LOCUS       sequence-458300-       11976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Noordstra I, Hermoso MD, Schimmel L, Bonfim-Melo A, Currin-Ross D, 
            Duong CN, Kalappurakkal JM, Morris RG, Vestweber D, Mayor S, Gordon 
            E, Roca-Cusachs P, Yap AS
  TITLE     An E-cadherin-actin clutch translates the mechanical force of 
            cortical flow for cell-cell contact to inhibit epithelial cell 
            locomotion.
  JOURNAL   Dev Cell. 2023 Sep 25;58(18):1748-1763.e6. doi: 
            10.1016/j.devcel.2023.06.011. Epub 2023 Jul 21.
  PUBMED    37480844
REFERENCE   2  (bases 1 to 11976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.06.011"; journalName: "Dev Cell"; date: 
            "2023-09-25- 25"; volume: "58"; issue: "18"; pages: "1748-1763.e6"
COMMENT     Sequence Label: pLViN-iRFP670-alpha-cateninA+Delta-beta-H
FEATURES             Location/Qualifiers
     source          1..11976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2843..2852
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2849..3781
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    6575..7124
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6719..6736)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6946..6965
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7127..7921
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7181..7200)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7791..7810
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7945..8533
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7998..8018)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8416..8427)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8740..9373
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9501..9517)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9501..9517)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9514..9536)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9525..9541
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9549..9555,9556..9573,9574..9579))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9594..9615
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9732..9749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9903..10491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9983..10002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10662..11453,11454..11522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11285..11304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11523..11627)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11675..11809
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11712..11731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11766..11785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tagctagagg atcccccggg ctgcaggaat tcggcgcgcc
     2821 agtgaaccgt cagatccgct aggccaccat ggcgcgtaag gtcgatctca cctcctgcga
     2881 tcgcgagccg atccacatcc ccggcagcat tcagccgtgc ggctgcctgc tagcctgcga
     2941 cgcgcaggcg gtgcggatca cgcgcattac ggaaaatgcc ggcgcgttct ttggacgcga
     3001 aactccgcgg gtcggtgagc tactcgccga ttacttcggc gagaccgaag cccatgcgct
     3061 gcgcaacgca ctggcgcagt cctccgatcc aaagcgaccg gcgctgatct tcggttggcg
     3121 cgacggcctg accggccgca ccttcgacat ctcactgcat cgccatgacg gtacatcgat
     3181 catcgagttc gagcctgcgg cggccgaaca ggccgacaat ccgctgcggc tgacgcggca
     3241 gatcatcgcg cgcaccaaag aactgaagtc gctcgaagag atggccgcac gggtgccgcg
     3301 ctatctgcag gcgatgctcg gctatcaccg cgtgatgttg taccgcttcg cggacgacgg
     3361 ctccgggatg gtgatcggcg aggcgaagcg cagcgacctc gagagctttc tcggtcagca
     3421 ctttccggcg tcgctggtcc cgcagcaggc gcggctactg tacttgaaga acgcgatccg
     3481 cgtggtctcg gattcgcgcg gcatcagcag ccggatcgtg cccgagcacg acgcctccgg
     3541 cgccgcgctc gatctgtcgt tcgcgcacct gcgcagcatc tcgccctgcc atctcgaatt
     3601 tctgcggaac atgggcgtca gcgcctcgat gtcgctgtcg atcatcattg acggcacgct
     3661 atggggattg atcatctgtc atcattacga gccgcgtgcc gtgccgatgg cgcagcgcgt
     3721 cgcggccgaa atgttcgccg acttcttatc gctgcacttc accgccgccc accaccaacg
     3781 cggactcaga tctcgagctc atatgactgc tgtccatgca ggcaacataa acttcaagtg
     3841 ggatcctaaa agtctagaga tcaggactct ggcagttgag agactgttgg agcctcttgt
     3901 tacacaggtt acaacccttg taaacaccaa tagtaaaggg ccctctaata agaagagagg
     3961 tcgttctaag aaggcccatg ttttggctgc atctgttgaa caagcaactg agaatttctt
     4021 ggagaagggg gataaaattg cgaaggagag ccagtttctc aaggaggagc ttgtggctgc
     4081 tgtagaagat gttcgaaaac aaggtgattt gatgaaggct gctgcaggag agttcgcaga
     4141 tgatccctgc tcttctgtga agcgaggcaa catggttcgg gcagctcgag ctttgctctc
     4201 tgctgttacc cggttgctga ttttggctga catggcagat gtctacaaat tacttgttca
     4261 gctgaaagtt gtggaagatg gtatcttgaa gttgaggaat gctggcaatg aacaagactt
     4321 aggaatccag tataaagccc taaaacctga agtggataag ctgaacatta tggcagccaa
     4381 aagacaacag gaattgaaag atgttggcca tcgtgatcag atggctgcag ctagaggaat
     4441 cctgcagaag aacgttccga tcctctatac tgcatcccag gcatgcctac agcaccctga
     4501 tgtcgcagcc tataaggcca acagggacct gatatacaag cagctgcagc aggcggtcac
     4561 aggcatttcc aatgcagccc aggccactgc ctcagacgat gcctcacagc accagggtgg
     4621 aggaggagga gaactggcat atgcactcaa taactttgac aaacaaatca ttgtggaccc
     4681 cttgagcttc agcgaggagc gctttaggcc ttccctggag gagcgtctgg aaagcatcat
     4741 tagtggggct gccttgatgg ccgactcgtc ctgcacgcgt gatgaccgtc gtgagcgaat
     4801 tgtggcagag tgtaatgctg tccgccaggc cctgcaggac ctgctttcgg agtacatggg
     4861 caatgctgga cgtaaagaaa gaagtgatgc actcaattct gcaatagata aaatgaccaa
     4921 gaagaccagg gacttgcgta gacagctccg caaagctgtc atggaccacg tttcagattc
     4981 tttcctggaa accaatgttc cacttttggt attgattgaa gctgcaaaga atggaaatga
     5041 gaaagaagtt aaggagtatg cccaagtttt ccgtgaacat gccaacaaat tgattgaggt
     5101 tgccaacttg gcctgttcca tctcaaataa tgaagaaggt gtaaagcttg ttcgaatgtc
