LOCUS       sequence-458206-        9103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Mi LZ, Grey MJ, Nishida N, Walz T, Lu C, Springer TA
  TITLE     Functional and structural stability of the epidermal growth factor 
            receptor in detergent micelles and phospholipid nanodiscs.
  JOURNAL   Biochemistry. 2008 Sep 30;47(39):10314-23. doi: 10.1021/bi801006s. 
            Epub 2008 Sep 5.
  PUBMED    18771282
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi801006s";
            journalName: "Biochemistry"; date: "2008-09-30- 30"; volume: "47"; 
            issue: "39"; pages: "10314-23"
COMMENT     Sequence Label: EGFR-ET1
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4267..4302
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4312..4329
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(4462..5036,5037..5039,5040..5048)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4629..4646)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4856..4875
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5049..5051,5052..5054,5055..5768)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5049..5051,5052..5054,5055..5765)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5094..5115)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5355..5374)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5702..5723
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5891..6012
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5928..5947)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5982..6001
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6019..6474)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6156..6177)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6368..6387
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6501..6605
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6607..6964
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6607..6798
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6630..6650)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6815..6950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6999..7793
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6999..7793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7053..7072)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7663..7682
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7981..8000)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8025..8072
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8401..8989
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8890..8909
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgtcg
      601 acgtacgcca ccatggatat gagagttcct gctcaattgc tggggttgct cttgctctgg
      661 tttcctggct cgagatgcct ggaggaaaag aaagtttgcc aaggcacgag taacaagctc
      721 acgcagttgg gcacttttga agatcatttt ctcagcctcc agaggatgtt caataactgt
      781 gaggtggtcc ttgggaattt ggaaattacc tatgtgcaga ggaattatga tctttccttc
      841 ttaaagacca tccaggaggt ggctggttat gtcctcattg ccctcaacac agtggagcga
      901 attcctttgg aaaacctgca ggtcatcaga ggaaatatgt actacgaaaa ttcctatgcc
      961 ttagcagtct tatctaacta tgatgcaaat aaaaccggac tgaaggagct gcccatgaga
     1021 aatttacagg aaatcctgca tggcgccgtg cggttcagca acaaccctgc cctgtgcaac
     1081 gtggagagca tccagtggcg ggacatagtc agcagtgact ttctcagcaa catgtcgatg
     1141 gacttccaga accacctagg cagctgccaa aagtgtgatc caagctgtcc caatgggagc
     1201 tgctggggtg caggagagga gaactgccag aaactgacca aaatcatctg tgcccagcag
     1261 tgctccgggc gctgccgtgg caagtccccc agtgactgct gccacaacca gtgtgctgca
     1321 ggctgcacag gcccccggga gagcgactgc ctggtctgcc gcaaattccg aaacgaagcc
     1381 acgtgcaagg acacctgccc cccactcatg ctctacaacc ccaccacgta ccagatggat
     1441 gtgaaccccg agggcaaata cagctttggt gccacctgcg tgaagaagtg tccccgtaat
     1501 tatgtggtga cagatcacgg ctcgtgcgtc cgagcctgtg gggccgacag ctatgagatg
     1561 gaggaagacg gcgtccgcaa gtgtaagaag tgcgaagggc cttgccgcaa agtgtgtaac
     1621 ggaataggta ttggtgaatt taaagactca ctctccataa atgctacgaa tattaaacac
     1681 ttcaaaaact gcacctccat cagtggcgat ctccacatcc tgccggtggc atttaggggt
     1741 gactccttca cacatactcc tcctctggat ccacaggaac tggatattct gaaaaccgta
     1801 aaggaaatca cagggttttt gctgattcag gcttggcctg aaaacaggac ggacctccat
     1861 gcctttgaga acctagaaat catacgcggc aggaccaagc aacatggtca gttttctctt
     1921 gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga gataagtgat
     1981 ggagatgtga taatttcagg aaacaaaaat ttgtgctatg caaatacaat aaactggaaa
     2041 aaactgtttg ggacctccgg tcagaaaacc aaaattataa gcaacagagg tgaaaacagc
     2101 tgcaaggcca caggccaggt ctgccatgcc ttgtgctccc ccgagggctg ctggggcccg
     2161 gagcccaggg actgcgtctc ttgccggaat gtcagccgag gcagggaatg cgtggacaag
     2221 tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg catacagtgc
     2281 cacccagagt gcctgcctca ggccatgaac atcacctgca caggacgggg accagacaac
     2341 tgtatccagt gtgcccacta cattgacggc ccccactgcg tcaagacctg cccggcagga
     2401 gtcatgggag aaaacaacac cctggtctgg aagtacgcag acgccggcca tgtgtgccac
     2461 ctgtgccatc caaactgcac ctacggatgc actgggccag gtcttgaagg ctgtccaacg
     2521 aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct cttgctgctg
     2581 gtggtggccc tggggatcgg cctcttcatg cggaggcgcc acatcgttcg gaagcgcacg
     2641 ctgcggaggc tgctgcagga gagggagctt gtggagcctc ttacacccag tggagaagct
     2701 cccaaccaag ctctcttgag gatcttgaag gaaactgaat tcaaaaagat caaagtgctg
     2761 ggctccggtg cgttcggcac ggtgtataag ggactctgga tcccagaagg tgagaaagtt
     2821 aaaattcccg tcgctatcaa ggaattaaga gaagcaacat ctccgaaagc caacaaggaa
     2881 atcctcgatg aagcctacgt gatggccagc gtggacaacc cccacgtgtg ccgcctgctg
     2941 ggcatctgcc tcacctccac cgtgcaactc atcacgcagc tcatgccctt cggctgcctc
     3001 ctggactatg tccgggaaca caaagacaat attggctccc agtacctgct caactggtgt
     3061 gtgcagatcg caaagggcat gaactacttg gaggaccgtc gcttggtgca ccgcgacctg
     3121 gcagccagga acgtactggt gaaaacaccg cagcatgtca agatcacaga ttttgggctg
     3181 gccaaactgc tgggtgcgga agagaaagaa taccatgcag aaggaggcaa agtgcctatc
     3241 aagtggatgg cattggaatc aattttacac agaatctata cccaccagag tgatgtctgg
     3301 agctacgggg tgaccgtttg ggagttgatg acctttggat ccaagccata tgacggaatc
