LOCUS       sequence-458323-        4369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  L0 part - CDS (without STOP codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Golubova D, Salmon M, Su H, Tansley C, Kaithakottil GG, Linsmith G, 
            Schudoma C, Swarbreck D, O'Connell MA, Patron NJ
  TITLE     Biosynthesis and bioactivity of anti-inflammatory triterpenoids in 
            Calendula officinalis.
  JOURNAL   Nat Commun. 2025 Jul 28;16(1):6941. doi: 10.1038/s41467-025-62269-w.
  PUBMED    40721615
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-07-28"; volume: "16(1):6941. doi"; pages: " 
            10.1038/s41467-025-62269-w"
COMMENT     Sequence Label: pEPMS0CM0036
FEATURES             Location/Qualifiers
     source          1..4369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2413..2429)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2413..2429)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2426..2448)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2601..3189)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2681..2700)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3322..3416)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(3437..4096)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     4011..4030
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(4097..4199)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4286..4308
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4300..4317
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4301..4317
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taggtctcta atgtggaaac taaagattgg tgaaaaaaat ggaaagcttg aaattggtga
       61 tggaaatggt aatgaatatc tctatagcac caacaatttt gttggaaggc aaatatggga
      121 gttcgacccc gatgcaggca cgcaagaaga acatgaccat atcgaaaggc ttcgagagca
      181 gttcttggtt agtaagaaga agctcggtat ccattgttgc ggtgacttgc ttatgcgtaa
      241 ccagcttatc aaagaaagtg ggattgatct tctaagtgaa cttccgatga gactagaaga
      301 tgaagatgtg aactacgaag cagtgtccac agcggttaga aaagctgtcc gactgaaccg
      361 ggcaattcaa gcttgggacg gtcattggcc ggctgaaaat tcgggtcccc tctttttcac
      421 ccctcctctg attattgcct tgtacatcag tggtacatta gatacaattt taacaaaaga
      481 acacaagaag gagatgatta gatacatgta catccatcaa aatgaagatg ggggatgggg
      541 tttttatata tcaggaaaaa gcacaatgat agggagtgct ttgaactatg tggctctaag
      601 acttcttgga gaagcttcac ctgatcatgt taacatggat gaagatcaac ttgctcgagc
      661 gcgtaaatgg atacttgatc atggtggcgc tacatccatc ccttcttggg gcaaagttta
      721 tctagcggta cttggtgtat atgaatggga agggtgcaac cctcttcctc cagaattttg
      781 gctttttcct tcctttttgc cttaccatcc agcaaaaatg tggtgttatt gcagaaccac
      841 atacatgcca atgtcacact tgtatggtaa aagctaccat ggccctatta ctgatcttgt
      901 tttgtctttg agaaaagaga ttcataccat tccttaccac cagattgatt ggaacaaaca
      961 acgacataac tgttgcaagg tggatctata ctaccctcac tctttcttac aagatctctt
     1021 atgggacagc cttcattatt ttactgaacc acttatgaaa agatggcctt tcaatagact
     1081 cataagacaa aagggactca aacgagtcct tgacctaatg cgttatggag ccgaggaagg
     1141 gcgttacatg gcacagggtt gtgttgacaa ggctttgcaa atcatgagtt tttatgctga
     1201 ggatccaaat gggatagact ttaaaagaca ccttgctaga atccctgatt acttgtgggt
     1261 ggccgaggac ggtatgaaga tgcaaagttt tggtagtcag ttgtgggatt gcgctctcgt
     1321 gactcaagcg attttggcaa gtgatatggt tgaggagtat ggagattcgc tcaagaaagc
     1381 tcacttttac ttgaaagagt cacagatcaa agagaatccc aaaggggatt tcacaaaaat
     1441 gtgtcgtcag tttacaaaag gggcttggac tttctctgat caagatcatg gatgggtagt
     1501 atcggattgc actgcagaag ctttgaagtg tttattggct ttgtcgcaaa tgccgcaaga
     1561 aatcgtagga gttaaggctg aagttgagcg attatatgag gctgtgaatg tcctacttta
     1621 cttacaaagt cctgaaagtg gtggttttgc aatttgggag ccaccagttc caaaaccata
     1681 tttacagatg ttgaatcctt cagaactttt tgctgacatt gtggttgaga aagaacacgt
     1741 tgaatgtacg ggctccataa ttcaagcttt gcaagcgttt aaagatttgc atgctgggca
     1801 tcgtgagata gaaatagaag ttgctatcgc gaaaggcata cgtttcttgg aaaacaagca
     1861 acaagatgat ggctcgtggt atggctattg gggtgtttgt tttctctatg gcacattttt
     1921 tgtgctgcaa gggttggtat cttgtgggaa aacatatgaa aataatgaag cagttcgaaa
     1981 aggtgtcaaa tttttgctct caacacagaa tttagaaggc ggttggggag agagctttga
     2041 atcatgccca aaagagaagt tcataccttt ggaggggaac cgaacaaatt tggtgcaaac
     2101 ttcatgggcc atgctcggtc ttatgttggg tggacaggcc gaaagggatc caacaccact
     2161 acacaaggca gcaaaattgc tcatcaatag ccagatggag aatggtgatt ttcctcaaca
     2221 ggaaataatg ggagtgtaca tgaaaaattg catgctacat tatccagaat acaggaacac
     2281 ttttccgtta tgggcgttgg gagaataccg taagcgtgtt tggctgccaa aacataaacc
     2341 cggttcgaga gacctaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
     2401 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
     2461 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     2521 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2581 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2941 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     3001 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3061 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3121 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3181 ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc
     3241 gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcccc ccatacgata
     3301 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     3361 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     3421 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     3481 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     3541 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     3601 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     3661 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     3721 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     3781 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     3841 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     3901 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     3961 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     4021 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     4081 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     4141 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     4201 cgtctcattg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     4261 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     4321 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