     5161 tgcaagccag ttagaagccc tctgtcctca ggttattaat gctgcactgg ctttagcagc
     5221 aaaaccacag agtaaactgg cccaagagaa catggatctt tttaaagaac aatgggaaaa
     5281 acaagtccgt gttctcacag atgctgtcga tgacattact tccattgatg acttcttggc
     5341 tgtctcagag aatcacattt tggaagatgt gaacaaatgt gtcattgctc tccaagagaa
     5401 ggatgtggat ggcctggacc gcacagctgg tgcaattcga ggccgggcag cccgggtcat
     5461 tcacgtagtc acctcagaga tggacaacta tgagccagga gtctacacag agaaggttct
     5521 ggaagccact aagctgctct ccaacacagt catgccacgt tttactgagc aagtagaagc
     5581 agccgtggaa gccctcagct cggaccctgc ccagcccatg gatgagaatg agtttatcga
     5641 tgcttcccgc ctggtatatg atggcatccg ggacatcagg aaagcagtgc tgatgataag
     5701 gacccctgag gagttggatg actctgactt tgagacagaa gattttgatg tcagaagcag
     5761 gacgagcgtc cagacagaag acgatcagct gatagctggc cagagtgccg ggagtggcag
     5821 tgctcagctt ccccaggagc aaaaagcgaa gattgcggaa caggtggcca gcttccagga
     5881 agaaaagagc aagctggatg ctgaagtgtc caaatgggac gacagtggca atgacatcat
     5941 tgtgctggcc aagcagatgt gcatgattat gatggagatg acagacttta cccgaggtaa
     6001 aggaccactc aaaaatacat cggatgtcat cagtgctgcc aagaaaattg ctgaggcagg
     6061 atccaggatg gacaagcttg gccgcaccat tgcagaccat tgccccgact cggcttgcaa
     6121 gcaggacctg ctggcctacc tgcaacgcat cgccctctac tgccaccagc tgaacatctg
     6181 cagcaaggtc aaggccgaat tcggggtgga cagcgccatg tccctgatcc aggcagccaa
     6241 gaacttgatg aatgctgtgg tgcagacagt gaaggcatcc tacgtcgcct ctaccaaata
     6301 ccaaaagtca cagggtatgg cttccctcaa ccttcctgct gtgtcatgga agatgaaggc
     6361 accagagaaa aagccattgg tgaagagaga gaaacaggat gagacacaga ccaagattaa
     6421 acgggcatct cagaagaagc acgtgaaccc ggtgcaggcc ctcagcgagt tcaaagctat
     6481 ggacagcatc taatgcagtc gacggtaccg cgttaactaa cttaagctag caacggtttc
     6541 cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa
     6601 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     6661 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     6721 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     6781 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     6841 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     6901 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     6961 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     7021 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
     7081 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatga ttgaacaaga
     7141 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     7201 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     7261 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     7321 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     7381 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     7441 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     7501 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     7561 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     7621 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     7681 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     7741 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     7801 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     7861 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     7921 acccgggcgg ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     7981 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     8041 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     8101 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     8161 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     8221 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     8281 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     8341 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     8401 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     8461 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     8521 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     8581 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     8641 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     8701 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     8761 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     8821 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     8881 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     8941 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     9001 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     9061 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     9121 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     9181 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     9241 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     9301 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     9361 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     9421 aaatgaatat cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc
     9481 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9541 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9601 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9661 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10081 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10321 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    11041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    11101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    11161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11641 aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg
    11701 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
    11761 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
    11821 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
    11881 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
    11941 ttgtatttgt taaatatgta ctacaaactt agtagt
//
