaatgtta gcccttgtgc tcttatctct tccgtctctc tctagccatc tttggttctc
     3301 tgcctgaggc ctggagctat tttagctatg cctgagataa gaaggagtat aaaatgatca
     3361 agagaacttt ccattaggtg gcacaggacg aaatcaagct tgatcaacaa gtttgtacaa
     3421 aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt agattttgca
     3481 taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat ggcggccgca
     3541 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
     3601 taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
     3661 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
     3721 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
     3781 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
     3841 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
     3901 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
     3961 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
     4021 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
     4081 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
     4141 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
     4201 gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa
     4261 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc cggcttacta
     4321 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
     4381 gatatgtata cccgaagtat gtcaaaaaga ggtatgctat gaagcagcgt attacagtga
     4441 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
     4501 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
     4561 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
     4621 cgagaacagg ggctggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
     4681 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
     4741 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4801 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4861 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4921 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
     4981 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
     5041 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
     5101 tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     5161 ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt ttaaacccgc
     5221 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     5281 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     5341 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5401 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5461 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc
     5521 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5581 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5641 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5701 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5761 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5821 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5881 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5941 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6001 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6121 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6181 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6241 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6301 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     6361 ataatacgac aaggtgagga actaaaccat ggccaagcct ttgtctcaag aagaatccac
     6421 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     6481 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     6541 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     6601 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     6661 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     6721 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     6781 gggctaagca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     6841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9121 ctcatactct tcctttttc
//