     3361 cctgccagcg agatctcctc catcctggag aaaggagaac gcctccctca gccacccata
     3421 tgtaccatcg atgtctacat gatcatggtc aagtgctgga tgatagacgc agatagtcgc
     3481 ccaaagttcc gtgagttgat catcgaattc tccaaaatgg cccgagaccc ccagcgctac
     3541 cttgtcattc agggggatga aagaatgcat ttgccaagtc ctacagactc caacttctac
     3601 cgtgccctga tggatgaaga agacatggac gacgtggtgg atgccgacga gtacctcatc
     3661 ccacagcagg gtttcttcag cagcccctcc acgtcacgga ctcccctcct gagctctctg
     3721 agtgcaacca gcaacaattc caccgtggct tgcattgata gaaatgggct gcaaagctgt
     3781 cccatcaagg aagacagctt cttgcagcga tacagctcag accccacagg cgccttgact
     3841 gaggacagca tagacgacac cttcctccca gtgcctgaat acataaacca gtccgttccc
     3901 aaaaggcccg ctggctctgt gcagaatcct gtctatcaca atcagcctct gaaccccgcg
     3961 cccagcagag acccacacta ccaggacccc cacagcactg cagtgggcaa ccccgagtat
     4021 ctcaacactg tccagcccac ctgtgtcaac agcacattcg acagccctgc ccactgggcc
     4081 cagaaaggca gccaccaaat tagcctggac aaccctgact accagcagga cttctttccc
     4141 aaggaagcca agccaaatgg catctttaag ggctccacag ctgaaaatgc agaataccta
     4201 agggtcgcgc cacaaagcag tgaatttatt ggagcaacgc gtgaactgta cttccaaggt
     4261 ccagttgaag atcaagttga tccaagattg attgatggca agggctccgg tcaccatcac
     4321 catcaccacg gcaccactgg ttggagaggt ggccatgttg tggaaggctt ggctggtgaa
     4381 ttggaacaat tgagagcccg tctggagcat cacccacaag gccaaagaga accagcgtaa
     4441 actagtggcg cgccggatcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     4501 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     4561 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     4621 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     4681 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     4741 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     4801 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     4861 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     4921 atctgatctg gggcctcggt acacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     4981 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataatatgg
     5041 ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     5101 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     5161 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     5221 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     5281 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     5341 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     5401 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     5461 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     5521 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     5581 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     5641 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     5701 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     5761 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     5821 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     5881 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5941 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     6001 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     6061 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     6121 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     6181 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     6241 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     6301 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     6361 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     6421 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     6481 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     6541 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     6601 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     6661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     6721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6781 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     6961 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     7021 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     7081 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     7141 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     7201 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     7261 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     7321 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     7381 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     7441 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     7501 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     7561 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     7621 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     7681 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     7741 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     7801 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7861 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7921 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     7981 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     8041 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     8101 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     8161 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     8221 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     8281 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8341 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8401 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8461 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8521 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8581 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8641 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8701 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8761 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     8821 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     8881 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8941 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9001 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9061 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
