LOCUS       sequence-458289-       11998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of iRFP670-alpha-cateninA+ in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Noordstra I, Hermoso MD, Schimmel L, Bonfim-Melo A, Currin-Ross D, 
            Duong CN, Kalappurakkal JM, Morris RG, Vestweber D, Mayor S, Gordon 
            E, Roca-Cusachs P, Yap AS
  TITLE     An E-cadherin-actin clutch translates the mechanical force of 
            cortical flow for cell-cell contact to inhibit epithelial cell 
            locomotion.
  JOURNAL   Dev Cell. 2023 Sep 25;58(18):1748-1763.e6. doi: 
            10.1016/j.devcel.2023.06.011. Epub 2023 Jul 21.
  PUBMED    37480844
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.06.011"; journalName: "Dev Cell"; date: 
            "2023-09-25- 25"; volume: "58"; issue: "18"; pages: "1748-1763.e6"
COMMENT     Sequence Label: pLViN-iRFP670-alpha-cateninA+
FEATURES             Location/Qualifiers
     source          1..11998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2835..2844
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2841..3770
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQ"
     misc_feature    6597..7146
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6741..6758)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6968..6987
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7149..7943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7203..7222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7813..7832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7967..8555
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8020..8040)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8438..8449)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8762..9395
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9523..9539)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9523..9539)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9536..9558)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9547..9563
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9571..9577,9578..9595,9596..9601))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9616..9637
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9754..9771)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9925..10513)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10005..10024)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10684..11475,11476..11544))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11307..11326
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11545..11649)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11697..11831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11734..11753)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11788..11807
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tagctagagg atcccccggg ctgcaggaat tcagtgaacc
     2821 gtcagatccg ctaggccacc atggcgcgta aggtcgatct cacctcctgc gatcgcgagc
     2881 cgatccacat ccccggcagc attcagccgt gcggctgcct gctagcctgc gacgcgcagg
     2941 cggtgcggat cacgcgcatt acggaaaatg ccggcgcgtt ctttggacgc gaaactccgc
     3001 gggtcggtga gctactcgcc gattacttcg gcgagaccga agcccatgcg ctgcgcaacg
     3061 cactggcgca gtcctccgat ccaaagcgac cggcgctgat cttcggttgg cgcgacggcc
     3121 tgaccggccg caccttcgac atctcactgc atcgccatga cggtacatcg atcatcgagt
     3181 tcgagcctgc ggcggccgaa caggccgaca atccgctgcg gctgacgcgg cagatcatcg
     3241 cgcgcaccaa agaactgaag tcgctcgaag agatggccgc acgggtgccg cgctatctgc
     3301 aggcgatgct cggctatcac cgcgtgatgt tgtaccgctt cgcggacgac ggctccggga
     3361 tggtgatcgg cgaggcgaag cgcagcgacc tcgagagctt tctcggtcag cactttccgg
     3421 cgtcgctggt cccgcagcag gcgcggctac tgtacttgaa gaacgcgatc cgcgtggtct
     3481 cggattcgcg cggcatcagc agccggatcg tgcccgagca cgacgcctcc ggcgccgcgc
     3541 tcgatctgtc gttcgcgcac ctgcgcagca tctcgccctg ccatctcgaa tttctgcgga
     3601 acatgggcgt cagcgcctcg atgtcgctgt cgatcatcat tgacggcacg ctatggggat
     3661 tgatcatctg tcatcattac gagccgcgtg ccgtgccgat ggcgcagcgc gtcgcggccg
     3721 aaatgttcgc cgacttctta tcgctgcact tcaccgccgc ccaccaccaa cccggactca
     3781 gatctcgagc tcatatgact gctgtccatg caggcaacat aaacttcaag tgggatccta
     3841 aaagtctaga gatcaggact ctggcagttg agagactgtt ggagcctctt gttacacagg
     3901 ttacaaccct tgtaaacacc aatagtaaag ggccctctaa taagaagaga ggtcgttcta
     3961 agaaggccca tgttttggct gcatctgttg aacaagcaac tgagaatttc ttggagaagg
     4021 gggataaaat tgcgaaggag agccagtttc tcaaggagga gcttgtggct gctgtagaag
     4081 atgttcgaaa acaaggtgat ttgatgaagg ctgctgcagg agagttcgca gatgatccct
     4141 gctcttctgt gaagcgaggc aacatggttc gggcagctcg agctttgctc tctgctgtta
     4201 cccggttgct gattttggct gacatggcag atgtctacaa attacttgtt cagctgaaag
     4261 ttgtggaaga tggtatcttg aagttgagga atgctggcaa tgaacaagac ttaggaatcc
     4321 agtataaagc cctaaaacct gaagtggata agctgaacat tatggcagcc aaaagacaac
     4381 aggaattgaa agatgttggc catcgtgatc agatggctgc agctagagga atcctgcaga
     4441 agaacgttcc gatcctctat actgcatccc aggcatgcct acagcaccct gatgtcgcag
     4501 cctataaggc caacagggac ctgatataca agcagctgca gcaggcggtc acaggcattt
     4561 ccaatgcagc ccaggccact gcctcagacg atgcctcaca gcaccagggt ggaggaggag
     4621 gagaactggc atatgcactc aataactttg acaaacaaat cattgtggac cccttgagct
     4681 tcagcgagga gcgctttagg ccttccctgg aggagcgtct ggaaagcatc attagtgggg
     4741 ctgccttgat ggccgactcg tcctgcacgc gtgatgaccg tcgtgagcga attgtggcag
     4801 agtgtaatgc tgtccgccag gccctgcagg acctgctttc ggagtacatg ggcaatgctg
     4861 gacgtaaaga aagaagtgat gcactcaatt ctgcaataga taaaatgacc aagaagacca
     4921 gggacttgcg tagacagctc cgcaaagctg tcatggacca cgtttcagat tctttcctgg
     4981 aaaccaatgt tccacttttg gtattgattg aagctgcaaa gaatggaaat gagaaagaag
     5041 ttaaggagta tgcccaagtt ttccgtgaac atgccaacaa attgattgag gttgccaact
     5101 tggcctgttc catctcaaat aatgaagaag gtgtaaagct tgttcgaatg tctgcaagcc
     5161 agttagaagc cctctgtcct caggttatta atgctgcact ggctttagca gcaaaaccac
     5221 agagtaaact ggcccaagag aacatggatc tttttaaaga acaatgggaa aaacaagtcc
     5281 gtgttctcac agatgctgtc gatgacatta cttccattga tgacttcttg gctgtctcag
     5341 agaatcacat tttggaagat gtgaacaaat gtgtcattgc tctccaagag aaggatgtgg
     5401 atggcctgga ccgcacagct ggtgcaattc gaggccgggc agcccgggtc attcacgtag
     5461 tcacctcaga gatggacaac tatgagccag gagtctacac agagaaggtt ctggaagcca
     5521 ctaagctgct ctccaacaca gtcatgccac gttttactga gcaagtagaa gcagccgtgg
     5581 aagccctcag ctcggaccct gcccagccca tggatgagaa tgagtttatc gatgcttccc
     5641 gcctggtata tgatggcatc cgggacatca ggaaagcagt gctgatgata aggacccctg
     5701 aggagttgga tgactctgac tttgagacag aagattttga tgtcagaagc aggacgagcg
