LOCUS       sequence-458334-       10453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes SARS-CoV-2 variant XBB.1 Spike for pseudovirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Roederer AL, Cao Y, Denis KS, Sheehan ML, Li CJ, Lam EC, Gregory DJ,
            Poznansky MC, Iafrate AJ, Canaday DH, Gravenstein S, Garcia-Beltran 
            WF, Balazs AB
  TITLE     Ongoing evolution of SARS-CoV-2 drives escape from mRNA 
            vaccine-induced humoral immunity.
  JOURNAL   medRxiv [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815.
  PUBMED    38496628
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "medRxiv 
            [Preprint]. 2024 Mar 7:2024.03.05.24303815. doi: 
            10.1101/2024.03.05.24303815."
COMMENT     Sequence Label: pTwist-SARS-CoV-2 Spike-deltaC18 XBB.1
FEATURES             Location/Qualifiers
     source          1..10453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    3831..4419
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3884..3904)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4302..4313)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4472..4593
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4483..4502)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4537..4556
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4708..4754
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4784..4920
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    4965..5036
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     promoter        5081..5277
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5128..5263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5291..6085
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5345..5364)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5955..5974
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6096..6320
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(6516..7104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6596..6615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(7850..7918,7919..8710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8068..8087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      8723..8859
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    9011..9082
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(9087..9133)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    9151..9199
                     /note="synthetic polyadenylation signal"
     enhancer        9280..9583
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9585..9787
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9738..9758
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          9921..10396
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
ORIGIN
        1 catgtttgtg ttcctcgttc tcctgcccct cgtttcttcc caatgcgtga acctcataac
       61 tagaacgcag tcttacacga actcttttac tcgtggagta tactatcccg ataaagtctt
      121 taggtccagt gtccttcatt ctactcaaga ccttttcctg ccattcttct caaatgtcac
      181 ctggttccat gctatccacg tttctggcac caacggaact aaacgatttg ataatcccgc
      241 cttgccattt aacgatggcg tatactttgc ctcaacggag aagtccaaca ttatccgggg
      301 atggatattt ggcaccaccc tggactccaa gacacagtct ctcctcattg tgaacaacgc
      361 caccaatgtc gtcatcaaag tctgtgaatt tcaattttgt aacgatccct ttctcgatgt
      421 ttattatcag aagaataaca agtcttggat ggagtcagag ttccgcgttt acagttccgc
      481 taacaactgt acttttgagt acgtttccca accatttctc atggatcttg aagggaagga
      541 aggcaacttc aagaatctcc gcgaatttgt gtttaagaat atagacggct acttcaaaat
      601 ttatagcaag cataccccga taaatctgga gcgggatctc cctcaaggct tctccgcgtt
      661 ggaacccctc gtggacttgc cgattggcat caacatcacc cggttccaaa cccttcttgc
      721 ccttcatcga agctacctca cgcctgtcga cagtagtagt ggttggacag cgggcgcagc
      781 agcctactat gtgggatatt tgcaaccacg cacctttctt ctcaaatata acgaaaacgg
      841 aacaattacc gatgccgtag actgtgccct cgatcccctg tccgaaacta aatgcacact
      901 caagtccttc acagttgaga aaggcatata ccaaaccagt aacttccgcg tccaacccac
      961 cgaatccatt gttcgattcc ctaacatcac caatttgtgt cctttccatg aagtatttaa
     1021 tgccactaca tttgcatcag tttatgcctg gaatcgcaag cgaatatcaa attgtgttgc
     1081 agattatagt gtcatctata atttcgcacc attcttcgct tttaagtgtt atggggtcag
     1141 ccctacgaaa ttgaatgact tgtgttttac caacgtttac gcagattctt tcgtcatacg
     1201 cggcaacgaa gtcagccaga ttgcaccggg gcaaaccgga aatatagcag actataatta
     1261 taagttgccg gatgacttca ccggctgcgt tatagcatgg aactccaata agctcgattc
     1321 aaaacccagc ggcaactata attatcttta ccgcctgttt cgcaagtcaa agctgaagcc
     1381 ctttgaacgt gacatctcca ccgaaattta tcaagctggc aacaaaccat gtaatggagt
