LOCUS       sequence-458291-       11722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of E-cadherin-GFPTG in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11722)
  AUTHORS   Noordstra I, Hermoso MD, Schimmel L, Bonfim-Melo A, Currin-Ross D, 
            Duong CN, Kalappurakkal JM, Morris RG, Vestweber D, Mayor S, Gordon 
            E, Roca-Cusachs P, Yap AS
  TITLE     An E-cadherin-actin clutch translates the mechanical force of 
            cortical flow for cell-cell contact to inhibit epithelial cell 
            locomotion.
  JOURNAL   Dev Cell. 2023 Sep 25;58(18):1748-1763.e6. doi: 
            10.1016/j.devcel.2023.06.011. Epub 2023 Jul 21.
  PUBMED    37480844
REFERENCE   2  (bases 1 to 11722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.06.011"; journalName: "Dev Cell"; date: 
            "2023-09-25- 25"; volume: "58"; issue: "18"; pages: "1748-1763.e6"
COMMENT     Sequence Label: pLViN-E-cadherin-GFP
FEATURES             Location/Qualifiers
     source          1..11722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5503..5512
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5509..5511,5512..5514,5515..6228)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5509..5511,5512..5514,5515..6225)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5554..5575)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5815..5834)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6162..6183
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6321..6870
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6465..6482)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6692..6711
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6873..7667
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6927..6946)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7537..7556
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7691..8279
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7744..7764)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8162..8173)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8486..9119
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9247..9263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9247..9263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9260..9282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9271..9287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9295..9301,9302..9319,9320..9325))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9340..9361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9478..9495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9649..10237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9729..9748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10408..11199,11200..11268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11031..11050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11269..11373)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11421..11555
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11458..11477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11512..11531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tagctagagg atcccccggg ctgcaggaat tcggcgcgcc
     2821 acgcgtccag ccatgggccc ttggagccgc agcctctcgg cgctgctgct gctgctgcag
     2881 gtctcctctt ggctctgcca ggagccggag ccctgccacc ctggctttga cgccgagagc
     2941 tacacgttca cggtgccccg gcgccacctg gagagaggcc gcgtcctggg cagagtgaat
     3001 tttgaagatt gcaccggtcg acaaaggaca gcctattttt ccctcgacac ccgattcaaa
     3061 gtgggcacag atggtgtgat tacagtcaaa aggcctctac ggtttcataa cccacagatc
     3121 catttcttgg tctacgcctg ggactccacc tacagaaagt tttccaccaa agtcacgctg
     3181 aatacagtgg ggcaccacca ccgccccccg ccccatcagg cctccgtttc tggaatccaa
     3241 gcagaattgc tcacatttcc caactcctct cctggcctca gaagacagaa gagagactgg
     3301 gttattcctc ccatcagctg cccagaaaat gaaaaaggcc catttcctaa aaacctggtt
     3361 cagatcaaat ccaacaaaga caaagaaggc aaggttttct acagcatcac tggccaagga
     3421 gctgacacac cccctgttgg tgtctttatt attgaaagag aaacaggatg gctgaaggtg
     3481 acagagcctc tggatagaga acgcattgcc acatacactc tcttctctca cgctgtgtca
     3541 tccaacggga atgcagttga ggatccaatg gagattttga tcacggtaac cgatcagaat
     3601 gacaacaagc ccgaattcac ccaggaggtc tttaaggggt ctgtcatgga aggtgctctt
     3661 ccaggaacct ctgtgatgga ggtcacagcc acagacgcgg acgatgatgt gaacacctac
     3721 aatgccgcca tcgcttacac catcctcagc caagatcctg agctccctga caaaaatatg
     3781 ttcaccatta acaggaacac aggagtcatc agtgtggtca ccactgggct ggaccgagag
     3841 agtttcccta cgtataccct ggtggttcaa gctgctgacc ttcaaggtga ggggttaagc
     3901 acaacagcaa cagctgtgat cacagtcact gacaccaacg ataatcctcc gatcttcaat
     3961 cccaccacgt acaagggtca ggtgcctgag aacgaggcta acgtcgtaat caccacactg
     4021 aaagtgactg atgctgatgc ccccaatacc ccagcgtggg aggctgtata caccatattg
     4081 aatgatgatg gtggacaatt tgtcgtcacc acaaatccag tgaacaacga tggcattttg
     4141 aaaacagcaa agggcttgga ttttgaggcc aagcagcagt acattctaca cgtagcagtg
     4201 acgaatgtgg taccttttga ggtctctctc accacctcca cagccaccgt caccgtggat
     4261 gtgctggatg tgaatgaagc ccccatcttt gtgcctcctg aaaagagagt ggaagtgtcc
     4321 gaggactttg gcgtgggcca ggaaatcaca tcctacactg cccaggagcc agacacattt
     4381 atggaacaga aaataacata tcggatttgg agagacactg ccaactggct ggagattaat
     4441 ccggacactg gtgccatttc cactcgggct gagctggaca gggaggattt tgagcacgtg
     4501 aagaacagca cgtacacagc cctaatcata gctacagaca atggttctcc agttgctact
     4561 ggaacaggga cacttctgct gatcctgtct gatgtgaatg acaacgcccc cataccagaa
     4621 cctcgaacta tattcttctg tgagaggaat ccaaagcctc aggtcataaa catcattgat
