LOCUS       sequence-458330-        5038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of a de novo designed, FRET-based 
            neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Chen JY-H, Shi Q, Peng X, Habimana JdD, Wang J, Sobolewski W, Yeh 
            AH-W
  TITLE     De novo luciferases enable multiplexed bioluminescence imaging.
  JOURNAL   Chem. 2025 Mar 13;11(3):102346. doi: 10.1016/j.chempr.2024.10.013. 
            Epub 2024 Nov 12.
  PUBMED    40417169
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Chem."; 
            date: "2025-03-13"; volume: "11(3):102346. doi"; pages: " 
            10.1016/j.chempr.2024.10.013. Epub 2024 Nov 12"
COMMENT     Sequence Label: pBAD-luxNeon
FEATURES             Location/Qualifiers
     source          1..5038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             1381..1398
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1439..1456)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      1609..1695
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1787..1814
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        1833..1924
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1925..1993,1994..2785)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2143..2162)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2956..3544
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3445..3464
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3698..3715
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3730..3870
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3796..3815)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3956..3978
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4134..5012)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gggctcagga acagacgaag aaatagcaga attcgtaaaa
      361 gcattctacg aagcactcga cgcaggagac gcagaaacag cagcagacct actattcgga
      421 gcaggatgca aagaaataca cctatgggac ggaacagtat tcacaagcaa agaaggattc
      481 gaaacatggt tcaaaaaact acaaagcgca gtaacaggag caaaaagaaa aatagaaagc
      541 gtagaaataa aagacggaga agcagtagta aaactaacac taacagcaac atacaaagca
      601 acaggaaaaa aattcgtagt agaactagaa cacacattca cattcgacag agaaagaaac
      661 gaactagtag aagtgaaagt cacagcaaca ccactgaata gcctgccggc gactcatgaa
      721 ctgcatattt ttggtagtat taacggcgtg gattttgata tggtgggtca gggcacgggc
      781 aacccgaacg atggctacga agaactgaac ctgaaatcta ccaaaggcga tcttcagttt
      841 agcccgtgga tcttagtgcc gcatattggt tatggttttc atcagtacct gccgtatccg
      901 gatggtatga gcccgtttca agcggcgatg gtggatggga gcggctatca ggtgcatcgc
      961 accatgcagt ttgaagatgg tgcaagcctg accgtgaact atcgttatac gtatgaaggc
     1021 agccatatta aaggtgaagc ccaggttaaa ggcaccggtt ttccggcgga tggcccggtg
     1081 atgaccaaca gcttaaccgc ggcagattgg tgtcgttcga aaaagaccta tccaaatgat
     1141 aaaaccatta ttagcacctt taaatggagc tacaccaccg gtaacggtaa acgctatcgc
     1201 agcaccgcgc gcaccaccta taccttcgcc aaaccgatgg cggcaaacta tctgaaaaac
     1261 cagccgatgt atgtgtttcg taaaaccgaa ttaaaacata gcaaaacgga attgaacttt
     1321 aaagaatggc agaaagcatt taccgatgtg atgggcatgg atgaattata taaaggttct
     1381 catcatcatc atcatcatta aagcttggct gttttggcgg atgagagaag attttcagcc
     1441 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     1501 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     1561 atggtagtgt ggagtaagcc catgcgagag tagggaactg ccaggcatca aataaaacga
     1621 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1681 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
     1741 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
     1801 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
     1861 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1921 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1981 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2041 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2101 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2161 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2221 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2281 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     2341 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     2401 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     2461 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     2521 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     2581 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     2641 tagtcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     2701 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2761 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2821 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2881 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2941 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3001 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3061 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3121 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3181 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3241 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3301 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     3361 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     3421 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     3481 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     3541 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     3601 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     3661 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3721 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     3781 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     3841 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     3901 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     3961 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat
     4021 tcgcgcgcga aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga agcagggatt
     4081 ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac
     4141 aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg
     4201 gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg
     4261 cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc ctggctgata
     4321 cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac
     4381 agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct
     4441 gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat cggtggatgg
     4501 agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc
     4561 agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg
     4621 ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc aaatattgac
     4681 ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac
     4741 cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa
     4801 atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc ctgaccgcga
     4861 atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga
     4921 taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa cgagtatccc
     4981 ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc attcagag
//