     1441 ggcagggtct aactgttatt ctccccttca atcatacgga tttcgaccaa catatggggt
     1501 gggccatcaa ccatatcggg tggtagtctt gagctttgag ctgctccacg caccggctac
     1561 agtttgcgga ccgaagaaaa gcactaacct ggtgaagaat aaatgtgtga acttcaactt
     1621 taatgggctc accggaacag gggtgctgac agaatcaaac aagaaatttc tcccttttca
     1681 acaatttggt agagacattg ccgataccac agatgccgtc cgcgatccgc aaacactgga
     1741 aatcctcgat ataacaccct gtagcttcgg cggggtcagc gtgataactc ccggaacgaa
     1801 cacatccaac caagtggctg tcctgtatca aggtgtcaac tgtaccgaag tccctgtggc
     1861 gatacacgcc gaccaactga cacccacctg gagagtctac tcaacaggtt ccaatgtctt
     1921 tcaaactaga gcgggttgtc tcattggcgc tgagtatgtc aataattcat acgaatgtga
     1981 tattcctatt ggggcaggaa tctgtgcgtc ttatcaaact caaacgaaat cacaccggcg
     2041 cgctcgctca gtagccagcc agtccataat cgcctatacc atgagtctcg gggctgagaa
     2101 ctccgtggct tattcaaaca attccatcgc aatcccaact aactttacca ttagcgttac
     2161 cacggaaatc ttgcccgtca gcatgactaa gacgagcgta gactgtacga tgtatatctg
     2221 tggggattcc accgagtgta gcaacctctt gcttcaatac gggtctttct gtactcaact
     2281 caaacgagca ttgaccggaa ttgcggtcga acaagacaag aatacccaag aggtgttcgc
     2341 acaagtaaag caaatataca aaacgccgcc aatcaaatat tttggcgggt ttaattttag
     2401 ccaaatactg ccagacccat ctaagccttc aaaacgcagc tttatcgagg atctgctgtt
     2461 caacaaagtt acgttggctg acgcgggttt cattaagcag tatggggatt gtttgggaga
     2521 cattgcggcc cgcgacctta tctgtgctca aaaattcaat gggttgacag ttctgccccc
     2581 tctgctcact gatgagatga ttgcacagta tacatcagca ttgttggcag gaaccattac
     2641 atccggctgg acttttggcg cgggtgcggc ccttcaaata cctttcgcta tgcaaatggc
     2701 ttacagattc aatgggattg gtgtcacgca aaatgtattg tatgaaaatc agaagctgat
     2761 cgctaatcaa tttaacagcg cgattggcaa gatccaagat tcactgtcaa gcacagcgtc
     2821 cgccctcggc aaactccaag atgttgtgaa tcataacgcc caggccctga ataccctcgt
     2881 gaagcaactg agttctaaat tcggcgcaat ctccagcgtc ctcaacgaca tattgtccag
     2941 acttgataag gtggaagccg aggtgcaaat tgatcggctc atcacaggca ggctccagtc
     3001 actccaaact tatgtgacac aacagttgat ccgggctgct gagatacgag caagcgccaa
     3061 cctggccgcc actaaaatgt cagagtgcgt gctcgggcag tccaaacggg ttgacttttg
     3121 tggcaagggc tatcacttga tgtccttccc ccaatccgcc ccacatggag tggtctttct
     3181 ccacgtcact tatgtccctg ctcaagagaa gaactttacg acagctccag caatctgtca
     3241 cgatggcaaa gcgcactttc ccagggaagg cgttttcgtc agtaatggca cgcattggtt
     3301 tgtgacccaa aggaatttct atgaacccca aatcataacc actgacaata catttgtctc
     3361 aggcaactgt gatgtggtca ttgggatcgt gaacaacacg gtatatgatc ccctgcaacc
     3421 cgagttggat agcttcaaag aggaattgga taaatacttt aagaatcata cttcacccga
     3481 tgttgatctc ggggacatat caggaataaa tgcaagtgtc gtcaacattc agaaagagat
     3541 tgatcgcctt aatgaggtcg ccaagaactt gaatgaatct ttgatcgact tgcaagaact
     3601 gggcaaatac gaacaatata ttaaatggcc ctggtacatt tggttgggct ttatcgccgg
     3661 acttatagct atcgttatgg tcacaattat gttgtgctgt atgactagct gttgttcctg
     3721 cttgaaagga tgttgcagtt gcggatcttg ttgtaaataa aggatcctct agaagttgtc
     3781 tcctcctgca ctgactgact gatacaatcg atttctggat ccgcaggcct aatcaacctc
     3841 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     3901 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     3961 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4021 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4081 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4141 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4201 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     4261 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4321 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4381 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtctagctt gactgactga
     4441 gatacagcgt accttcagct cacagacatg ataagataca ttgatgagtt tggacaaacc
     4501 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     4561 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4621 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     4681 ggtattggcc catctctatc ggtatcgtag cataacccct tggggcctct aaacgggtct
     4741 tgaggggttt tttgtgcccc tcgggccgga ttgctatcta ccggcattgg cgcagaaaaa
     4801 aatgcctgat gcgacgctgc gcgtcttata ctcccacata tgccagattc agcaacggat
     4861 acggcttccc caacttgccc acttccatac gtgtcctcct taccagaaat ttatccttaa
     4921 ggtcgtcagc tatcctgcag gcgatctctc gatttcgatc aagacattcc tttaatggtc
     4981 ttttctggac accactaggg gtcagaagta gttcatcaaa ctttcttccc tccctaatct
     5041 cattggttac cttgggctat cgaaacttaa ttaagcgatc tgcatctcaa ttagtcagca
     5101 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5161 tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5221 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagga
