LOCUS       sequence-458307-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 
            transition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Qian Y, Celiker OT, Wang Z, Guner-Ataman B, Boyden ES
  TITLE     Temporally multiplexed imaging of dynamic signaling networks in 
            living cells.
  JOURNAL   Cell. 2023 Dec 7;186(25):5656-5672.e21. doi: 
            10.1016/j.cell.2023.11.010. Epub 2023 Nov 28.
  PUBMED    38029746
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11.010"; journalName: "Cell"; date: "2023-12-7-
            7"; volume: "186"; issue: "25"; pages: "5656-5672.e21"
COMMENT     Sequence Label: 
            pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             584..764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1433..1666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1851..1895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2193..2310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2369..2681
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2600..2620
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2698..2714
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2905..3208
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3209..3412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3444..4160
                     /codon_start=1
                     /product="reversibly switchable enhanced green fluorescent 
                     protein (Grotjohann et al., 2011)"
                     /label=rsEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTM
                     KFICTTGKLPVPWPTLVTTLTYGVLCFARYPDHMKQHDFFKSAMPEGYVQERTISFEDD
                     GYYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNLNSHNAYITADKQKNGIR
                     SNFKIRLNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQNKLSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     primer_bind     complement(3489..3510)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     4097..4118
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4167..4505
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVS"
     CDS             4536..4865
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     misc_feature    4870..5442
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5037..5054)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5264..5283
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5457..6131
                     /codon_start=1
                     /product="monomeric photoswitchable Dronpa-Green1 
                     fluorescent protein"
                     /label=Dronpa-Green1
                     /translation="MVSVIKPDMKIKLRMEGAVNGHPFAIEGVGLGKPFEGKQSMDLKV
                     KEGGPLPFAYDILTTVFCYGNRVFAKYPENIVDYFKQSFPEGYSWERSMNYEDGGICNA
                     TNDITLDGDCYIYEIRFDGVNFPANGPVMQKRTVKWEPSTEKLYVRDGVLKGDVNMALS
                     LEGGGHYRCDFKTTYKAKKVVQLPDYHFVDHHIEIKSHDKDYSNVNLHEHAEAHSELPR
                     QAK"
     CDS             6186..6458
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     protein_bind    complement(6489..6522)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6578..7166
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6631..6651)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7049..7060)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7169..7185)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             7408..7588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7650..8238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7730..7749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8409..9200,9201..9269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9032..9051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9270..9374)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1321 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1381 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1441 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1501 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1561 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1621 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1681 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1741 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1801 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1861 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1921 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1981 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2041 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2101 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2161 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2221 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2281 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2341 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     2401 ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2461 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2521 ttttaatact agctacattt tacatgatag gcttggattt ctataagaga tacaaatact
     2581 aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2641 tgttttgaga ctataaatat cccttggaga aaagccttgt taacgcgcgg tgaccctcga
     2701 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
     2761 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccgcgtg
     2821 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     2881 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2941 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3001 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3061 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3121 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3181 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3241 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3301 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3361 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3421 gaaccgtcag atccgctagc accatggtga gcaagggcga ggagctgttc accggggtgg
     3481 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg
     3541 agggcgaggg cgatgccacc aacggcaagc tgaccatgaa gttcatctgc accaccggca
     3601 agctgccagt gccctggccc accctcgtga ccaccctgac ctacggcgtg ctgtgcttcg
     3661 cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     3721 acgtccagga gcgcaccatc agcttcgagg acgacggcta ttacaagacc cgcgccgagg