     5761 tccagacaga agacgatcag ctgatagctg gccagagtgc cgggagtggc agtgctcagc
     5821 ttccccagga gcaaaaagcg aagattgcgg aacaggtggc cagcttccag gaagaaaaga
     5881 gcaagctgga tgctgaagtg tccaaatggg acgacagtgg caatgacatc attgtgctgg
     5941 ccaagcagat gtgcatgatt atgatggaga tgacagactt tacccgaggt aaaggaccac
     6001 tcaaaaatac atcggatgtc atcagtgctg ccaagaaaat tgctgaggca ggatccagga
     6061 tggacaagct tggccgcacc attgcagacc attgccccga ctcggcttgc aagcaggacc
     6121 tgctggccta cctgcaacgc atcgccctct actgccacca gctgaacatc tgcagcaagg
     6181 tcaaggccga ggtgcagaat ctcggcgggg agcttgttgt ctctggggtg gacagcgcca
     6241 tgtccctgat ccaggcagcc aagaacttga tgaatgctgt ggtgcagaca gtgaaggcat
     6301 cctacgtcgc ctctaccaaa taccaaaagt cacagggtat ggcttccctc aaccttcctg
     6361 ctgtgtcatg gaagatgaag gcaccagaga aaaagccatt ggtgaagaga gagaaacagg
     6421 atgagacaca gaccaagatt aaacgggcat ctcagaagaa gcacgtgaac ccggtgcagg
     6481 ccctcagcga gttcaaagct atggacagca tctaatgcag tcgacggtac cgcgttaact
     6541 aacttaagct agcaacggtt tccctctagc gggatcaatt ccgccccccc cccctaacgt
     6601 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     6661 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     6721 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     6781 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     6841 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     6901 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     6961 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     7021 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     7081 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     7141 ataataccat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     7201 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     7261 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     7321 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     7381 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     7441 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     7501 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     7561 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     7621 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     7681 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     7741 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     7801 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     7861 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     7921 gccttcttga cgagttcttc tgacccgggc ggggcgcgtc tggaacaatc aacctctgga
     7981 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     8041 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     8101 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     8161 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8221 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8281 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8341 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     8401 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8461 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8521 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     8581 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     8641 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     8701 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     8761 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     8821 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
     8881 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
     8941 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     9001 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
     9061 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
     9121 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
     9181 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
     9241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9361 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9421 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt gacattgcta
     9481 gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     9541 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     9601 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     9661 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     9721 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     9781 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     9841 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     9901 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     9961 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
    10021 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
    10081 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
    10141 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
    10201 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
    10261 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
    10321 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
    10381 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
    10441 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
    10501 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
    10561 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
    10621 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
    10681 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
    10741 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
    10801 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
    10861 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
    10921 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
    10981 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
    11041 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
    11101 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
    11161 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
    11221 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
    11281 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
    11341 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
    11401 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
    11461 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
    11521 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
    11581 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
    11641 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
    11701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11821 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
    11881 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
    11941 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagt
//