     5281 ggtagccaac atgattgaac aagatggatt gcacgcaggt tctcccgccg cttgggtgga
     5341 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     5401 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     5461 gaatgaactc caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     5521 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     5581 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     5641 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     5701 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     5761 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     5821 gatgcccgac ggcgaggatc tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat
     5881 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     5941 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6001 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6061 tcgccttctt gacgagttct tctagtatgt aagccctgtg ccttctagtt gccagccatc
     6121 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6181 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6241 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6301 ggatgcggtg ggctctatgg ttaattaacc agtcaagtca gctacttggc gagatcgact
     6361 tgtctgggtt tcgactacgc tcagaattgc gtcagtcaag ttcgatctgg tccttgctat
     6421 tgcacccgtt ctccgattac gagtttcatt taaatcatgt gagcaaaagg ccagcaaaag
     6481 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7321 tttcgttcat ccatagttgc atttaaattt ccgaactctc caaggccctc gtcggaaaat
     7381 cttcaaacct ttcgtccgat ccatcttgca ggctacctct cgaacgaact atcgcaagtc
     7441 tcttggccgg ccttgcgcct tggctattgc ttggcagcgc ctatcgccag gtattactcc
     7501 aatcccgaat atccgagatc gggatcaccc gagagaagtt caacctacat cctcaatccc
     7561 gatctatccg agatccgagg aatatcgaaa tcggggcgcg cctggtgtac cgagaacgat
     7621 cctctcagtg cgagtctcga cgatccatat cgttgcttgg cagtcagcca gtcggaatcc
     7681 agcttgggac ccaggaagtc caatcgtcag atattgtact caagcctggt cacggcagcg
     7741 taccgatctg tttaaaccta gatattgata gtctgatcgg tcaacgtata atcgagtcct
     7801 agcttttgca aacatctatc aagagacagg atcagcagga ggctttcgca tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcgc gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgctt
     8041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtattc
     8161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg gaggaccgaa
     8281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8401 ggcaacaacc ttgcgtaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8461 gttgatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8701 gcattggtaa ccgattctag gtgcattggc gcagaaaaaa atgcctgatg cgacgctgcg
     8761 cgtcttatac tcccacatat gccagattca gcaacggata cggcttcccc aacttgccca
     8821 cttccatacg tgtcctcctt accagaaatt tatccttaag atcccgaatc gtttaaactc
     8881 gactctggct ctatcgaatc tccgtcgttt cgagcttacg cgaacagccg tggcgctcat
     8941 ttgctcgtcg ggcatcgaat ctcgtcagct atcgtcagct tacctttttg gcagcgatcg
     9001 cggctcccga catcttggac cattagctcc acaggtatct tcttccctct agtggtcata
     9061 acagcagctt cagctacctc tcaattcaaa aaacccctca agacccgttt agaggcccca
     9121 aggggttatg ctatcaatcg ttgcgttaca cacacaaaaa accaacacac atccatcttc
     9181 gatggatagc gattttatta tctaactgct gatcgagtgt agccagatct agtaatcaat
     9241 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     9301 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     9361 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     9421 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     9481 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     9541 tacttggcag tacatctacg tattagtcat cgctattacc atgctgatgc ggttttggca
     9601 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     9661 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     9721 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     9781 cagagctggt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     9841 ccatagaaga caccgggacc gatccagcct cccctcgaag cttacatgtg gtaccgagct
     9901 cggatcctga gaacttcagg gtgagtctat gggacccttg atgttttctt tccccttctt
     9961 ttctatggtt aagttcatgt cataggaagg ggagaagtaa cagggtacac atattgacca
    10021 aatcagggta attttgcatt tgtaatttta aaaaatgctt tcttctttta atatactttt
    10081 ttgtttatct tatttctaat actttcccta atctctttct ttcagggcaa taatgataca
    10141 atgtatcatg cctctttgca ccattctaaa gaataacagt gataatttct gggttaaggc
    10201 aatagcaata tttctgcata taaatatttc tgcatataaa ttgtaactga tgtaagaggt
    10261 ttcatattgc taatagcagc tacaatccag ctaccattct gcttttattt tatggttggg
    10321 ataaggctgg attattctga gtccaagcta ggcccttttg ctaatcatgt tcatacctct
    10381 tatcttcctc ccacagctcc tgggcaacgt gctggtctgt gtgctggccc atcactttgg
    10441 caaaggcggc cgc
//