     3781 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     3841 aggacggcaa catcctgggg cacaagctgg agtacaacct caacagccac aacgcctata
     3901 tcacggccga caagcagaag aacggcatca ggtctaactt caagatccgc ctcaacgtcg
     3961 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     4021 ccgtgctgct gcccgacaac cactacctga gcacccagaa taagctgagc aaagacccca
     4081 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgcctccggg atcactctcg
     4141 gcatggacga gctgtacaag ctgcaagttc aactggtgga gtcaggtggt gcacttgttc
     4201 agccgggcgg tagtttgcga ctttcatgcg cggcgtcagg tttccctgta aaccgataca
     4261 gtatgaggtg gtatagacaa gcgcccggca aagaacgaga atgggttgca ggaatgagta
     4321 gtgcaggaga tcgaagttcc tacgaagaca gcgttaaagg gcgcttcact atcagtcgag
     4381 atgatgcacg gaacaccgtg tatctgcaaa tgaacagcct taaacctgaa gatacggccg
     4441 tatattattg taacgtcaac gtcggatttg aatactgggg acagggtacg caagtcacgg
     4501 tatctgatat ccatgcacac tggcggccgc tcgagatgaa tcccagtatg aagcagaaac
     4561 aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact ctgaagatga
     4621 ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc gcaggcttgt
     4681 ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct ggggttattg
     4741 tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca tttgatctta
     4801 tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa cggagaaagg
     4861 cgctgtaagc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     4921 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     4981 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     5041 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     5101 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     5161 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     5221 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     5281 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     5341 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaacgtct aggccccccg
     5401 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatggccaca gaattcatgg
     5461 tgagtgtgat taaaccagac atgaagatca agctgcgtat ggaaggcgct gtaaatggac
     5521 acccgttcgc gattgaagga gttggccttg ggaagccttt cgagggaaaa cagagtatgg
     5581 accttaaagt caaagaaggc ggacctctgc ctttcgccta tgacatcttg acaactgtgt
     5641 tctgttacgg caacagggta ttcgccaaat acccagaaaa tatagtagac tatttcaagc
     5701 agtcgtttcc tgagggctac tcttgggaac gaagcatgaa ttacgaagac gggggcattt
     5761 gtaacgcgac aaacgacata accctggatg gtgactgtta tatctatgaa attcgatttg
     5821 atggtgtgaa ctttcctgcc aatggtccag ttatgcagaa gaggactgtg aaatgggagc
     5881 catccactga gaaattgtat gtgcgtgatg gagtgctgaa gggtgatgtt aacatggctc
     5941 tgtcgcttga aggaggtggc cattaccgat gtgacttcaa aactacttat aaagctaaga
     6001 aggttgtcca gttgccagac tatcactttg tggaccacca cattgagatt aaaagccacg
     6061 acaaagatta cagtaatgtt aatctgcatg agcacgccga agcgcattct gagctgccga
     6121 ggcaggccaa gtactcaatg ttgccttccc tgggcgatat ccatgcacac tggcggccgc
     6181 tcgagcccag ccccgccagg cccgcactcc gcgccccggc ctccgctacc agtggcagcc
     6241 gcaagcgcgc ccgcccgccc gccgcccccg gacgcgacca ggccaggcca ccggcccgca
     6301 ggagactgcg gctgtcggtg gacgaggttt ccagccccag tacccccgag gccccagaca
     6361 tcccagcctg cccttctccg ggccagaaga taaagaaatc caccccggca gcaggtcagc
     6421 cgccccacct gacatccgcg caggaccagg acaccatctg agtttaaacg aattcgtcga
     6481 gggacctaat aacttcgtat agcatacatt atacgaagtt atacatgttt aagggttccg
     6541 gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     6601 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     6661 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     6721 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     6781 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     6841 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     6901 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     6961 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     7021 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7081 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7141 gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga gacctagaaa
     7201 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     7261 aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct ttaagaccaa
     7321 tgacttacaa ggcagctgac gcgtgatttt aaaagaaaag gggggactgg aagggctaat
     7381 tcactcccac tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7501 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7561 ttttagtcag tgtggaaaat ctctagcagc atgtgagcaa aaggccagca aaaggccagg
     7621 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     7681 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     7741 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7801 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7861 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7921 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     7981 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8041 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8101 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8161 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8221 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8281 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8341 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     8401 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     8461 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     8521 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     8581 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     8641 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     8701 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     8761 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8821 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8881 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8941 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9001 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9061 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9121 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9181 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9241 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9301 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9361 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ac
//