     4681 gcagaccttc ctcccaatac atctcccttc acagcagaac taacacacgg ggcgagtgcc
     4741 aactggacca ttcagtacaa cgacccaacc caagaatcta tcattttgaa gccaaagatg
     4801 gccttagagg tgggtgacta caaaatcaat ctcaagctca tggataacca gaataaagac
     4861 caagtgacca ccttagaggt cagcgtgtgt gactgtgaag gggccgccgg cgtctgtagg
     4921 aaggcacagc ctgtcgaagc aggattgcaa attcctgcca ttctggggat tcttggagga
     4981 attcttgctt tgctaattct gattctgctg ctcttgctgt ttcttcggag gagagcggtg
     5041 gtcaaagagc ccttactgcc cccagaggat gacacccggg acaacgttta ttactatgat
     5101 gaagaaggag gcggagaaga ggaccaggac tttgacttga gccagctgca caggggcctg
     5161 gacgctcggc ctgaagtgac tcgtaacgac gttgcaccaa ccctcatgag tgtcccccgg
     5221 tatcttcccc gccctgccaa tcccgatgaa attggaaatt ttattgatga aaatctgaaa
     5281 gcggctgata ctgaccccac agccccgcct tatgattctc tgctcgtgtt tgactatgaa
     5341 ggaagcggtt ccgaagctgc tagtctgagc tccctgaact cctcagagtc agacaaagac
     5401 caggactatg actacttgaa cgaatggggc aatcgcttca agaagctggc tgacatgtac
     5461 ggaggcggcg aggacgaccc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     5521 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5581 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5641 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5701 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5761 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5821 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5881 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5941 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     6001 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     6061 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     6121 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     6181 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaact cgagcctcta
     6241 gacagcgctc accggtcgtt aactaactta agctagcaac ggtttccctc tagcgggatc
     6301 aattccgccc ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     6361 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     6421 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     6481 gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     6541 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     6601 cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt
     6661 tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg
     6721 gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac
     6781 atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga
     6841 cgtggttttc ctttgaaaaa cacgataata ccatgattga acaagatgga ttgcacgcag
     6901 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     6961 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     7021 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     7081 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     7141 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     7201 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     7261 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     7321 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     7381 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     7441 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     7501 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     7561 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     7621 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccc gggcggggcg
     7681 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7741 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7801 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7861 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7921 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7981 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8041 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     8101 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8161 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8221 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     8281 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     8341 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     8401 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     8461 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     8521 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     8581 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     8641 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     8701 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     8761 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     8821 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     8881 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     8941 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9001 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9061 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9121 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9181 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     9241 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9301 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9361 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9421 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10081 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    11401 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
    11461 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11521 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11581 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11641 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11701 tatgtactac aaacttagta gt
//
