LOCUS       sequence-458301-        9158 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of LifeAct-iRFP670 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Noordstra I, Hermoso MD, Schimmel L, Bonfim-Melo A, Currin-Ross D, 
            Duong CN, Kalappurakkal JM, Morris RG, Vestweber D, Mayor S, Gordon 
            E, Roca-Cusachs P, Yap AS
  TITLE     An E-cadherin-actin clutch translates the mechanical force of 
            cortical flow for cell-cell contact to inhibit epithelial cell 
            locomotion.
  JOURNAL   Dev Cell. 2023 Sep 25;58(18):1748-1763.e6. doi: 
            10.1016/j.devcel.2023.06.011. Epub 2023 Jul 21.
  PUBMED    37480844
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2023.06.011"; journalName: "Dev Cell"; date: 
            "2023-09-25- 25"; volume: "58"; issue: "18"; pages: "1748-1763.e6"
COMMENT     Sequence Label: pLViP-LifeAct-iRFP670
FEATURES             Location/Qualifiers
     source          1..9158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        6..309
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        310..513
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     464..484
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             584..634
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
     CDS             650..1585
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     misc_feature    1676..2228
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1820..1837)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2047..2066
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2255..2854
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2255..2274)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2751..2771
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3746..3979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4051..4172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4088..4107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4142..4161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4212..4347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4367..4386)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4368..4386)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4396..4413)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4396..4412)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4405..4427)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4554..5009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4641..4660)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4851..4872
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5035..5139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5140..5208,5209..6000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5358..5377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6171..6759
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6660..6679
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6913..6930
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7047..7068
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7083..7088,7089..7106,7107..7113)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7121..7137
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7126..7148
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7145..7161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7145..7161
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7180..7200
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7182..7200
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7228..7454
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7455..7635
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7682..7807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8300..8533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8718..8762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9024..9141
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
       61 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      121 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      181 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      241 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      301 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      361 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      421 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      481 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
      541 cgctaccgga ctcagatctc gagctcaagc ttcgaattcc accatgggcg tggccgacct
      601 gatcaagaag ttcgagagca tcagcaagga ggagggggat ccaccggtta tggcgcgtaa
      661 ggtcgatctc acctcctgcg atcgcgagcc gatccacatc cccggcagca ttcagccgtg
      721 cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc acgcgcatta cggaaaatgc
      781 cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag ctactcgccg attacttcgg
      841 cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag tcctccgatc caaagcgacc
      901 ggcgctgatc ttcggttggc gcgacggcct gaccggccgc accttcgaca tctcactgca
      961 tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac aggccgacaa
     1021 tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt cgctcgaaga
     1081 gatggccgca cgggtgccgc gctatctgca ggcgatgctc ggctatcacc gcgtgatgtt
     1141 gtaccgcttc gcggacgacg gctccgggat ggtgatcggc gaggcgaagc gcagcgacct
     1201 cgagagcttt ctcggtcagc actttccggc gtcgctggtc ccgcagcagg cgcggctact
     1261 gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca gccggatcgt
     1321 gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc tgcgcagcat
     1381 ctcgccctgc catctcgaat ttctgcggaa catgggcgtc agcgcctcga tgtcgctgtc
     1441 gatcatcatt gacggcacgc tatggggatt gatcatctgt catcattacg agccgcgtgc
     1501 cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat cgctgcactt
     1561 caccgccgcc caccaccaac gctaatgtac agcatcttga gtcccttttt accgctgtta
     1621 ccaattttct tttgtctttg ggtaaacccg ggaattccgc ccctctcccc ccctaacgtt
     1681 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1741 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1801 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1861 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1921 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1981 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     2041 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     2101 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     2161 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     2221 tgataatatg gatatggcca caacggtcgc caccatgacc gagtacaagc ccacggtgcg
     2281 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     2341 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     2401 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     2461 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     2521 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     2581 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     2641 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     2701 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     2761 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     2821 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     2881 gaccgaaagg agcgcacgac cccatgcatg tattcaatct aagcaggctt tcactttctc
     2941 gccaacttac aaggcctttc tgtgtaaaca atacctgaac ctttaccccg ttgcccggca
     3001 acggccaggt ctgtgccaag tgtttgctga cgcaaccccc actggctggg gcttggtcat
     3061 gggccatcag cgcatgcgtg gaaccttttc ggctcctctg ccgatccata ctgcggaact
     3121 cctagccgct tgttttgctc gcagcaggtc tggagcaaac attatcggga ctgataactc
     3181 tgttgtccta tcccgcaaat atacatcgtt tccatggctg ctaggctgtg ctgccaactg
     3241 gatcctgcgc gggacgtcct ttgtttacgt cccgtcggcg ctgaatcctg cggacgaccc
     3301 ttctcggggt cgcttgggac tctctcgtcc ccttctccgt ctgccgttcc gaccgaccac
     3361 ggggcgcacc tctctttacg cggactcccc gtctgtgcct tctcatctgc cggaccgtgt
     3421 gcacttcgct tcacctctgc acgtcgcatg gagaccaccg tgaacgccca ccaaatattg
     3481 cccaaggtct tacataagag gactcttgga ctctcagcaa tgtcaacgac cgaccttgag
     3541 gcatacttca aagactgttt gtttaaagac tgggaggagt tgggggagga gattaggtta
     3601 aaggtctttg tactaggagg ctgtaggcat aaattggtct gcgcaccagc accatggcgc
     3661 aatcactaga gcggggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3721 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3781 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3961 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     4021 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     4081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     4141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     4201 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4261 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4321 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4381 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4441 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4501 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4561 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4621 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4681 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4741 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4801 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4861 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4921 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4981 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     5041 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5101 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5161 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5221 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5281 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5341 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5401 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5461 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5521 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5581 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5641 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5701 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5761 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5821 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5881 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5941 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6001 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6061 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     7021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     7081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     7141 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     7201 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     7261 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     7321 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     7381 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     7441 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     7501 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     7561 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     7621 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     7681 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     7741 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     7801 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     7861 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     7921 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     7981 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     8041 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     8101 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     8161 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     8221 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     8281 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     8341 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     8401 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     8461 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     8521 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     8581 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     8641 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     8701 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     8761 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     8821 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     8881 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca
     8941 ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag
     9001 tgaacggatc tcgacggtat cggttttaaa agaaaagggg ggattggggg gtacagtgca
     9061 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     9121 attacaaaaa ttcaaaattt tatcgataag cttgggag
//
